PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS11350 [old locus tag: NWMN_1967 ]
• pan locus tag^?: SAUPAN005311000
• symbol: NWMN_RS11350
• pan gene symbol^?: ilvA
• synonym:
• product: L-threonine dehydratase biosynthetic IlvA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS11350 [old locus tag: NWMN_1967 ]
• symbol: NWMN_RS11350
• product: L-threonine dehydratase biosynthetic IlvA
• replicon: chromosome
• strand: +
• coordinates: 2178912..2180180
• length: 1269
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2178912..2180180) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1967

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGACAGTCAAAACAACAGTTTCTACGAAAGATATCGATGAGGCATTTTTAAGACTTAAA
  GATATTGTCAAAGAAACACCTTTACAATTAGACCATTACTTATCTCAAAAGTATGATTGT
  AAAGTCTATTTAAAACGAGAAGATTTACAATGGGTACGTTCTTTTAAATTAAGAGGTGCT
  TACAACGCTATTTCTGTTTTATCAGATGAAGCTAAAAGTAAAGGTATTACATGTGCAAGT
  GCAGGTAATCATGCTCAAGGTGTTGCCTATACAGCTAAAAAACTTAATTTAAACGCTGTT
  ATCTTTATGCCAGTCACTACACCTTTACAAAAGGTAAATCAAGTAAAGTTCTTTGGAAAT
  AGTAACGTTGAAGTTGTACTCACTGGTGATACATTTGATCACTGTTTAGCTGAAGCTTTA
  ACTTATACAAGTGAACATCAAATGAACTTTATAGATCCATTCAATAATGTTCATACAATT
  TCTGGACAAGGTACGCTTGCTAAAGAAATGCTAGAACAAGCAAAGTCTGACAATGTTAAC
  TTTGATTATCTATTTGCCGCAATTGGTGGTGGCGGTTTAATTTCAGGTATTAGTACTTAC
  TTTAAAACCTATTCACCTACCACGAAAATTATAGGTGTTGAACCTTCAGGTGCAAGTAGT
  ATGTATGAATCTGTTGTGGTAAATAATCAGGTAGTCACATTGCCTAATATCGATAAATTT
  GTGGACGGTGCATCTGTAGCTAGAGTTGGCGATATTACATTTGAAATTGCAAAAGAAAAT
  GTAGATGATTACGTTCAAGTAGATGAAGGTGCAGTTTGTTCTACGATTTTAGATATGTAT
  TCAAAACAAGCAATTGTAGCAGAACCTGCTGGCGCATTAAGTGTAAGTGCGCTTGAAAAC
  TATAAAGATCATATTAAAGGTAAAACAGTGGTTTGTGTCATTAGTGGTGGTAATAATGAT
  ATTAATCGAATGAAAGAAATTGAAGAACGTTCATTACTATACGAAGAAATGAAGCATTAC
  TTTATCTTAAATTTCCCTCAACGTCCAGGTGCATTGAGAGAATTTGTAAATGACGTATTA
  GGACCTCAAGACGATATTACTAAATTTGAATACTTAAAAAAATCTTCTCAAAATACAGGT
  ACTGTCATTATTGGTATTCAACTTAAAGATCATGATGATTTAATACAACTCAAACAACGT
  GTAAATCATTTCGATCCTTCCAATATTTATATTAATGAAAATAAGATGTTATATTCATTG
  TTAATTTAA
  60
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  1269
  

⊟Protein

⊟General

• locus tag: NWMN_RS11350 [old locus tag: NWMN_1967 ]
• symbol: NWMN_RS11350
• description: L-threonine dehydratase biosynthetic IlvA
• length: 422
• theoretical pI: 5.46176
• theoretical MW: 46937.1
• GRAVY: -0.182938

⊟Function

• reaction:
  EC 4.3.1.19?  ExPASy
  Threonine ammonia-lyase L-threonine = 2-oxobutanoate + NH₃
• TIGRFAM:
  Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 615.2)
  and 16 more
  Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 361.8)
  Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 309.1)
  ectoine utilization protein EutB (TIGR02991; HMM-score: 194.2)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 65.3)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 63.4)
  Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 63.1)
  Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 60.5)
  diaminopropionate ammonia-lyase (TIGR03528; EC 4.3.1.15; HMM-score: 59.4)
  Metabolism Energy metabolism Other diaminopropionate ammonia-lyase family (TIGR01747; EC 4.3.1.15; HMM-score: 57.3)
  Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 51.3)
  pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family (TIGR01275; HMM-score: 48.1)
  Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 43.1)
  Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 38.8)
  Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 17.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 16.1)
  Metabolism Energy metabolism Methanogenesis cysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 16.1)
• TheSEED: see NWMN_1967
• PFAM:
  NADP_Rossmann (CL0063) PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 247)
  and 3 more
  ACT (CL0070) Thr_dehydrat_C; C-terminal regulatory domain of Threonine dehydratase (PF00585; HMM-score: 76.8)
  TIM_barrel (CL0036) SOR_SNZ; SOR/SNZ family (PF01680; HMM-score: 14.3)
  PP-binding (CL0314) Ribosomal_L50; Ribosomal subunit 39S (PF10501; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9153
  • Cytoplasmic Membrane Score: 0.0444
  • Cell wall & surface Score: 0.0013
  • Extracellular Score: 0.0391
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003093
  • TAT(Tat/SPI): 0.000251
  • LIPO(Sec/SPII): 0.000423
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446138998 NCBI
• RefSeq: WP_000216853 NCBI
• UniProt: see NWMN_1967

⊟Protein sequence

• MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYLKREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIFMPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTISGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGASSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTILDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLYEEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHDDLIQLKQRVNHFDPSNIYINENKMLYSLLI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see NWMN_1967

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications