PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS05840 [old locus tag: NWMN_1032 ]
• pan locus tag^?: SAUPAN001699000
• symbol: NWMN_RS05840
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS05840 [old locus tag: NWMN_1032 ]
• symbol: NWMN_RS05840
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1133090..1134913
• length: 1824
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1133090..1134913) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1032

⊟Phenotype

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⊟DNA sequence

• 1
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  1801
  
  ATGATAGTAGATAATTTTTCGAAAGACGATAACTTAATCGAGTTACAAACGACATCACAA
  TATAATCCAATTATTGACACAAACATCAGTTTCTATGAATCAGATAGAGGAACTGGTGTT
  TTAAATTTTGCAGTAACTAAGAATAACAGACCGTTATCTATAAGTTCTGAACATGTTAAA
  ACATCTATCGTGTTAAAAACCGATGATTATAACGTAGATAGAGGCGCTTATATTTCAGAC
  GAATTAACGATAGTAGACGCAATTAATGGGCGTTTGCAGTATGTGATACCGAATGAATTT
  TTAAAACATTCAGGCAAGGTGCATGCTCAGGCATTCTTTACACAAAACGGGAGTAATAAT
  GTTGTTGTTGAACGTCAATTTAGCTTCAATATTGAAAATGATTTAGTTAGTGGGTTTGAT
  GGTATAACAAAGCTTGTTTATATCAAATCTATTCAAGATACTATCGAAGCTGTCGGTAAA
  GACTTTAACCAATTAAAGCAAAATATGGCTGATACACAAACGTTAATAGCAAAAGTGAAT
  GATAGTGCGACAAAAGGCATTCAACAAATCGAAATCAAGCAAAACGAAGCTATACAAGCT
  ATTACTGCGACGCAAACTAGTGCAACACAAGCTGTTACAGCTGAATTCGATAAAATAGTT
  GAAAAAGAGCAAGCGATTTTTGAACGTGTTAACGAAGTTGAACAACAAATCAATGGCGCT
  GACCTTGTTAAAGGTAATTCAACAACAAATTGGCAAAAGTCTAAACTTACAGATGATTAC
  GGTAAAGCAATCGAATCGTCTGAGCAGTCCATAGATAGCGTTTTAAGCACAGTTAACACA
  TCTAGGATTATTCATATCACTAGCGCGACAGATGCGCCCTCATTTAAAGATATAGGTACT
  GTCGATACACCTAAAGAAGATGGCGTTGACGATGGTTCAGATATTCCGGTAGCTCCTAAC
  ACTTTAGGAAAATCAGGCGTGTTAGTTGTCTATGTTGTTGATGATAGTACGGCACGTGCA
  ACATGGTATCCAGATGATTCAAACGACGAATATACAAAATATAAAATTAGTGGCACATGG
  TACCCGTTTTATAAAAAAAATGACGGCGATTTAACTAAACAATTCGTCGAAGAAACATCA
  AACAACGCTTTAAATCAAGCCAAGCAGTATGTAGATGATAAATTCGGAACAACGAGTTGG
  CAACAACATAAGTTAACTGAGCCTAACGGCCAATCAATACAAGTTAACTTGAATAATGCA
  CAAGGCGATTTGGGTTATCTAACTGCAGGTAATTACTATGCAACAAGAGTGCCGGATTTA
  CCAAGTGGCGTTGAAAGTTATGAGGGTTATTTATCTGTATTCGTTAAAGACGATACAAAC
  AAGCTATTTAACTTCACGCCTTATAACTCTAAAAAGATTTACACACGATCAATCACAAAC
  GGCAGACTTGAGCAACAGTGGACAGTTCCTAATGAACATAAATCAACGGTATTGTTCGAC
  GGTGGCGCAAATGGTGTAGGTACAACAATCAATCTAACTGAACCGTACACAAACTATTCT
  ATTTTGTTGGTAAGTGGAACTTATCCAGGTGGCGTTATTGAGGGATTCGGACTAACCGCA
  TTACCTAACGCGATTCAATTGAGTAAAGCCAATGTAGTTGACTCAGACGGCAACGGTGGC
  GGTATTTATGAGTGCTTACTATCCAAAACAAGTAGCACTACTTTAAGAATAGATAACGAT
  GTGTACTTTGATTTAGGTAAAACATCAGGCTCTGGAGCGAATGCCAACAAAGTTACTATA
  ACTAAAATTATGGGGTGGAAATAA
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  1824
  

⊟Protein

⊟General

• locus tag: NWMN_RS05840 [old locus tag: NWMN_1032 ]
• symbol: NWMN_RS05840
• description: hypothetical protein
• length: 607
• theoretical pI: 4.50093
• theoretical MW: 66742.3
• GRAVY: -0.460461

⊟Function

• TIGRFAM:
• TheSEED: data available for NCTC8325
• PFAM:
  no clan defined DUF2479; Domain of unknown function (DUF2479) (PF10651; HMM-score: 122)
  and 6 more
  DUF1477; Protein of unknown function (DUF1477) (PF07346; HMM-score: 12.6)
  Collagen_mid; Bacterial collagen, middle region (PF15984; HMM-score: 11.1)
  DUF5314; Family of unknown function (DUF5314) (PF17241; HMM-score: 11)
  DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 10.8)
  SNARE-fusion (CL0445) Syntaxin_2; Syntaxin-like protein (PF14523; HMM-score: 8.8)
  no clan defined DUF5301; Domain of unknown function (DUF5300) (PF17225; HMM-score: 8.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005932
  • TAT(Tat/SPI): 0.000669
  • LIPO(Sec/SPII): 0.001404
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446557171 NCBI
• RefSeq: WP_000634517 NCBI
• UniProt: see NWMN_1032

⊟Protein sequence

• MIVDNFSKDDNLIELQTTSQYNPIIDTNISFYESDRGTGVLNFAVTKNNRPLSISSEHVKTSIVLKTDDYNVDRGAYISDELTIVDAINGRLQYVIPNEFLKHSGKVHAQAFFTQNGSNNVVVERQFSFNIENDLVSGFDGITKLVYIKSIQDTIEAVGKDFNQLKQNMADTQTLIAKVNDSATKGIQQIEIKQNEAIQAITATQTSATQAVTAEFDKIVEKEQAIFERVNEVEQQINGADLVKGNSTTNWQKSKLTDDYGKAIESSEQSIDSVLSTVNTSRIIHITSATDAPSFKDIGTVDTPKEDGVDDGSDIPVAPNTLGKSGVLVVYVVDDSTARATWYPDDSNDEYTKYKISGTWYPFYKKNDGDLTKQFVEETSNNALNQAKQYVDDKFGTTSWQQHKLTEPNGQSIQVNLNNAQGDLGYLTAGNYYATRVPDLPSGVESYEGYLSVFVKDDTNKLFNFTPYNSKKIYTRSITNGRLEQQWTVPNEHKSTVLFDGGANGVGTTINLTEPYTNYSILLVSGTYPGGVIEGFGLTALPNAIQLSKANVVDSDGNGGGIYECLLSKTSSTTLRIDNDVYFDLGKTSGSGANANKVTITKIMGWK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications