PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS13880 [old locus tag: NWMN_2414 ]
• pan locus tag^?: SAUPAN006159000
• symbol: NWMN_RS13880
• pan gene symbol^?: fbp
• synonym:
• product: fructose 1,6-bisphosphatase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS13880 [old locus tag: NWMN_2414 ]
• symbol: NWMN_RS13880
• product: fructose 1,6-bisphosphatase
• replicon: chromosome
• strand: +
• coordinates: 2657325..2659289
• length: 1965
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2657325..2659289) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2414

⊟Phenotype

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⊟DNA sequence

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  ATGACTCAAATTACTGAAAAAGAATTAAAAAAGAAGTATTTAGATTTACTATCCCAAAAT
  TTTGATACTCCAGAAAAACTTGCAACTGAAATTATCAATTTAGAATCAATTTTAGAATTA
  CCTAAAGGTACGGAACATTTCGTCAGCGATTTACATGGTGAATATGAAGCTTTCCAACAC
  GTATTACGCAACGGTTCTGGGAACGTGCGAGCGAAAATCAATGATATTTTCAAAGAGAGA
  CTTTCAACTAAGGAGCTTAATGACTTAACTGCTCTTGTCTACTATCCAGAAGACAAATTA
  AAATTGATTAAAAGTGATTTCCAAAATTGCGGTCAACTTAATGTCTGGTATATCACAACA
  ATCGAACATTTAATTGAGTTAATTAAATATTGTTCTTCAAAATATACGCGTTCAAAACTG
  CGTAAAGCATTGCCAAAACAATACGTTTATATTATTGAGGAACTACTGTATAAAAGTAAT
  GAATATCAAAATAAAAAATCATATTACGAAACACTTGTTAACCAAGTAATTGAACTTAAA
  CAGGCAGATGATTTAATTATTGGACTCGCTTATTCCGTACAACGCTTAGTCGTCGATCAT
  TTACACGTTGTCGGTGATATTTATGATCGTGGACCACAACCAGATAAAATTATGGATACA
  CTGATTAATTATCATTCCCTAGATATTCAATGGGGTAATCATGATGTGCTTTGGGTTGGA
  GCCTATGCTGGGTCAAAAGTATGCTTAGCAAACTTACTTCGAATTTGTGCACGTTATGAT
  AATTTAGATATTATCGAAGACGCTTATGGCATTAATTTAAGACCACTGCTTACTTTAGCT
  GAAAAATACTATGACGCAGATAATCCTGCTTTTAAGCCTAAAAAAAGACCTGACAAACAC
  GAACGTTTAACTCAACGTGAAGAAAGTCAAATTACTAAAATTCATCAAGCTATTGCGATG
  ATTCAATTCAAGTTAGAAATACCAATTATTAAACGTCGTCCAAATTTCGAAATGGAAGAA
  CGTCTTGTGCTTGAAAAGGTTAATTATGATACAAATGAAATTACAGTTTATGGTAATACA
  TACCCATTGAAAGACACATGTTTCCAAACTGTCAATCGTGATAATCCAGCAGAATTACTA
  CCTGAAGAAGAAGAAGTCATGAATAAACTATTATTGTCATTCCAACAATCTGAAAAATTA
  CGTCGTCATATGTCTTTCTTGATGCGTAAAGGCTCTCTTTACTTACCATATAATGGCAAT
  TTACTCATTCATGGTTGTATTCCAGTTGATGAAAATGGTGAGATGGAATCATTTGAAATT
  GATGGTCATACTTACAGCGGCCAAGAATTATTAGATGTGTTTGAGTATCATGTCCGTAAA
  TCATTTGATGAAAAAGAAAATACTGATGACTTATCGACGGATTTAGTTTGGTATTTATGG
  ACTGGGAAATATTCGTCACTATTTGGTAAACGTGCCATGACTACGTTTGAGCGATACTTT
  ATTGCAGATAAAGCTTCTCATAAAGAAGAAAAGAATCCGTACTATCATCTTCGTGAAGAT
  GTGAATATGGTTCGTAAAATGCTCAGTGATTTCGGATTAAATCCAGATGAAGGACGCATT
  ATTAATGGTCACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGA
  AAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCA
  GGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAAC
  GCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGAT
  AAAGAATTACAACGTAAGAAGATTCGTGATACCAATATTGGTAAAGATCTTCAAGCTCAA
  ATAGATATTTTGAAAATGTTAATGCATGATCGTTACTTAGACTAA
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⊟Protein

⊟General

• locus tag: NWMN_RS13880 [old locus tag: NWMN_2414 ]
• symbol: NWMN_RS13880
• description: fructose 1,6-bisphosphatase
• length: 654
• theoretical pI: 6.04366
• theoretical MW: 76174.5
• GRAVY: -0.520336

⊟Function

• reaction:
  EC 3.1.3.11?  ExPASy
  Fructose-bisphosphatase D-fructose 1,6-bisphosphate + H₂O = D-fructose 6-phosphate + phosphate
• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Calcineurin (CL0163) FBPase_2; Firmicute fructose-1,6-bisphosphatase (PF06874; HMM-score: 940.9)
  and 1 more
  no clan defined GP24_25; Tail assembly protein Gp24 and Gp25 (PF17388; HMM-score: 14.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002427
  • TAT(Tat/SPI): 0.000086
  • LIPO(Sec/SPII): 0.000178
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446114313 NCBI
• RefSeq: WP_000192168 NCBI
• UniProt: see NWMN_2414

⊟Protein sequence

• MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGTEHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKLIKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSNEYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIMDTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPLLTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRPNFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLLSFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQELLDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHKEEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVIDGGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQRKKIRDTNIGKDLQAQIDILKMLMHDRYLD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see NWMN_2414

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications