PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1917 [new locus tag: SA_RS11025 ]
• pan locus tag^?: SAUPAN005406000
• symbol: SA1917
• pan gene symbol^?: ptpB
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1917 [new locus tag: SA_RS11025 ]
• symbol: SA1917
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2165946..2166365
• length: 420
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124818 NCBI
• RefSeq: NP_375222 NCBI
• BioCyc: see SA_RS11025
• MicrobesOnline: 104248 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  
  ATGAAGATTTTATTCGTTTGTACAGGTAACACATGTCGTAGCCCATTAGCGGAAAGTATT
  GCAAAAGAGGTTATGCCAAATCATCAATTTGAATCAAGAGGTATATTCGCTGTGAACAAT
  CAAGGTGTTTCGAATTATGTTGAAGACTTAGTTGAAGAACATCATTTAGCTGAAACGACC
  TTATCGCAACAATTTACTGAAGCAGATTTGAAAGCAGATATTATTTTGACGATGTCGTAT
  TCGCACAAAGAATTAATAGAGGCACACTTTGGTTTGCAAAATCATGTTTTCACATTGCAT
  GAATATGTAAAAGAAGCAGGAGAAGTTATAGATCCATACGGTGGAACAAAAGAAATGTAT
  GTACATACCTATGAAGAACTTGTAAGTTTAATTTTAAAATTAAAAGATATTATTTGCTAG
  60
  120
  180
  240
  300
  360
  420
  

⊟Protein

⊟General

• locus tag: SA1917 [new locus tag: SA_RS11025 ]
• symbol: SA1917
• description: hypothetical protein
• length: 139
• theoretical pI: 4.81841
• theoretical MW: 15787.9
• GRAVY: -0.109353

⊟Function

• reaction:
  EC 3.1.3.48?  ExPASy
  Protein-tyrosine-phosphatase Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate
• TIGRFAM:
  Cellular processes Cellular processes Detoxification arsenate reductase (thioredoxin) (TIGR02691; EC 1.20.4.-,3.1.3.48; HMM-score: 28.3)
  and 1 more
  Cellular processes Cellular processes Detoxification arsenate reductase, glutathione/glutaredoxin type (TIGR02689; EC 1.20.4.1; HMM-score: 14.6)
• TheSEED  :
  • Protein arginine phosphatase (EC 3.9.1.2) PtpB
  CBSS-176280.1.peg.1561  Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48)
  and 2 more
  LMPTP YfkJ cluster  Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48)
  LMPTP YwlE cluster  Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48)
• PFAM:
  Phosphatase (CL0031) LMWPc; Low molecular weight phosphotyrosine protein phosphatase (PF01451; HMM-score: 82.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.029213
  • TAT(Tat/SPI): 0.000338
  • LIPO(Sec/SPII): 0.006351
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927689 NCBI
• RefSeq: NP_375222 NCBI
• UniProt: Q7A4E1 UniProt

⊟Protein sequence

• MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVSNYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEYVKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1917 < SA1918 < SA1919 < prfA < tdk

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Somnath Mukherjee, Riddhiman Dhar, Amit Kumar Das
Analyzing the catalytic mechanism of protein tyrosine phosphatase PtpB from Staphylococcus aureus through site-directed mutagenesis.
Int J Biol Macromol: 2009, 45(5);463-9
[PubMed:19747503] [WorldCat.org] [DOI] (I p)