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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS06195 [old locus tag: NWMN_1093 ]
  • pan locus tag?: SAUPAN003453000
  • symbol: NWMN_RS06195
  • pan gene symbol?: murD
  • synonym:
  • product: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS06195 [old locus tag: NWMN_1093 ]
  • symbol: NWMN_RS06195
  • product: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1197324..1198673
  • length: 1350
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1197324..1198673) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1093

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCTTAATTATACAGGGTTAGAAAATAAAAATGTATTAGTTGTCGGTTTGGCAAAAAGT
    GGTTATGAAGCAGCTAAATTATTAAGTAAATTAGGTGCGAATGTAACTGTCAATGATGGA
    AAAGACTTATCACAAGATGCTCATGCAAAAGATTTAGAATCTATGGGCATTTCTGTTGTA
    AGTGGAAGTCATCCATTAACGTTGCTTGATAATAATCCAATAATTGTTAAAAATCCTGGA
    ATACCTTATACAGTATCTATTATTGATGAAGCAGTGAAACGAGGTTTGAAAATTTTAACA
    GAAGTTGAGTTAAGTTATCTAATCTCTGAAGCACCAATCATAGCTGTAACGGGTACAAAT
    GGTAAAACGACAGTTACTTCTCTAATTGGAGATATGTTTAAAAAAAGTCGCTTAACTGGA
    AGATTATCCGGCAATATTGGTTATGTTGCATCTAAAGTAGCACAAGAAGTAAAGCCTACA
    GATTATTTAGTTACAGAGTTGTCGTCATTCCAGTTACTTGGAATCGAAAAGTATAAACCA
    CACATTGCTATAATTACTAACATTTATTCGGCGCATCTAGATTACCATGAAAATTTAGAA
    AACTATCAAAATGCTAAAAAGCAAATATATAAAAATCAAACGGAAGAGGATTATTTGATT
    TGTAATTATCATCAAAGACAAGTGATAGAGTCGGAAGAATTAAAAGCTAAGACATTGTAT
    TTCTCAACTCAACAAGAAGTTGATGGTATTTATATTAAAGATGGTTTTATCGTTTATAAA
    GGTGTTCGTATTATTAACACTGAAGATCTAGTATTGCCTGGTGAACATAATTTAGAAAAT
    ATATTAGCAGCTGTGCTTGCTTGTATTTTAGCTGGTGTACCTATTAAAGCAATTATTGAT
    AGTTTAACTACATTTTCAGGAATAGAGCATAGATTGCAATATGTTGGTACTAATAGAACT
    AATAAATATTATAATGATTCCAAAGCAACAAACACGCTAGCAACACAGTTTGCCTTAAAT
    TCATTTAATCAACCAATCATTTGGTTATGTGGTGGTTTGGATCGAGGGAATGAATTTGAC
    GAACTCATTCCTTATATGGAAAATGTTCGCGCGATGGTTGTATTCGGACAAACGAAAGCT
    AAGTTTGCTAAACTAGGTAATAGTCAAGGGAAATCGGTCATTGAAGCGAACAATGTCGAA
    GACGCTGTTGATAAAGTACAAGATATTATAGAACCAAATGATGTTGTATTATTGTCACCT
    GCTTGTGCGAGTTGGGATCAATATAGTACTTTTGAAGAGCGTGGAGAGAAATTTATTGAA
    AGATTCCGTGCCCATTTACCATCTTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1350

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS06195 [old locus tag: NWMN_1093 ]
  • symbol: NWMN_RS06195
  • description: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase
  • length: 449
  • theoretical pI: 5.49171
  • theoretical MW: 49843.4
  • GRAVY: -0.167038

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.2.9?  ExPASy
    UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 415.2)
    and 10 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 131.2)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 86)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 83.6)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 49.6)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 34.3)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 24.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 17.7)
    Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 13.9)
    3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 13.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin siroheme synthase, N-terminal domain (TIGR01470; HMM-score: 12.8)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 117.2)
    and 7 more
    Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 37.9)
    NADP_Rossmann (CL0063) Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 15.7)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 15.4)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 15.2)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 15.2)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 14.6)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019906
    • TAT(Tat/SPI): 0.00131
    • LIPO(Sec/SPII): 0.001645
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLSQDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEVELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPTDYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDYLICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEHNLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLATQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKSVIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_RS0292050S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_RS03640LysR family transcriptional regulator  [1] (data from MRSA252)
    NWMN_RS08930pyruvate kinase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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