PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1487 [new locus tag: SA_RS08370 ]
• pan locus tag^?: SAUPAN004274000
• symbol: folC
• pan gene symbol^?: folC
• synonym:
• product: folylpolyglutamate synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1487 [new locus tag: SA_RS08370 ]
• symbol: folC
• product: folylpolyglutamate synthase
• replicon: chromosome
• strand: -
• coordinates: 1692659..1693930
• length: 1272
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124331 NCBI
• RefSeq: NP_374774 NCBI
• BioCyc: see SA_RS08370
• MicrobesOnline: 103800 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGAATTACCTAGAGAGCTTGTATTGGATACACGAAAGAACTAAATTTGGCATCAAACCA
  GGTGTTAAACGTATGGAATGGATGCTAGCACAATTTAATAATCCTCAAAATAACATTAAG
  GGTATTCATGTAGGTGGCACAAATGGTAAAGGCTCTACAGTTGCTTACCTTAGAACAGCT
  TTAGTTGAAAATGGTTATGAAGTAGGTACATTTACGTCGCCGTTTATTGAAACATTTAAT
  GAACGAATTAGTCTAAATGGTGTGCCAATATCAAATGACGCTATTGTAGAATTAGTATCA
  CGTATTAAACCAGTAAGTGAAATGATGGAACGTGAAACAGATTTAGGTGTTGCAACTGAA
  TTCGAAATAATCACAGCGATGATGTTTTTATATTTTGGTGAAATACATCCTGTTGATTTT
  GTCATTGTTGAGGCTGGATTGGGTATAAAGAACGATTCGACAAATGTCTTTACACCGGTT
  TTATCAATCTTAACTAGTATCGGTCTAGACCATACAGATATTTTAGGTGGTACTTATCTA
  GATATTGCTAGGGATAAAGGCGCGATTATAAAGCCTAACGTTCCAGTGATATATGCTGTT
  AAAAATGAAGATGCATTAAAATATGTTCGTGAACGCGCAATTGAACAACATGCAAAGCCA
  ATTGAATTAGATAGAGAAATTGTTGTTGTATCGCAAAATGATGAATTTACTTACCGTTAT
  AAAGATTATGAATTAGAAACAATCATTTTAAGCATGTTAGGTGAACATCAGAAACAAAAT
  GCTGCATTAGCCATAACAGCTCTTATTGAATTAAATGAACAAGGATTAATTGAATTAGAT
  TTCAATAAGATGATAGACGGTATTGAATCAGTTCGTTGGACTGGACGTATTGAGCAGGTG
  CATGACAAACCTTTAATCATTTTGGATGGCGCACATAATTCAGAGAGTATAGATGCTCTA
  ATTGATACAATTAAACAGTACCATGATAAAGAAAAAGTAGATATTTTGTTCTCAGCAATA
  AACGGAAAACCGATTAACGAGATGGTCAAACATTTAAGTTTAATTGCGCATACGTTTTAT
  GCAACTGAATTTGATTTTCCGAAAGCGTTACGCAAAGAAGAAATTGTAGGTAGTATTGAA
  AATGATGAAATACAATTAGTAGATGACTACGTTGAATTTATAAAAAATTATCAAGGTGAT
  ACATTAGTAATTACCGGTAGTCTGTATTTCATAAGTGAAGTTAAATCAAAATTAGACTTT
  GAAGCATTATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1272
  

⊟Protein

⊟General

• locus tag: SA1487 [new locus tag: SA_RS08370 ]
• symbol: FolC
• description: folylpolyglutamate synthase
• length: 423
• theoretical pI: 4.55771
• theoretical MW: 47856.3
• GRAVY: -0.0959811

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 376.6)
  and 5 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 55)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 41.2)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 35.7)
  Cell structure Cell envelope Other poly-gamma-glutamate synthase PgsB (TIGR04012; EC 6.3.2.-; HMM-score: 19.7)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 15.1)
• TheSEED  :
  • Dihydrofolate synthase (EC 6.3.2.12)
  • Folylpolyglutamate synthase (EC 6.3.2.17)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Dihydrofolate synthase (EC 6.3.2.12)
  and 3 more
  Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Folylpolyglutamate synthase (EC 6.3.2.17)
  Virulence, Disease and Defense Bacteriocins, ribosomally synthesized antibacterial peptides Colicin V and Bacteriocin Production Cluster  Dihydrofolate synthase (EC 6.3.2.12)
  Virulence, Disease and Defense Bacteriocins, ribosomally synthesized antibacterial peptides Colicin V and Bacteriocin Production Cluster  Folylpolyglutamate synthase (EC 6.3.2.17)
• PFAM:
  no clan defined Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 42.8)
  and 3 more
  Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 27.5)
  ACT (CL0070) ALS_ss_C; Small subunit of acetolactate synthase (PF10369; HMM-score: 13.8)
  P-loop_NTPase (CL0023) AAA_14; AAA domain (PF13173; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.022144
  • TAT(Tat/SPI): 0.000469
  • LIPO(Sec/SPII): 0.001971
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927241 NCBI
• RefSeq: NP_374774 NCBI
• UniProt: A0A0H3JUH2 UniProt

⊟Protein sequence

• MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHVGGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRIKPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPVLSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHAKPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGLIELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVDILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVEFIKNYQGDTLVITGSLYFISEVKSKLDFEAL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: folC < valS

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications