PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_RS05770 [old locus tag: NWMN_1018 ]
pan locus tag^?: SAUPAN001685000
symbol: NWMN_RS05770
pan gene symbol^?: —
• synonym:
• product: phage capsid protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS05770 [old locus tag: NWMN_1018 ]
• symbol: NWMN_RS05770
• product: phage capsid protein
• replicon: chromosome
• strand: +
• coordinates: 1120119..1120733
• length: 615
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (1120119..1120733) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_1018

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGATATCCAAGAGAAGTTAAAACTCAAATTACAGTTTTTTGCTGAAGAATCAGATGGA
  GATAATGGAAAATCAAAAGATAACAACGATGATGAAGGCAAAGACAAACAAGACAAAAAG
  ACTAATTCAGAAGAAGAAATCGAAAAAAGACTACAAGAAGAATATAACAAGCGTCTTAAA
  GAAGAATTAAGTCGTCGTATGAAGCAGAAAGAAAAAGAGAAACAAGAAGCTGTTGATGAA
  GCTAAACGATTAGCAAAAATGAACAAAGATCAAATCGCTGAATATGAACGCGAACAAATG
  GAAAAAGAGCTGGAACAATTACGTTCAGAAAAACAATTAAACGAAATGCGTTCAGAAGCA
  CGAAAAATGTTGAGTGAAGCGGAAGTTGATTCATCAGATGAGGTTGTCAATTTGGTTGTA
  ACTGACACTGCTGAACAAACCAAATCGAACGTTGAAGCTTTTTCTAATGCAGTAAAAAAA
  GCGGTTAATGAAGCGGTTAAGGTTAACGCTAGACAATCGCCATTGACTGGTGGAGATTCA
  TTTAATCACTCGACTAAAAATAAACCGCAAAACTTAGCTGAAATAGCTAGACAAAAAAGA
  ATTATTAAAAATTAA
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⊟Protein

⊟General

• locus tag: NWMN_RS05770 [old locus tag: NWMN_1018 ]
• symbol: NWMN_RS05770
• description: phage capsid protein
• length: 204
• theoretical pI: 5.19987
• theoretical MW: 23664.2
• GRAVY: -1.40882

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 2.9)
• TheSEED: see NWMN_1018
• ⊞PFAM:
  no clan defined DUF4355; Capsid assembly scaffolding protein Gp46 (PF14265; HMM-score: 99.6)
  and 3 more
  Peptidase_MA (CL0126) YgjP-like; YgjP-like, metallopeptidase domain (PF01863; HMM-score: 8.5)
  no clan defined Amnionless; Amnionless (PF14828; HMM-score: 8.3)
  NBD_C; Nucleotide-binding C-terminal domain (PF17042; HMM-score: 7.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9909
  • Cytoplasmic Membrane Score: 0.0009
  • Cell wall & surface Score: 0.005
  • Extracellular Score: 0.0032
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008156
  • TAT(Tat/SPI): 0.001648
  • LIPO(Sec/SPII): 0.004845
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446276452 NCBI
• RefSeq: WP_000354307 NCBI
• UniProt: see NWMN_1018

⊟Protein sequence

• MDIQEKLKLKLQFFAEESDGDNGKSKDNNDDEGKDKQDKKTNSEEEIEKRLQEEYNKRLKEELSRRMKQKEKEKQEAVDEAKRLAKMNKDQIAEYEREQMEKELEQLRSEKQLNEMRSEARKMLSEAEVDSSDEVVNLVVTDTAEQTKSNVEAFSNAVKKAVNEAVKVNARQSPLTGGDSFNHSTKNKPQNLAEIARQKRIIKN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications