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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0526 [new locus tag: SACOL_RS02690 ]
  • pan locus tag?: SAUPAN002219000
  • symbol: SACOL0526
  • pan gene symbol?: holB
  • synonym:
  • product: DNA polymerase III subunit delta'

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0526 [new locus tag: SACOL_RS02690 ]
  • symbol: SACOL0526
  • product: DNA polymerase III subunit delta'
  • replicon: chromosome
  • strand: +
  • coordinates: 537584..538510
  • length: 927
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATGAACAGCAACAATTGACGAATGCATATCATTCAAATAAATTATCGCATGCCTAT
    TTATTTGAAGGTGATGATGCACAAACGATGAAACAAGTTGCGATTAATTTTGCAAAGCTT
    ATTTTATGTCAAACAGATAGTCAATGTGAAACAAAGGTTAGTACATATAATCATCCAGAC
    TTTATGTATATATCAACAACTGAGAATGCAATTAAGAAAGAACAAGTTGAACAACTTGTG
    CGTCATATGAATCAACTTCCTATAGAAAGCACAAATAAAGTGTACATCATTGAAGACTTT
    GAAAAGTTAACTGTTCAAGGGGAAAACAGTATCTTGAAATTTCTTGAAGAACCACCGGAC
    AATACGATTGCTATTTTATTGTCTACAAAACCTGAGCAAATTTTAGACACAATCCATTCA
    AGGTGTCAGCATGTATATTTCAAGCCTATTGATAAAGAAAAGTTTATAAATAGATTAGTT
    GAACAAAACATGTCTAAGCCAGTAGCTGAAATGATTAGTACTTATACTACGCAAATAGAT
    AATGCAATGGCTTTAAATGAAGAATTTGATTTATTAGCATTAAGGAAATCAGTTATACGT
    TGGTGTGAATTGTTGCTTACTAATAAGCCAATGGCACTTATAGGTATTATTGATTTATTG
    AAACAGGCTAAAAATAAAAAACTGCAATCTTTAACTATTGCAGCTGTGAATGGTTTCTTC
    GAAGATATCATACATACAAAGGTAAATGTAGAGGATAAACAAATATATAGTGATTTAAAA
    AATGATATTGATCAATATGCGCAAAAGTTGTCGTTTAATCAATTAATTTTGATGTTTGAT
    CAACTGACGGAAGCACATAAGAAATTGAATCAAAATGTAAATCCAACGCTTGTATTTGAA
    CAAATCGTAATTAAGGGTGTGAGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    927

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0526 [new locus tag: SACOL_RS02690 ]
  • symbol: SACOL0526
  • description: DNA polymerase III subunit delta'
  • length: 308
  • theoretical pI: 5.41475
  • theoretical MW: 35515.6
  • GRAVY: -0.314286

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta' subunit (TIGR00678; EC 2.7.7.7; HMM-score: 153.4)
    and 8 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, subunit gamma and tau (TIGR02397; EC 2.7.7.7; HMM-score: 102.8)
    Cellular processes Cellular processes Sporulation and germination ATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 18.7)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 18.7)
    radical SAM/SPASM domain protein, ACGX system (TIGR04340; HMM-score: 14.9)
    mutatrotase, YjhT family (TIGR03547; EC 5.1.-.-; HMM-score: 13.6)
    Cellular processes Cellular processes Sporulation and germination ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 13.1)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 13.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone naphthoate synthase (TIGR01929; EC 4.1.3.36; HMM-score: 11.7)
  • TheSEED  :
    • DNA polymerase III delta prime subunit (EC 2.7.7.7)
    DNA Metabolism DNA replication DNA-replication  DNA polymerase III delta prime subunit (EC 2.7.7.7)
    and 1 more
    Stress Response Heat shock Heat shock dnaK gene cluster extended  DNA polymerase III delta prime subunit (EC 2.7.7.7)
  • PFAM:
    P-loop_NTPase (CL0023) DNA_pol3_delta2; DNA polymerase III, delta subunit (PF13177; HMM-score: 155.9)
    and 2 more
    post-AAA (CL0604) DNApol3-delta_C; DNA polymerase III, delta subunit, C terminal (PF09115; HMM-score: 22.9)
    DNA_pol3_gamma3; DNA polymerase III subunits gamma and tau domain III (PF12169; HMM-score: 20.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.032526
    • TAT(Tat/SPI): 0.004331
    • LIPO(Sec/SPII): 0.003335
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQTDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEKLTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLVEQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIIDLLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLILMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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