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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1525 [new locus tag: SA_RS08585 ]
  • pan locus tag?: SAUPAN004351000
  • symbol: dnaE
  • pan gene symbol?: dnaE
  • synonym:
  • product: DNA polymerase III subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1525 [new locus tag: SA_RS08585 ]
  • symbol: dnaE
  • product: DNA polymerase III subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 1740948..1744145
  • length: 3198
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGTGGCATATTTAAATATTCATACGGCTTATGATTTGTTAAATTCAAGCTTAAAAATA
    GAAGATGCCGTAAGACTTGCTGTGTCTGAAAATGTTGATGCACTTGCCATAACTGACACC
    AATGTATTGTATGGTTTTCCTAAATTTTATGATGCATGTATAGCAAATAACATTAAACCG
    ATTTTTGGTATGACAATATATGTGACAAATGGATTAAATACAGTCGAAACAGTTGTTCTA
    GCTAAAAATAATGATGGATTAAAAGATTTGTATCAACTATCATCGGAAATAAAAATGAAT
    TCAATGGAAAATGTTTCATTTGAACTATTGCAACAATTTTCATCGAATTTGATTATCATT
    TTTAAAAATGTTGCAGACGAACATCGTGACATTGTTCAAGTTTTTGATTCGCATGAAGAT
    ACGTATTTAGATCATCAAAGTGTTTTAGTTCAGGGTATAAAGCACGTATGGATTCAAAAT
    GTTTGTTACCAAACACGTCAAGATGCCGATACGATTTCTGCATTAGCAGCTATTAGAGAC
    AATGCAAAATTAGACTTAATTCATGATCAAGAAGATTTTGGTGCACATTTTTTAACTGAA
    AAGGAAATTAAACAATTAGATATTAACCAAGAATATTTAACGCAGGTTGATGTTATAGCT
    CAAAAGTGTAATGCAGAATTAAAATATCATCAATCTCTACTTCCTCAATATCAGACACCT
    AACGATGAATCAGCTAAAAAATATTTGTGGCGTGTCTTAGTTACACAATTGAAAAAATTA
    GAACTTAATTATGACGTCTATTTAGAGCGATTGAAATATGAGTATAAAGTTATTACTAAT
    ATGGGTTTTGAAGATTATTTCTTAATAGTAAGTGATTTAATCCATTATGCGAAAACGAAT
    GATGTGATGGTAGGTCCAGGTCGTGGTTCTTCAGCTGGCTCACTGGTCAGTTATTTACTA
    GGTATTACAACGATTGATCCTATTAAATTCAATCTATTATTTGAACGTTTTTTAAACCCA
    GAACGTGTAACAATGCCTGATATAGATATTGACTTTGAAGATACACGCCGAGAAAGGGTT
    ATTCAGTACGTCCAAGAAAAATATGGCGAGCTACATGTATCTGGAATTGTGACTTTCGGT
    CATCTGCTTGCAAGAGCGGTTGCTAGGGATGTTGGACGAATTATGGGGTTTGATGAAGTT
    ACATTAAATGAAATTTCAAGTTTAATCCCACATAAATTAGGAATTACACTTGATGAAGCA
    TATCAAATTGACGATTTTAAAAAATTTGTACATCGAAACCATCGACATGAACGCTGGTTC
    AGTATTTGTAAAAAGTTAGAAGGTTTACCAAGACATACATCTACACATGCGGCAGGAATT
    ATTATTAATGACCATCCATTATATGAATATGCCCCTTTAACGAAAGGGGATACAGGATTA
    TTAACGCAATGGACAATGACTGAAGCCGAACGAATTGGGTTATTAAAAATAGATTTTCTA
    GGGTTGAGAAACTTATCGATTATTCATCAAATCTTAATACAAGTCAAAAAAGATTTAGGT
    ATTAATATTGATATCGAAAAGATTCCGTTCGATGATCAAAAAGTGTTTGAATTGTTGTCG
    CAAGGAGATACGACTGGCATATTCCAATTAGAGTCAGACGGTGTAAGAAGTGTATTAAAA
    AAATTAAAGCCGGAACACTTTGAAGATATTGTTGCTGTAACTTCTTTGTATAGACCAGGT
    CCAATGGAAGAAATTCCAACTTACATTACAAGAAGACATGATCCAAGCAAAGTTCAATAT
    TTACATCCACATTTAGAACCTATATTAAAAAATACTTACGGTGTTATTATTTATCAAGAG
    CAAATTATGCAAATAGCGAGCACATTTGCAAACTTCAGTTATGGTGAAGCGGATATTTTA
    AGAAGAGCAATGAGTAAAAAAAATAGAGCTGTTCTTGAAAGTGAGCGTCAACATTTTATA
    GAAGGTGCAAAGCAAAATGGTTATCACGAAGACATTAGTAAGCAAATATTTGATTTGATT
    CTGAAATTTGCTGATTATGGTTTTCCTAGAGCACATGCTGTCAGCTATTCTAAAATTGCA
    TACATTATGAGCTTTTTAAAAGTCCATTATCCAAATTATTTTTACGCAAATATTTTAAGT
    AATGTTATTGGAAGTGAGAAGAAAACTGCTCAAATGATAGAAGAAGCAAAAAAACAAGGT
    ATCACTATATTGCCACCGAACATTAACGAAAGTCATTGGTTTTATAAACCTTCCCAAGAA
    GGCATTTATTTATCAATTGGTACAATTAAAGGTGTTGGTTATCAAAGTGTGAAAGTGATT
    GTTGATGAACGTTATCAGAACGGCAAATTTAAAGATTTCTTTGATTTTGCTAGACGTATA
    CCGAAGAGAGTCAAAACGAGAAAGTTACTTGAAGCACTGATTTTAGTGGGAGCGTTTGAT
    GCTTTTGGTAAAACACGTTCAACGTTGTTGCAAGCTATTGATCAAGTGTTGGATGGCGAT
