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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS08415 [old locus tag: NWMN_1495 ]
- pan locus tag?: SAUPAN004179000
- symbol: NWMN_RS08415
- pan gene symbol?: —
- synonym:
- product: haloacid dehalogenase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS08415 [old locus tag: NWMN_1495 ]
- symbol: NWMN_RS08415
- product: haloacid dehalogenase
- replicon: chromosome
- strand: -
- coordinates: 1662790..1663374
- length: 585
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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541ATGAACATTGAAAAAGCAAAACGGCTTGCAAAAGAGAAACTGCCAGAGAAACGTTATAAT
CATTCTTTAAGAGTTGCAGAAACAGCGATTAAATTGGCAGAAATTTATGATGGAGACACT
AGTAAAGTAGAATTAGCAGGTGTATTACATGATTTCTGTAAATATGATGATTTAGGTAAA
ATGTATCAAATTGTTCGACAATACGAATTAGGTAATGATCTACTGAGTTATGGTAGTGAA
ATATTGCATGGCCCTGTGTGTGCAGCAATCATGGAACATGAATATGGTATCAATGATGAG
GAAGTATTAATGGCTATCAAATACCATACTACTGGACGTCAACAAATGACAAAAACTGAA
AAACTGATTTTTATTGCAGATTACATCGAACCTGGAAGAACAATCCCAGGAGTTGATGAT
ATTCGAGATATGGCATACAATCAAGGTAGTTTAGATAAGACAATTTATGAAATTTCTAAA
CGCACAGTACTATTTTTAATACAGAAAGATATTACGGTATATAATAAGACGATTGACTGT
TTAAATTATTATAACTATAGTGACGAAAGAATAAAGGATGATTAA60
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585
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS08415 [old locus tag: NWMN_1495 ]
- symbol: NWMN_RS08415
- description: haloacid dehalogenase
- length: 194
- theoretical pI: 5.15494
- theoretical MW: 22463.5
- GRAVY: -0.49433
⊟Function[edit | edit source]
- TIGRFAM: Unknown function Enzymes of unknown specificity putative HD superfamily hydrolase (TIGR00488; HMM-score: 140.2)and 4 moreHDIG domain (TIGR00277; HMM-score: 24.5)Hypothetical proteins Conserved TIGR00295 family protein (TIGR00295; HMM-score: 18.9)HD domain protein, cyanamide hydratase family (TIGR03401; EC 4.2.1.-; HMM-score: 16.3)Transcription Degradation of RNA ribonuclease Y (TIGR03319; EC 3.1.-.-; HMM-score: 11.2)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: HD_PDEase (CL0237) HD; HD domain (PF01966; HMM-score: 44)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005836
- TAT(Tat/SPI): 0.000227
- LIPO(Sec/SPII): 0.000722
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVELAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEVLMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISKRTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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