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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1004 [new locus tag: SACOL_RS05130 ]
  • pan locus tag?: SAUPAN003170000
  • symbol: SACOL1004
  • pan gene symbol?: trfB
  • synonym:
  • product: competence protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1004 [new locus tag: SACOL_RS05130 ]
  • symbol: SACOL1004
  • product: competence protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1012048..1013034
  • length: 987
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTTAGTAGCTTTAAATGAAGAAAAGGAACGCGTATTAGCAACTACTGCATTGAGAAAG
    ACACAATATTTTTGTCCGGTGTGTGGCAAGCAAGTTATTTTAAAGCGTGGGCTCAAAGTA
    ATTAGTCATTTTGCACATAAACATTTAGCGGAACAAAAATGTTTTAATAATGAAACGATT
    AAACATTATAAAAGTAAATTGATTTTAGCACAGATGATACAGCAACAAGGATGTAAAGTA
    GAGATAGAGCCATTTTTAAAAGAAATAAAACAAATTCCGGATATTTTGATTAATAATAAA
    TATGTTATTGAGCTACAGTATTCGCCAATTCCTTATAAACAGATTCTTCAACGAACGGAA
    GGTTTAAAGAAAATGGGATATAAAGTAAGTTGGTTATTAAATGATGTTGATTATTGTCAT
    AATAAAGTGAAGTTCAATCATTTTCAAAGTATGTTTATTAATCCATTCACTCGAAAACTT
    CATACGTTCAATTTAGAGAAAAAACAAATAATGATGTTTCAACAAATACAATATTTAGGC
    GGGCACAAATATGTCGCTGAAAAAAGAAATGCCAAAATTAGTGAGTTGTTTAATGAGGCG
    CCTTGTGATTATCATGCTGTTTATAAATTATCAAAGTTCGCAATTAATCAATATATCAAA
    TATTGTCGCTGGCAAAATTCTGTTTTAGAACCCACTTTAAGTGCAATGTATCAATTACAG
    TTAACTGATCAAGAAGTAGTGCACAATTATGGTTATATTTTTCCAGAGCAAATTTATATT
    AAAAATCATCCTATTGAGTGGCAATTACAAGTTGATTTATGGTTAAAGAATGGAAAAAGC
    AAATTAGTAAATGACAATCTTAATTATTTTAAACTGAAAAAATTTATTGTTGCTCTAGAA
    AGTAAAACAGCAATTATAGAAAAACTTATTAACAATTATTTAAATATTTGTTCAGATAAA
    GGTAATGACGTGCAAATTTTGTTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    987

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1004 [new locus tag: SACOL_RS05130 ]
  • symbol: SACOL1004
  • description: competence protein
  • length: 328
  • theoretical pI: 9.71608
  • theoretical MW: 38840.3
  • GRAVY: -0.335671

⊟Function[edit | edit source]

  • TIGRFAM:
    acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 14.3)
  • TheSEED  :
    • Competence protein CoiA
    DNA Metabolism DNA uptake, competence Gram Positive Competence  Competence protein CoiA
  • PFAM:
    PDDEXK (CL0236) CoiA; Competence protein CoiA-like family (PF06054; HMM-score: 104.4)
    and 1 more
    Zn_Beta_Ribbon (CL0167) NOB1_Zn_bind; Nin one binding (NOB1) Zn-ribbon like (PF08772; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006438
    • TAT(Tat/SPI): 0.001641
    • LIPO(Sec/SPII): 0.003812
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHFAHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGCKVEIEPFLKEIKQIPDILINNKYVIELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPFTRKLHTFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKISELFNEAPCDYHAVYKLSKFAINQYIKYCRWQNSVLEPTLSAMYQLQLTDQEVVHNYGYIFPEQIYIKNHPIEWQLQVDLWLKNGKSKLVNDNLNYFKLKKFIVALESKTAIIEKLINNYLNICSDKGNDVQILF

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Suzanne E Dale, Amanda Doherty-Kirby, Gilles Lajoie, David E Heinrichs
Role of siderophore biosynthesis in virulence of Staphylococcus aureus: identification and characterization of genes involved in production of a siderophore.
Infect Immun: 2004, 72(1);29-37
[PubMed:14688077] [WorldCat.org] [DOI] (P p)

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