PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_RS08340 [old locus tag: NWMN_1481 ]
pan locus tag^?: SAUPAN004161000
symbol: NWMN_RS08340
pan gene symbol^?: prmA
• synonym:
• product: 50S ribosomal protein L11 methyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS08340 [old locus tag: NWMN_1481 ]
• symbol: NWMN_RS08340
• product: 50S ribosomal protein L11 methyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1647195..1648133
• length: 939
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1647195..1648133) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1481

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAACTGGACAGAGCTTTCAATTATTATTAATCATGAAGCAGTAGAATTGGCTACCAAT
  ATACTTGAAAATCATGGATCAAATGGTGTCGTGATAGAAGATTCAGATGATTTAATTAAC
  CAACCAGAAGATAAATACGGTGAAATTTACGCTTTGAAAAAAGAGGATTATCCAGATAAG
  GGAGTAAGATTGAAAGCCTATTTTAATGAAATGACTTATGATGATAAGTTGCGACAGCAA
  ATTAAAGATGAGTTATTAAATTTAGATGAACTTGATCAACATAACGTTCAATTCAGTGAG
  CAAATTATTGCAGAGACGGATTGGGAAAATGAATGGAAAAACTATTTCCATCCATTCCGA
  GCGTCGAAGAAGTTCACAATAGTTCCTAGTTGGGAAACATATGCTAAAGAAGCGGATGAA
  GAGCTTTGCATTGAGCTCGACCCAGGTATGGCTTTTGGAACAGGTGATCATCCGACTACA
  AGTATGTGTTTGAAGGCAATAGAAACATATGTATTGCCACAGCATTCAGTAATTGATGTT
  GGTACTGGCTCAGGTATATTAAGTATTGCAAGTCATCTAATCGGTGTAAAACGTATTAAA
  GCGTTGGATATTGATGAAATGGCAGTGAGTGTAGCTAAAGAAAACTTCAGAAGAAATCAT
  TGTGAAACGTTAATTGAAGCTGTTCCAGGTAACTTATTGAAAGACGAAACAGAAAAATTT
  GATATTGTAATAGCAAATATTTTAGCGCATATTATTGATGAAATGATTGAAGATGCTTAT
  AATACTCTAAATGAAGGCGGTTATTTTATTACTTCTGGTATTATAAAAGAGAAGTATGAA
  GGTATACAGTCACATATGGAGCGTGTAGGTTTTAAAATTATTTCAGAACAACATGACAAC
  GGTTGGGTTTGTCTTGTTGGCCAGAAAGTGAGTGAATAA
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  939
  

⊟Protein

⊟General

• locus tag: NWMN_RS08340 [old locus tag: NWMN_1481 ]
• symbol: NWMN_RS08340
• description: 50S ribosomal protein L11 methyltransferase
• length: 312
• theoretical pI: 4.33956
• theoretical MW: 35512.7
• GRAVY: -0.376923

⊟Function

• reaction:
  EC 2.1.1.-?  ExPASy
• ⊞TIGRFAM:
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 257.9)
  and 13 more
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 45.4)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 39.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 37.6)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 37.6)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 36.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 32)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 30.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 24.5)
  methyltransferase, ATP-grasp peptide maturase system (TIGR04188; HMM-score: 18.3)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 17)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG (TIGR00138; EC 2.1.1.170; HMM-score: 16.2)
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 15.5)
  Hypothetical proteins Conserved putative methyltransferase, TIGR01177 family (TIGR01177; HMM-score: 14.6)
• TheSEED: see NWMN_1481
• ⊞PFAM:
  NADP_Rossmann (CL0063) PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 362.5)
  and 15 more
  MTS; Methyltransferase small domain (PF05175; HMM-score: 55.7)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 39.3)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 35.7)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 31.8)
  Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 31.5)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 28.6)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 21)
  TRM5-TYW2_MTfase; TRM5/TYW2 methyltransferase domain (PF02475; HMM-score: 20.9)
  Spermine_synth; Spermine/spermidine synthase domain (PF01564; HMM-score: 18.2)
  Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 17.6)
  PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 17.4)
  UPF0020; RMKL-like, methyltransferase domain (PF01170; HMM-score: 13.1)
  GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 13)
  TRM; N2,N2-dimethylguanosine tRNA methyltransferase (PF02005; HMM-score: 12.2)
  RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.998
  • Cytoplasmic Membrane Score: 0.0004
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0014
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007117
  • TAT(Tat/SPI): 0.000217
  • LIPO(Sec/SPII): 0.000972
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447027355 NCBI
• RefSeq: WP_001104611 NCBI
• UniProt: see NWMN_1481

⊟Protein sequence

• MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPEDKYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQIIAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTTSMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRRNHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIKEKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications