PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0314 [new locus tag: SA_RS01810 ]
• pan locus tag^?: SAUPAN001857000
• symbol: SA0314
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0314 [new locus tag: SA_RS01810 ]
• symbol: SA0314
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 371544..372344
• length: 801
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123093 NCBI
• RefSeq: NP_373560 NCBI
• BioCyc: see SA_RS01810
• MicrobesOnline: 102586 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGAAACGTTAAAATCAAATAAAGCGAGACTTGAATATTTAATCAATGATATGCGTCGA
  GAGAGAAATGACAATGACGTATTGGTAATGCCATCTTCATTTGAAGATTTGTGGGAATTA
  TATCGAGGCTTGGCAAATGTCAGACCGGCATTACCTGTAAGTGATGAATATTTAGCTGTA
  CAAGATGCTATGTTAAGTGATTTGAATCATCAACATGTTACGGATTTGAAGGATTTGAAG
  CCGATAAAAGGTGACAATATCTTTGTTTGGCAAGGTGATATCACGACGTTAAAAATCGAT
  GCTATTGTTAATGCTGCAAATAGTCGTTTTCTAGGATGTATGCAAGCTAATCATGACTGC
  ATTGATAATATTATTCATACAAAAGCGGGTGTTCAAGTTCGACTTGATTGTGCAGAGATC
  ATTCGACAACAAGGGCGCAATGAAGGTGTAGGTAAAGCCAAAAAAACACGTGGATATAAT
  TTGCCAGCAAAGTATATAATTCATACGGTTGGTCCGCAAATACGTCGATTGCCTGTTTCA
  AAGATGAATCAGGACTTGTTAGCTAAATGTTATCTTAGCTGTCTTAAATTGGCTGATCAA
  CATAGTTTAAATCATGTCGCTTTTTGCTGTATATCTACAGGTGTATTTGCTTTTCCTCAA
  GATGAAGCAGCAGAAATTGCTGTTCGAACAGTAGAAAGCTATCTCAAAGAAACAAATTCA
  ACATTGAAAGTCGTGTTCAATGTATTTACAGATAAGGATTTACAACTGTATAAGGAGGCA
  TTGAACCGTGATGCAGAGTAG
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⊟Protein

• 5KIV
  PDB

Protein Data Bank: 5KIV

⊟General

• locus tag: SA0314 [new locus tag: SA_RS01810 ]
• symbol: SA0314
• description: hypothetical protein
• length: 266
• theoretical pI: 6.85834
• theoretical MW: 30109.3
• GRAVY: -0.292857

⊟Function

• reaction:
  EC 3.2.1.-?  ExPASy
  4-(ADP-D-ribosyl)-protein-L-aspartate + H₂O = ADP-D-ribose + protein-L-aspartate?
• TIGRFAM:
• TheSEED  :
  • Protein-ADP-ribose hydrolase
• PFAM:
  MACRO (CL0223) Macro; Macro domain (PF01661; HMM-score: 122.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8774
  • Cytoplasmic Membrane Score: 0.0152
  • Cell wall & surface Score: 0.001
  • Extracellular Score: 0.1064
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001623
  • TAT(Tat/SPI): 0.000221
  • LIPO(Sec/SPII): 0.000216
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926027 NCBI
• RefSeq: NP_373560 NCBI
• UniProt: P67344 UniProt

⊟Protein sequence

• METLKSNKARLEYLINDMRRERNDNDVLVMPSSFEDLWELYRGLANVRPALPVSDEYLAVQDAMLSDLNHQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAIVNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKKTRGYNLPAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAFPQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEALNRDAE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0312 > SA0313 > SA0314 > SA0315 > SA0316

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Johannes Gregor Matthias Rack, Rosa Morra, Eva Barkauskaite, Rolf Kraehenbuehl, Antonio Ariza, Yue Qu, Mary Ortmayer, Orsolya Leidecker, David R Cameron, Ivan Matic, Anton Y Peleg, David Leys, Ana Traven, Ivan Ahel
Identification of a Class of Protein ADP-Ribosylating Sirtuins in Microbial Pathogens.
Mol Cell: 2015, 59(2);309-20
[PubMed:26166706] [WorldCat.org] [DOI] (I p)