    TTAAACATTGAACAAGATGGTTTTTTATTTGATATTTTAACGCCAAAACAGATGTATGAA
    GATAAAGAAGAATTGCCTGATGCACTTATTAGTCAGTACGAAAAAGAATATTTAGGATTT
    TATGTTTCGCAACACCCAGTAGATAAAAAGTTTGTTGCCAAACAATATTTAACGATATTT
    AAATTGAGTAACGCGCAGAATTATAAACCTATATTAGTACAGTTTGATAAAGTTAAACAA
    ATTCGAACTAAAAATGGTCAAAATATGGCATTCGTCACATTAAATGATGGCATTGAAACT
    TTAGATGGTGTGATTTTCCCTAATCAGTTTAAAAAGTACGAAGAGTTGTTATCACATAAT
    GACTTGTTTATAGTTAGCGGGAAATTTGACCATAGAAAGCAACAACGTCAACTAATTATA
    AATGAGATTCAGACATTAGCCACTTTTGAAGAACAAAAATTAGCATTTGCCAAACAAATT
    ATAATTAGAAATAAATCACAAATAGATATGTTTGAAGAGATGATTAAAGCTACGAAAGAG
    AATGCTAATGATGTTGTGTTATCCTTTTATGATGAAACGATTAAACAAATGACTACTTTA
    GGCTATATTAATCAAAAAGATAGTATGTTTAATAATTTTATACAATCCTTTAACCCTAGT
    GATATTAGGCTTATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3198

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1525 [new locus tag: SA_RS08585 ]
  • symbol: DnaE
  • description: DNA polymerase III subunit alpha
  • length: 1065
  • theoretical pI: 6.28318
  • theoretical MW: 122835
  • GRAVY: -0.265822

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit (TIGR00594; EC 2.7.7.7; HMM-score: 932.9)
    and 1 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 79.9)
  • TheSEED  :
    • DNA polymerase III alpha subunit, class I (EC 2.7.7.7)
    DNA Metabolism DNA replication DNA-replication  DNA polymerase III alpha subunit (EC 2.7.7.7)
  • PFAM:
    NTP_transf (CL0260) DNA_pol3_alpha; Bacterial DNA polymerase III alpha subunit (PF07733; HMM-score: 552.8)
    and 6 more
    HHH (CL0198) HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 81.1)
    Amidohydrolase (CL0034) PHP; PHP domain (PF02811; HMM-score: 80.9)
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 33.2)
    RRM (CL0221) Transposase_22; L1 transposable element RBD-like domain (PF02994; HMM-score: 17.9)
    OB (CL0021) DUF4539; Domain of unknown function (DUF4539) (PF15072; HMM-score: 13.3)
    HTH (CL0123) KORA; TrfB plasmid transcriptional repressor (PF16509; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009238
    • TAT(Tat/SPI): 0.000331
    • LIPO(Sec/SPII): 0.001788
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLYGFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNSMENVSFELLQQFSSNLIIIFKNVADEHRDIVQVFDSHEDTYLDHQSVLVQGIKHVWIQNVCYQTRQDADTISALAAIRDNAKLDLIHDQEDFGAHFLTEKEIKQLDINQEYLTQVDVIAQKCNAELKYHQSLLPQYQTPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYKVITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLFERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVGRIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLPRHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSIIHQILIQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEHFEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQIASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILKFADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQGITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFARRIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPKQMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQFDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHRKQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFYDETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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