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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS14600 [old locus tag: NWMN_2541 ]
- pan locus tag?: SAUPAN006373000
- symbol: NWMN_RS14600
- pan gene symbol?: pmi
- synonym:
- product: mannose-6-phosphate isomerase, class I
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS14600 [old locus tag: NWMN_2541 ]
- symbol: NWMN_RS14600
- product: mannose-6-phosphate isomerase, class I
- replicon: chromosome
- strand: +
- coordinates: 2797620..2798558
- length: 939
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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901ATGGCATTATTTTTACAACCCGTTTTTAAAGACAGAATTTGGGGCGGTCATGCATTAAAA
GCATTTAACTATGATATCCCTAATGAAACAACTGGTGAATGCTGGGCAATTTCTGCACAT
CCAAATGGTCCTAATACAATTATCAATGGTCCATACAAGGATATGACCCTAGATCAACTT
TGGTCACAACATCGTGAATTATTCGATAATGATTCACGAGATAGTTTCCCTCTGCTAACT
AAAGTATTAGATGCCAATGATAAATTATCTGTTCAAGTTCACCCAGATGATGACTACGCT
TTAAAACACGAAGGCGAACTAGGTAAAACAGAATGTTGGTATATTTTAGATGCAGCCCCA
GGTGCTGAAATTATATATGGCGTACATGCACATAATAAACAGACGTTAATAGACATGATT
GATAATCATGAATTTGACAGTCTTTTCAAACGTATACCTGTTAAGCCCGGTGACTTTTAT
TATGTTCCTGCTGGCACTGTCCATGCCATTGGTTCGGGTATTTTAATTTTAGAGACACAA
CAATCCTCCGATACGACATACCGTATTTATGACTATGATAGACGAGATCAAAATGGTCAA
TTGCGTGATTTGCACTTAGAGCAAAGTAAAGATGTGATTGAATTAGGCAATCATGAACCA
AATACTACTCCTATAACAACTCACATCGACACACACACTGTAACTCAATTTGTAGATAAT
CAATATTTTGCAGTATATAAATGGGATATTCATGGAGATTTAAAATTCAATAAACCACAT
GCTTATTGCTTAGTTACAATTATCGATGGTACTGGTACTTTAACAGTAAACGATAAGCAC
TATGACATCCAAAAAGGTTCTAGCTTTATTTTAACTACGGAAGACGAGGATATTCACTTT
GTCGGTAATATTAGTGCTATAGTAAGTCATCCTGGATAA60
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939
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS14600 [old locus tag: NWMN_2541 ]
- symbol: NWMN_RS14600
- description: mannose-6-phosphate isomerase, class I
- length: 312
- theoretical pI: 4.94021
- theoretical MW: 35395.2
- GRAVY: -0.461538
⊟Function[edit | edit source]
- TIGRFAM: Energy metabolism Sugars mannose-6-phosphate isomerase, class I (TIGR00218; EC 5.3.1.8; HMM-score: 226.2)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: Cupin (CL0029) PMI_typeI; Phosphomannose isomerase type I (PF01238; HMM-score: 158.9)and 2 moreCupin_2; Cupin domain (PF07883; HMM-score: 37.7)AraC_binding; AraC-like ligand binding domain (PF02311; HMM-score: 25)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Zn2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.050223
- TAT(Tat/SPI): 0.002634
- LIPO(Sec/SPII): 0.008036
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGPNTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALKHEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFYYVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNHEPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTVNDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CcpA see NWMN_2541
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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