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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS09130 [old locus tag: NWMN_1626 ]
- pan locus tag?: SAUPAN004397000
- symbol: NWMN_RS09130
- pan gene symbol?: acsA
- synonym:
- product: acetate--CoA ligase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS09130 [old locus tag: NWMN_1626 ]
- symbol: NWMN_RS09130
- product: acetate--CoA ligase
- replicon: chromosome
- strand: -
- coordinates: 1809347..1811053
- length: 1707
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1681ATGAAAGTCGAAGTTTATAAAGGAGCGCAAGGTAAACATAACCTTAAAGATTATGAAGAA
ACATATAATACTTTTGATTGGAAAGACGTAGAACAAGCATTTTCTTGGAGTGAAACTGGA
AAAATGAACATGGCATATGAATGCATAGATCGCCATGTAGATCAAGGATTAGGGGATAAA
ATAGCGTTAAATTACAAAGATGAGCACAGAAAAGAATCGTATACTTATAAAGATATGCAA
CGGTTATCTAATAAAGCAGCGAATGTTTTGTCTGAACATGCAGAAGTTGACAAAGGTGAC
AGAGTATTTATATTTATGTCGCGTACACCTGAACTATATTTTGCGTTGTTAGGTGTTTTA
AAAATTGGTGCAATTGTTGGGCCGTTATTTGAAGCATTTATGGAAAAGGCAGTTGCGGAT
AGATTAGAGAACAGTGAAGCTAAAGTGTTAATTACTAATAAGGCATTGTTACCTCGAGTA
CCTGTAGATAAATTACCAAACTTGAAAAAAATTGTTGTCGTAGATGAGGATGTAGAAGAC
AATTACATAGACTTCATTAGTTTGATGGAAACTGCTAGCGATGAATTTGACATTGAATGG
TTAAAGTCGGATGATGGTTTGATTTTACATTATACATCAGGTTCTACTGGGCAACCTAAA
GGTGTATTGCATGTTCAACAAGCAATGTTAGTGCACTATATTTCTGGAAAATATGTATTA
GATTTACAAGAAGATGATGTTTATTGGTGTACAGCAGATCCAGGTTGGGTTACAGGAACA
TCTTATGGTATTTTTGCACCATGGTTAAATGGCGCTACAAATTGTATAGCTGGTGGTCGC
TTTTCGCCAGAACAGTGGTATAGTATGATTGAAGATTTTAAAGTGACGATTTGGTATACG
GCACCAACAGCTTTAAGAATGTTAATGAGTGCTGGTGACGATATTGTTGAGAAATATGAC
TTGTCATCGTTACGTTCGATTCTATCAGTAGGTGAGCCTTTAAATCCTGAAGTTATAAAA
TGGGCGAAAAAAGTATACGGTTTAACGGTGTTAGATACTTGGTGGATGACAGAAACAGGT
GGACATATGATTGTTAACTATCCAACGATGGACGTCAAGCTTGGCTCAATGGGCAAACCA
TTACCTGGTATTCAAGCTGCAATTATCGATGATGCAGGGAATGAATTACCACCAAATCGA
ATGGGCAACCTTGCTATAAAAAAAGGCTGGCCATCAATGATGTATCGTATCTGGAAGAAT
CCAGAAAAATATAAATCATATTTTATTGGAGACTGGTATGTATCTGGTGATTCGGCATAT
AAAGATGAAGATGGTTACTTCTGGTTCCAAGGACGTGTTGATGATGTAATTATGACAGCT
GGTGAACGAGTTGGACCATTTGAGGTTGAGTCTAAATTGGTTGAACACGAAGCAGTTGCC
GAAGCAGGAATTATTGGTAAACCTGATCCGGTTCGCGGTGAAATAATTAAGGCGTTTGTT
GCACTGAGAAAAGGATATGAACCAACAGACGAATTAAAAGAAGAAATTCGTATATTTGTT
AAAGAAGGTTTGTCGGCACATGCAGCACCACGTGAAATCGAATTTAAAGATAAATTACCT
AAAACACGGTCAGGTAAAATTATGAGACGTGTATTAAAAGCTTGGGAATTAAATTTAGAT
GCTGGGGATTTAAGTACAATGGAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS09130 [old locus tag: NWMN_1626 ]
- symbol: NWMN_RS09130
- description: acetate--CoA ligase
- length: 568
- theoretical pI: 4.81296
- theoretical MW: 64364.1
- GRAVY: -0.33169
⊟Function[edit | edit source]
- reaction: EC 6.2.1.1? ExPASyAcetate--CoA ligase ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
- TIGRFAM: acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 543.7)and 12 morepropionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 306.2)benzoate-CoA ligase family (TIGR02262; HMM-score: 287.7)cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 199.3)dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 187.8)amino acid adenylation domain (TIGR01733; HMM-score: 176.3)Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 158)acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 157.9)Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 139.5)Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 135.2)Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 97.8)4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 53.4)L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 36.4)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 317.9)and 1 moreAMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 82.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.015848
- TAT(Tat/SPI): 0.000645
- LIPO(Sec/SPII): 0.00221
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNMAYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRVFIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRVPVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQPKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCIAGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPLNPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDAGNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQGRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEPTDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLSTME
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CcpA, CodY see NWMN_1626
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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