PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS07310 [old locus tag: NWMN_1298 ]
• pan locus tag^?: SAUPAN003795000
• symbol: NWMN_RS07310
• pan gene symbol^?: pstA
• synonym:
• product: phosphate ABC transporter, permease protein PstA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS07310 [old locus tag: NWMN_1298 ]
• symbol: NWMN_RS07310
• product: phosphate ABC transporter, permease protein PstA
• replicon: chromosome
• strand: -
• coordinates: 1429282..1430199
• length: 918
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1429282..1430199) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1298

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
  541
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  721
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  841
  901
  
  ATGGAAACGACAGATAATAATAGACAATCACTCGTCGATCAACAACTTGTCCAAAAACAT
  TTATCATCCAGAACGGTTAAAAATAAAGTGTTCAAACTCATATTTTTAGCATGTACATTA
  TTAGGACTTGTCGTACTTATTGCGTTGTTAACTCAAACATTGATTAAAGGGGTAAGTCAT
  TTAAATTTACAGTTTTTCACTAATTTTTCTTCTTCAACACCATCTATGGCTGGCGTTAAA
  GGCGCGTTAATCGGTTCACTTTGGTTAATGTTAAGTATCATTCCATTATCAATCATCCTA
  GGAATAGGTACAGCTATATACTTAGAAGAATATGCGAAAAACAACAAATTTACTCAGTTT
  GTTAAAATCAGTATTTCCAATTTAGCTGGTGTACCATCAGTTGTATTTGGGTTATTAGGT
  TATACTTTGTTCGTTGGTGGTGCAGGGATTGAAGCCTTGAAAATGGGTAACAGTATATTG
  GCAGCAGCGCTAACAATGACCTTACTGATATTACCAATTATTATTGTTTCAAGTCAGGAA
  GCAATTAGAGCTGTACCTAACTCAGTACGCGAAGCTTCTTACGGCTTAGGTGCTAATAAA
  TGGCAAACGATAAGACGTGTTGTCTTACCAGCAGCGTTACCTGGTATTTTAACTGGATTC
  ATTTTGTCTCTTTCAAGAGCACTGGGAGAAACAGCGCCACTTGTGCTAATCGGTATACCG
  ACTATATTATTGGCAACACCTAGAAGTATATTGGATCAATTTTCAGCATTACCTATCCAA
  ATATTTACTTGGGCGAAAATGCCTCAAGAAGAATTCCAGAATGTTGCATCGGCAGGCATT
  ATCGTTTTACTAGTTATCTTAATCTTAATGAATGGCGTTGCGATTATTTTACGTAACAAA
  TTTAGTAAAAAATTCTAA
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  918
  

⊟Protein

⊟General

• locus tag: NWMN_RS07310 [old locus tag: NWMN_1298 ]
• symbol: NWMN_RS07310
• description: phosphate ABC transporter, permease protein PstA
• length: 305
• theoretical pI: 10.7035
• theoretical MW: 32870.1
• GRAVY: 0.751803

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 303.3)
  and 13 more
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 142.3)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 49.9)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 48.5)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 48.4)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein (TIGR00969; HMM-score: 45.1)
  2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 40.5)
  2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 33.4)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysW (TIGR02140; HMM-score: 29.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, permease protein (TIGR03262; HMM-score: 22.6)
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 19.2)
  ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 18.9)
  Metabolism Transport and binding proteins Anions nitrate ABC transporter, permease protein (TIGR01183; HMM-score: 17.3)
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 16.4)
• TheSEED: see NWMN_1298
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 74.7)
  and 2 more
  no clan defined DUF3333; Domain of unknown function (DUF3333) (PF11812; HMM-score: 11.4)
  DUF3671; Fam-L, Fam-M like protein (PF12420; HMM-score: 8.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9999
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0001
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005391
  • TAT(Tat/SPI): 0.000415
  • LIPO(Sec/SPII): 0.011986
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 446372442 NCBI
• RefSeq: WP_000450297 NCBI
• UniProt: see NWMN_1298

⊟Protein sequence

• METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLVVLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGIGTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSILAAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILTGFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVASAGIIVLLVILILMNGVAIILRNKFSKKF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications