PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS04135 [old locus tag: NWMN_0730 ]
• pan locus tag^?: SAUPAN002681000
• symbol: NWMN_RS04135
• pan gene symbol^?: —
• synonym:
• product: acetyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS04135 [old locus tag: NWMN_0730 ]
• symbol: NWMN_RS04135
• product: acetyltransferase
• replicon: chromosome
• strand: +
• coordinates: 821721..822206
• length: 486
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (821721..822206) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0730

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  TTGAGGAAATTTTTATCAAAAACACATCATCATACAAACCCTTTATGGCGTGTATACCGT
  CTTGTTAAATTTTCGAAAGTTTTTAAGAATGTAATTATCATTGAATTTTCGAAATTTATT
  CCAAGTATGGTACTGAAAAGACATATATATAAACAACTTTTAAATATTAATATCGGTAAT
  CAATCGTCGATAGCTTATAAAGTAATGTTAGATATTTTTTACCCAGAACTGATTACGATT
  GGTAGTAACAGTGTTATTGGTTACAATGTAACAATTTTGACGCATGAAGCATTAGTTGAT
  GAATTTCGTTATGGACCAGTGACGATAGGATCTAACACTTTGATTGGTGCAAATGCTACC
  ATTTTACCCGGTATAACGATTGGTGACAATGTAAAAGTTGCAGCTGGTACGGTTGTTTCA
  AAAGATATACCGGATAATGGATTTGCATATGGCAACCCTATGTATATAAAAATGATTAGG
  AGGTGA
  60
  120
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  486
  

⊟Protein

⊟General

• locus tag: NWMN_RS04135 [old locus tag: NWMN_0730 ]
• symbol: NWMN_RS04135
• description: acetyltransferase
• length: 161
• theoretical pI: 10.4217
• theoretical MW: 18186.3
• GRAVY: 0.165839

⊟Function

• TIGRFAM:
  sugar O-acyltransferase, sialic acid O-acetyltransferase NeuD family (TIGR03570; HMM-score: 58.6)
  Metabolism Amino acid biosynthesis Serine family serine O-acetyltransferase (TIGR01172; EC 2.3.1.30; HMM-score: 49.7)
  and 15 more
  2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (TIGR03532; EC 2.3.1.89; HMM-score: 46.2)
  colanic acid biosynthesis acetyltransferase WcaB (TIGR04016; EC 2.3.1.-; HMM-score: 45.7)
  non-ribosomal peptide synthetase terminal domain of unknown function (TIGR02353; HMM-score: 42.6)
  phosphonate metabolim protein, transferase hexapeptide repeat family (TIGR03308; HMM-score: 38.4)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase LpxD (TIGR01853; EC 2.3.1.191; HMM-score: 33.9)
  colanic acid biosynthesis acetyltransferase WcaF (TIGR04008; EC 2.3.1.-; HMM-score: 32.9)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 29.9)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 29.9)
  Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 29.9)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase (TIGR01852; EC 2.3.1.129; HMM-score: 19.3)
  UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03992; EC 2.3.1.157,2.7.7.23; HMM-score: 18)
  Metabolism Energy metabolism Other phenylacetic acid degradation protein PaaY (TIGR02287; HMM-score: 16.4)
  2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (TIGR03535; EC 2.3.1.117; HMM-score: 15.7)
  2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (TIGR03536; EC 2.3.1.117; HMM-score: 13.9)
  glucose-1-phosphate thymidylyltransferase (TIGR01208; EC 2.7.7.24; HMM-score: 12.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  HEXAPEP (CL0536) Hexapep_2; Hexapeptide repeat of succinyl-transferase (PF14602; HMM-score: 54)
  Hexapep; Bacterial transferase hexapeptide (six repeats) (PF00132; HMM-score: 49.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006067
  • TAT(Tat/SPI): 0.000113
  • LIPO(Sec/SPII): 0.000361
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447147537 NCBI
• RefSeq: WP_001224793 NCBI
• UniProt: see NWMN_0730

⊟Protein sequence

• MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMVLKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEFRYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIRR

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications