From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0707 [new locus tag: SACOL_RS03635 ]
  • pan locus tag?: SAUPAN002528000
  • symbol: SACOL0707
  • pan gene symbol?: dhaK
  • synonym:
  • product: dihydroxyacetone kinase subunit DhaK

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0707 [new locus tag: SACOL_RS03635 ]
  • symbol: SACOL0707
  • product: dihydroxyacetone kinase subunit DhaK
  • replicon: chromosome
  • strand: +
  • coordinates: 732688..733653
  • length: 966
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAAGTTAATCAATAAAAAAGAAACATTTTTAACTGATATGCTTGAAGGATTGTTA
    ATTGCGCACCCAGAGTTAGATCTGATTGCTAATACAGTTATTGTAAAAAAAGCTAAGAAA
    GAACATGGTGTAGCAATAGTCTCTGGAGGTGGAAGCGGACATGAACCTGCGCATGCCGGT
    TTTGTTGCAGAAGGTATGCTAGATGCAGCGGTTTGTGGCGAAGTATTTACATCACCTACA
    CCTGATAAAATATTAGAAGCTATTAAAGCAGTAGATACTGGTGATGGTGTATTACTAGTT
    GTAAAAAACTATGCAGGTGACGTGATGAATTTCGAAATGGCACAAGAGCTTGCAGAAATG
    GAAGGTATAAATGTTCAAACTGTTATTGTTCGTGACGACATTGCTGTGACAAACGAAGTA
    CAACGTCGTGGTGTTGCAGGAACAGTGTTTGTTCATAAGCTTGCCGGTTATCTTGCTGAA
    AAAGGTTATTCATTAACAGAGATAAAATCGCGTGTAGAAGCGTTGTTACCTGAAATTAAA
    AGTATTGGTATGGCAATTGAGCCACCGCTTGTTCCAACTACTGGAAAATATGGCTTTGAT
    ATTGAAGACGACAAAATGGAAATCGGTATTGGTATACATGGTGAAAAAGGTATTCATAGG
    GAAGAAGTAAAGGATATTGATCATATTGTTGGAACATTGTTAGACGAATTGTATAAAGAA
    GTTACTGCCAATGATGTCATATTAATGGTAAATGGTATGGGTGGTACGCCGTTATCTGAA
    TTAAATATCGTAACTAAATATATTCAACAAAATTTAGCTGCAAGAACGGTTAATGTTGCT
    AAATGGTTTGTTGGTGATTATATGACATCTTTAGACATGCAAGGTTTTTCTATAACTATC
    GTGCCTAATAAACCAGAATATTTGGAAGCATTTTTAGCACCAACAACAAGTCAATACTTT
    AAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    966

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0707 [new locus tag: SACOL_RS03635 ]
  • symbol: SACOL0707
  • description: dihydroxyacetone kinase subunit DhaK
  • length: 321
  • theoretical pI: 4.70202
  • theoretical MW: 34887.1
  • GRAVY: 0.0551402

⊟Function[edit | edit source]

  • TIGRFAM:
    dihydroxyacetone kinase, DhaK subunit (TIGR02363; EC 2.7.1.-; HMM-score: 479.3)
    and 3 more
    dihydroxyacetone kinase (TIGR02361; EC 2.7.1.29; HMM-score: 311.7)
    probable dihydroxyacetone kinase DhaK1b subunit (TIGR02362; HMM-score: 263)
    dihydroxyacetone kinase, phosphotransfer subunit (TIGR02364; HMM-score: 14.2)
  • TheSEED  :
    • Phosphoenolpyruvate-dihydroxyacetone phosphotransferase (EC 2.7.1.121), dihydroxyacetone binding subunit DhaK
    Carbohydrates Central carbohydrate metabolism Dihydroxyacetone kinases  Phosphoenolpyruvate-dihydroxyacetone phosphotransferase (EC 2.7.1.121), dihydroxyacetone binding subunit DhaK
  • PFAM:
    EDD (CL0245) Dak1; Dak1 domain (PF02733; HMM-score: 384)
    and 2 more
    EIIA-man; PTS system fructose IIA component (PF03610; HMM-score: 14.6)
    no clan defined DUF3139; Protein of unknown function (DUF3139) (PF11337; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013832
    • TAT(Tat/SPI): 0.00115
    • LIPO(Sec/SPII): 0.000857
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHGVAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVVKNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLAEKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKGIHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAARTVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL0707&oldid=55411"