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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1208 [new locus tag: SACOL_RS06190 ]
  • pan locus tag?: SAUPAN003476000
  • symbol: lspA
  • pan gene symbol?: lspA
  • synonym:
  • product: lipoprotein signal peptidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1208 [new locus tag: SACOL_RS06190 ]
  • symbol: lspA
  • product: lipoprotein signal peptidase
  • replicon: chromosome
  • strand: +
  • coordinates: 1215560..1216051
  • length: 492
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGCACAAAAAATATTTTATTGGCACTTCCATTTTAATAGCAGTATTTGTCGTTATATTT
    GACCAAGTTACTAAATATATTATAGCTACTACAATGAAAATTGGAGATTCATTTGAAGTG
    ATACCGCACTTTTTAAACATAACATCACATCGAAATAATGGTGCTGCATGGGGAATATTG
    AGTGGAAAAATGACATTTTTCTTTATTATTACCATTATTATATTAATAGCCTTAGTATAT
    TTCTTTATTAAAGATGCTCAATATAATTTGTTTATGCAAGTTGCTATTAGTTTACTTTTT
    GCAGGTGCACTTGGAAACTTTATTGATAGAATTTTAACAGGAGAAGTTGTTGACTTTATT
    GATACAAATATTTTTGGTTATGATTTTCCAATATTTAATATCGCAGATTCAAGTTTAACA
    ATTGGTGTAATATTAATTATTATTGCCTTATTAAAGGATACTTCCAATAAAAAGGAGAAG
    GAGGTTAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    492

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1208 [new locus tag: SACOL_RS06190 ]
  • symbol: LspA
  • description: lipoprotein signal peptidase
  • length: 163
  • theoretical pI: 7.75901
  • theoretical MW: 18341.6
  • GRAVY: 0.80184

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.23.36?  ExPASy
    Signal peptidase II Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains
  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal peptidase II (TIGR00077; EC 3.4.23.36; HMM-score: 135.8)
  • TheSEED  :
    • Lipoprotein signal peptidase (EC 3.4.23.36)
    Protein Metabolism Protein processing and modification Lipoprotein Biosynthesis  Lipoprotein signal peptidase (EC 3.4.23.36)
    and 2 more
    Protein Metabolism Protein processing and modification Signal peptidase  Lipoprotein signal peptidase (EC 3.4.23.36)
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Sex pheromones in Enterococcus faecalis and other Firmicutes  Lipoprotein signal peptidase (EC 3.4.23.36)
  • PFAM:
    no clan defined Peptidase_A8; Signal peptidase (SPase) II (PF01252; HMM-score: 123.9)
    and 3 more
    DUF2530; Protein of unknown function (DUF2530) (PF10745; HMM-score: 10.1)
    DUF4418; Domain of unknown function (DUF4418) (PF14387; HMM-score: 9.6)
    DUF3671; Protein of unknown function (PF12420; HMM-score: 7.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.236086
    • TAT(Tat/SPI): 0.000408
    • LIPO(Sec/SPII): 0.007547
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHFLNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAGALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEKEVK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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