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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2122 [new locus tag: SA_RS12195 ]
  • pan locus tag?: SAUPAN005818000
  • symbol: hutU
  • pan gene symbol?: hutU
  • synonym:
  • product: urocanate hydratase

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2122 [new locus tag: SA_RS12195 ]
  • symbol: hutU
  • product: urocanate hydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 2386381..2388042
  • length: 1662
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAGAAAAATTCAAGCAAAAAAAGGTTTGAGTATTGAGTGTAAAGGTTGGGAACAAGAA
    GCAGTACTTAGAATGTTATACAACAATTTAGACCCAGAAGTTGCTGAAAGACCAGAAGAT
    TTAGTTGTTTATGGTGGTATTGGAAAAGCAGCACGTAATTGGGAAGCTTTTGAAGCAATA
    GAGAAAACGTTACGAGAATTAGAATCAGATGAGACAATGTTAGTACAATCAGGAAAGCCA
    GTTGCTGTATTTAAAACACATGAAGAAGCGCCACGTGTATTAATTTCAAATTCAGTATTA
    GTGCCTGAATGGGCAAACTGGGATCATTTTAATGAATTAGATAAAAAAGGTTTAATCATG
    TATGGACAGATGACAGCCGGTAGTTGGATATATATTGGTTCTCAAGGTATTGTGCAAGGT
    ACTTATGAGACATTTGCCGAGCTAGGTAATCAACACTTTAATGGAGATTTAGCAGGCACT
    GTTACACTGACAGCAGGTTTAGGTGGTATGGGTGGTGCGCAACCGTTAGCAATTACTATG
    AATCATGGGGTAGCAATTTGCGTGGATGTTGATGAAACACGTGTTGATAAGCGAATTGAT
    ACGAAATACTGTGATGTTAAAACAGCTGATTTAGATGAAGCATTAAAATTAGCAGAAGAG
    GCGAAAGAACGTGGAGAAGGATTATCAATCGGGTTAGTTGGAAATGCTGTAGACATCCAT
    CAAGCGATTCTAGAAAAAGGATTTAAAATTGACATTATTACTGACCAAACAAGTGCACAT
    GATCCGCTAAATGGATATGTGCCACAAGGATACTCTGTAGAAGAAGCGAAAGTATTGCGT
    GAAAAAGATCCGAAAAAATATGTTGAACTGTCACAAGCTTCAATGGCAAAGCATGTTGAA
    TTAATGCTTGAATTCCAAAAACGTGGCGCTGTAGCATTTGATTATGGTAACAATATTCGT
    CAAGTAGCCTTCAATAACGGAGTGAAAAATGCTTTTGACTTCCCAGGCTTTGTACCAGCT
    TACATTAGACCATTATTCTGTGAAGGTAAAGGGCCATTCCGCTTTGCTGCGTTGAGTGGT
    GATCCAAAAGATATCGAGCGTGCGGATGAAGAAATGCGCAAACTTTTCCCAGAAAATGAA
    AAGTTATTAAGATGGCTTGATTTAGCTGAAGAAAAAATTTCATATCAAGGACTACCATCA
    CGTATTGCTTGGTTAGGCTATGGAGAAAGAGCGAAAATGGGCTTAGCTTTAAATCGTCTT
    GTACGTGACGGTGAAATTTCAGCACCAATTGTTATTGGACGAGACCATTTAGATGCTGGT
    TCAGTTGCTAGTCCTAACCGTGAAACAGAAAGTATGAAAGATGGCAGTGACGCGGTTGGT
    GATTGGGCTGTATTGAATGCACTCATTAACACAGCTGCAGGTGGTTCATGGATTTCATTC
    CATCACGGTGGCGGTGTTGGCATGGGATATTCACTTCATGCGGGTATGGTTGTTGTAGCA
    GATGGATCAGAGCGTGCTGAAAGAAGATTGGAACGTGTATTGACGACTGATCCAGGTATG
    GGTGTTGCCCGACATGTTGATGCTGGCTATGACATCGCTATTCAAACAGCTAAAGAAAAA
    GGTATTCATATTCCAATGATTGATAAAGCAGGTGATAAATAA
    60
    120
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    840
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    1020
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    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1662


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2122 [new locus tag: SA_RS12195 ]
  • symbol: HutU
  • description: urocanate hydratase
  • length: 553
  • theoretical pI: 5.03205
  • theoretical MW: 60632.4
  • GRAVY: -0.292586

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.49?  ExPASy
    Urocanate hydratase 3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines urocanate hydratase (TIGR01228; EC 4.2.1.49; HMM-score: 969.4)
  • TheSEED  :
    • Urocanate hydratase (EC 4.2.1.49)
    Amino Acids and Derivatives Histidine Metabolism Histidine Degradation  Urocanate hydratase (EC 4.2.1.49)
  • PFAM:
    NADP_Rossmann (CL0063) Urocanase; Urocanase Rossmann-like domain (PF01175; HMM-score: 314.5)
    no clan defined Urocanase_C; Urocanase C-terminal domain (PF17392; HMM-score: 298.2)
    and 3 more
    Urocanase_N; Urocanase N-terminal domain (PF17391; HMM-score: 202.8)
    DUF6429; Domain of unknown function (DUF6429) (PF20008; HMM-score: 13.6)
    Exonuc_VII_S; Exonuclease VII small subunit (PF02609; HMM-score: 8.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: NAD+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.946
    • Cytoplasmic Membrane Score: 0.0009
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.053
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00465
    • TAT(Tat/SPI): 0.000226
    • LIPO(Sec/SPII): 0.00071
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVYGGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVPEWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGTVTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLAEEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEAKVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDFPGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEKISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETESMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAERRLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HutR* (activation) regulon, CcpA regulon
    HutR*(TF)important in Histidine utilization; RegPrecise 
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]


⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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