From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS09650 [old locus tag: NWMN_1719 ]
  • pan locus tag?: SAUPAN004592000
  • symbol: NWMN_RS09650
  • pan gene symbol?: lukE
  • synonym:
  • product: gamma-hemolysin subunit A

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS09650 [old locus tag: NWMN_1719 ]
  • symbol: NWMN_RS09650
  • product: gamma-hemolysin subunit A
  • replicon: chromosome
  • strand: -
  • coordinates: 1916843..1917778
  • length: 936
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1916843..1917778) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1719

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTTAAGAAAAAAATGTTAGCTGCAACTTTGTCAGTAGGACTGATTGCACCTTTAGCA
    TCTCCGATTCAAGAATCTAGAGCAAATACTAATATTGAAAATATTGGTGATGGTGCTGAA
    GTAATCAAACGTACGGAGGATGTAAGTAGTAAGAAATGGGGCGTTACTCAAAATGTCCAA
    TTCGACTTTGTAAAAGATAAAAAATATAACAAAGACGCTTTAATTGTTAAAATGCAAGGT
    TTTATTAATTCCAGAACTTCATTTTCAGATGTGAAGGGTAGTGGATATGAATTAACTAAA
    CGAATGATTTGGCCATTCCAATATAATATAGGACTGACGACTAAAGATCCAAATGTTAGC
    TTAATCAATTACCTTCCTAAAAACAAAATAGAAACTACTGATGTTGGTCAAACATTAGGA
    TATAACATTGGAGGTAATTTCCAGTCAGCACCATCTATAGGTGGCAATGGCTCATTTAAT
    TATTCTAAAACAATTAGTTATACCCAAAAGAGTTATGTCAGTGAAGTAGACAAGCAAAAC
    TCAAAATCTGTTAAATGGGGTGTTAAAGCAAACGAATTTGTTACGCCTGATGGAAAAAAA
    TCTGCGCATGATAGATATTTATTCGTACAAAGTCCAAATGGTCCAACAGGTTCAGCAAGA
    GAATATTTTGCTCCTGATAATCAATTGCCACCTTTAGTTCAAAGTGGCTTTAATCCATCG
    TTTATCACTACACTATCACATGAAAAAGGTTCAAGTGATACGAGTGAATTTGAAATTTCA
    TATGGTAGAAACTTAGATATTACATATGCGACTTTATTCCCTAGAACTGGTATTTACGCA
    GAAAGAAAGCATAATGCATTTGTAAATAGAAACTTTGTAGTTAGATATGAAGTTAATTGG
    AAAACACACGAAATTAAAGTGAAAGGACATAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS09650 [old locus tag: NWMN_1719 ]
  • symbol: NWMN_RS09650
  • description: gamma-hemolysin subunit A
  • length: 311
  • theoretical pI: 9.98997
  • theoretical MW: 34818.9
  • GRAVY: -0.582315

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance beta-channel forming cytolysin (TIGR01002; HMM-score: 305.4)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 287.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 28 aa
    • SP(Sec/SPI): 0.985549
    • TAT(Tat/SPI): 0.00836
    • LIPO(Sec/SPII): 0.003798
    • Cleavage Site: CS pos: 28-29. SRA-NT. Pr: 0.9060
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKRTEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRMIWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFNYSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGSAREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRTGIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_RS09650&oldid=84887"