PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS04545 [old locus tag: NWMN_0803 ]
• pan locus tag^?: SAUPAN003031000
• symbol: NWMN_RS04545
• pan gene symbol^?: dltA
• synonym:
• product: D-alanine--poly(phosphoribitol) ligase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS04545 [old locus tag: NWMN_0803 ]
• symbol: NWMN_RS04545
• product: D-alanine--poly(phosphoribitol) ligase
• replicon: chromosome
• strand: +
• coordinates: 891065..892522
• length: 1458
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (891065..892522) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0803

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACAGATATTATTAACAAGCTGCAAGCGTTTGCGGATGCAAATCCACAAAGCATTGCT
  GTTAGACACACAACTGATGAATTAACTTATCAACAGTTAATGGATGAGTCTAGTAAATTA
  GCACATCGATTACAAGGTAGTAAGAAACCGATGATTTTATTCGGTCACATGTCACCATAT
  ATGATTGTTGGGATGATTGGTGCCATTAAAGCAGGATGTGGATATGTACCTGTAGACACT
  TCAATTCCTGAAGACCGTATTAAAATGATTATTAACAAGGTTCAACCAGAGTTTGTATTT
  AATACGACTGATGAATCATTTGAAAGTTTAGAAGGCGAAGTATTTACAATAGAAGATATT
  AAAACATCTCAAGACCCAGTAATTTTTGATAGTCAGATTAAAGATAACGACACAGTATAC
  ACAATCTTTACATCTGGTTCTACTGGGGAACCTAAAGGTGTTCAAATTGAATATGCAAGT
  TTAGTTCAATTTACTGAGTGGATGTTAGAACTTAATAAATCAGGAAATGAACAACAATGG
  CTTAACCAAGCGCCATTTTCATTTGATTTATCTGTAATGGCTATTTATCCATGTTTAGCA
  TCAGGCGGTACATTAAATCTTGTAGATAAAAACATGATTAATAAACCTAAATTATTAAAT
  GAAATGCTAACAGCAACACCGATTAACATTTGGGTATCAACACCATCATTTATGGAAATG
  TGTTTATTATTACCAACGCTTAATGAAGAACAATATGGTAGTCTTAACGAATTCTTCTTC
  TGTGGTGAAATTCTACCTCACAGAGCAGCAAAAGCGTTAGTAAACCGTTTCCCAAGTGCG
  ACGATTTACAACACATATGGTCCAACTGAAGCTACGGTAGCAGTTACAAGTATTCAAATT
  ACACAAGAAATCTTAGATCAATATCCGACATTACCTGTTGGCGTTGAAAGACCAGGCGCA
  AGATTATCTACTACAGATGAAGGTGAACTTGTTATCGAAGGTCAAAGTGTAAGTTTAGGA
  TACTTAAAAAATGACCAAAAAACAGCTGAAGTATTTAATTTCGATGACGGTATTCGTACA
  TATCACACTGGTGATAAAGCGAAGTTTGAAAATGGTCAATGGTTCATTCAAGGTCGTATT
  GATTTCCAAATCAAATTGAATGGCTACAGAATGGAATTAGAAGAAATTGAAACACAATTA
  CGCCAGTCTGAGTTCGTAAAAGAAGCGATTGTTGTACCTGTATATAAAAATGATAAAGTT
  ATTCATTTAATTGGTGCAATTGTGCCAACGACTGAAGTTACGGACAATGCAGAAATGACT
  AAAAATATTAAAAATGACTTGAAATCACGCTTACCAGAGTATATGATTCCTAGAAAGTTT
  GAATGGATGGAACAATTGCCATTGACTTCAAATGGTAAAATTGACAGAAAGAAAATTGCA
  GAGGTAATTAACGGATGA
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⊟Protein

⊟General

• locus tag: NWMN_RS04545 [old locus tag: NWMN_0803 ]
• symbol: NWMN_RS04545
• description: D-alanine--poly(phosphoribitol) ligase
• length: 485
• theoretical pI: 4.55305
• theoretical MW: 54670.1
• GRAVY: -0.251959

⊟Function

• reaction:
  EC 6.1.1.13?  ExPASy
  D-alanine--poly(phosphoribitol) ligase ATP + D-alanine + poly(ribitol phosphate) = AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)
  EC 6.2.1.-?  ExPASy
  D-alanine + ATP + holo-[D-alanyl-carrier protein] = AMP + diphosphate + D-alanyl-[D-alanyl-carrier protein]?
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 755.6)
  and 12 more
  amino acid adenylation domain (TIGR01733; HMM-score: 322.6)
  acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 160.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 137.3)
  L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 115.2)
  benzoate-CoA ligase family (TIGR02262; HMM-score: 100.1)
  acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 85)
  Metabolism Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 76.9)
  cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 45.4)
  propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 39.6)
  Metabolism Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 38.2)
  dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 35.4)
  4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 33.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 243.5)
  and 2 more
  AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 42.4)
  no clan defined NIR_SIR; Nitrite and sulphite reductase 4Fe-4S domain (PF01077; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004344
  • TAT(Tat/SPI): 0.000288
  • LIPO(Sec/SPII): 0.000819
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446051798 NCBI
• RefSeq: WP_000129653 NCBI
• UniProt: see NWMN_0803

⊟Protein sequence

• MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRLQGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNTTDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYASLVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKLLNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNRFPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEGQSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRMELEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSRLPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications