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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1721 [new locus tag: SA_RS09880 ]
  • pan locus tag?: SAUPAN004934000
  • symbol: pcrA
  • pan gene symbol?: pcrA
  • synonym:
  • product: ATP-dependent DNA helicase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1721 [new locus tag: SA_RS09880 ]
  • symbol: pcrA
  • product: ATP-dependent DNA helicase
  • replicon: chromosome
  • strand: -
  • coordinates: 1970502..1972694
  • length: 2193
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAATGCGTTATTAAATCATATGAATACAGAGCAAAGTGAAGCTGTAAAGACAACAGAA
    GGACCATTGTTAATTATGGCAGGTGCTGGTTCAGGGAAGACACGTGTTTTAACACATAGA
    ATTGCTTATTTATTAGACGAAAAAGATGTCTCACCATACAATGTTTTGGCTATTACTTTT
    ACAAATAAAGCTGCAAGAGAAATGAAAGAACGTGTTCAAAAATTAGTAGGTGATCAAGCA
    GAAGTTATTTGGATGTCAACATTCCACTCAATGTGTGTTCGTATTTTACGTCGTGATGCA
    GATCGAATAGGTATAGAACGCAATTTTACGATAATTGATCCTACAGACCAAAAATCTGTT
    ATTAAAGACGTCTTAAAAAATGAAAATATTGATAGTAAAAAGTTTGAACCTCGTATGTTT
    ATCGGTGCGATCAGTAATTTGAAAAATGAACTTAAAACACCTGCAGATGCTCAAAAAGAA
    GCCACAGATTATCACTCGCAAATGGTAGCAACGGTTTATAGTGGATATCAACGCCAATTG
    TCGCGTAATGAAGCGTTAGATTTTGATGACCTTATTATGACAACGATTAACTTATTTGAG
    CGTGTACCAGAAGTTCTAGAATATTATCAGAACAAATTCCAATATATTCATGTAGATGAG
    TATCAAGATACTAATAAAGCACAATATACATTAGTTAAATTATTAGCAAGTAAGTTTAAA
    AACTTATGTGTTGTAGGTGACTCAGATCAGTCAATTTATGGTTGGCGTGGTGCTGATATT
    CAAAATATCTTATCATTTGAAAAAGACTACCCAGAAGCGAATACAATCTTTTTAGAGCAA
    AATTATCGTTCGACGAAAACGATTTTAAATGCGGCTAACGAAGTGATTAAAAATAATTCT
    GAACGTAAGCCAAAAGGACTGTGGACTGCAAATACGAATGGTGAGAAAATTCATTACTAT
    GAAGCAATGACAGAACGTGATGAAGCGGAATTTGTAATACGAGAAATTATGAAGCATCAA
    CGTAATGGTAAGAAATATCAAGATATGGCAATTTTATATAGAACAAATGCACAATCACGT
    GTACTTGAGGAAACATTCATGAAATCTAATATGCCATACACAATGGTTGGTGGCCAAAAG
    TTCTATGACCGTAAAGAAATCAAAGATTTATTAAGTTATTTGCGTATAATTGCCAATAGT
    AATGATGACATTAGTTTGCAACGTATTATTAATGTTCCAAAAAGAGGTGTAGGTCCTTCA
    TCTGTTGAAAAAGTTCAAAACTATGCACTTCAAAACAATATCAGTATGTTTGATGCACTT
    GGAGAAGCTGATTTTATCGGATTGTCAAAAAAGGTGACACAAGAGTGTCTTAATTTTTAC
    GAGTTAATCCAAAGCTTGATAAAAGAACAAGAATTTTTAGAAATTCATGAAATAGTTGAT
    GAAGTATTACAAAAGTCTGGCTACCGAGAAATGCTTGAACGTGAAAATACACTAGAATCT
    CGAAGTAGATTAGAAAACATCGATGAATTTATGTCAGTACCAAAAGACTATGAGGAAAAT
    ACCCCATTAGAAGAACAGTCATTAATTAACTTTTTAACGGATTTATCGTTAGTAGCTGAT
    ATTGATGAGGCAGATACTGAAAATGGCGTAACACTAATGACGATGCACTCGGCTAAGGGC
    CTTGAATTTCCAATCGTCTTTATAATGGGGATGGAAGAATCTTTATTCCCACATATTAGA
    GCGATTAAGAGTGAAGATGATCACGAAATGCAAGAAGAACGTCGTATTTGTTATGTAGCA
    ATTACAAGGGCTGAAGAGGTGTTATATATCACTCATGCGACATCAAGAATGTTATTTGGT
    CGCCCTCAGTCGAATATGCCATCTAGATTTTTAAAGGAAATTCCAGAATCACTATTAGAA
    AATCATTCAAGTGGCAAACGACAAACGATACAACCTAAGGCAAAACCTTTTGCTAAACGC
    GGATTTAGTCAACGAACAACGTCAACGAAAAAACAAGTATCGTCATCTGATTGGAATGTA
    GGTGACAAAGTGATGCATAAAGCCTGGGGAGAAGGCATGGTGAGTAATGTAAACGAGAAA
    AATGGCTCAATCGAACTAGATATTATCTTTAAATCACAAGGGCCAAAACGTTTGTTAGCG
    CAATTTGCACCAATTGAAAAAAAGGAGGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2193

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1721 [new locus tag: SA_RS09880 ]
  • symbol: PcrA
  • description: ATP-dependent DNA helicase
  • length: 730
  • theoretical pI: 5.63681
  • theoretical MW: 84046.9
  • GRAVY: -0.55274

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicase ATP + H2O = ADP + phosphate
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase PcrA (TIGR01073; EC 3.6.4.12; HMM-score: 1190.6)
    and 5 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA helicase II (TIGR01075; EC 3.6.4.12; HMM-score: 738.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase Rep (TIGR01074; EC 3.6.4.12; HMM-score: 660.8)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair helicase-exonuclease AddAB, AddA subunit (TIGR02785; EC 3.1.-.-,3.6.4.12; HMM-score: 206.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair double-strand break repair helicase AddA (TIGR02784; HMM-score: 159.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair exodeoxyribonuclease V, beta subunit (TIGR00609; EC 3.1.11.5; HMM-score: 157.2)
  • TheSEED  :
    • ATP-dependent DNA helicase UvrD/PcrA (EC 3.6.4.12)
    DNA Metabolism DNA repair DNA repair, bacterial UvrD and related helicases  ATP-dependent DNA helicase UvrD/PcrA
  • PFAM:
    P-loop_NTPase (CL0023) UvrD-helicase; UvrD/REP helicase N-terminal domain (PF00580; HMM-score: 292.8)
    UvrD_C; UvrD-like helicase C-terminal domain (PF13361; HMM-score: 267.3)
    and 9 more
    AAA_19; AAA domain (PF13245; HMM-score: 122.7)
    Viral_helicase1; Viral (Superfamily 1) RNA helicase (PF01443; HMM-score: 38)
    UvrD_C_2; UvrD-like helicase C-terminal domain (PF13538; HMM-score: 37.5)
    AAA_30; AAA domain (PF13604; HMM-score: 33.2)
    Exonuc_V_gamma; Exodeoxyribonuclease V, gamma subunit (PF04257; HMM-score: 20.4)
    AAA_11; AAA domain (PF13086; HMM-score: 18.1)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 16.3)
    Vps51 (CL0295) Vps51; Vps51/Vps67 (PF08700; HMM-score: 14.4)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00559
    • TAT(Tat/SPI): 0.001019
    • LIPO(Sec/SPII): 0.001743
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYLLDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADRIGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKEATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHVDEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTIFLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIREIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLSYLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSKKVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENIDEFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIVFIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQSNMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVSSSDWNVGDKVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA2023(rpoA)DNA-directed RNA polymerase subunit alpha  [2] (data from MRSA252)
    SA0501(rpoC)DNA-directed RNA polymerase subunit beta'  [2] (data from MRSA252)
    SA0993(uvrC)excinuclease ABC subunit C  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.0 2.1 2.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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