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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS03950 [old locus tag: NWMN_0698 ]
- pan locus tag?: SAUPAN002627000
- symbol: NWMN_RS03950
- pan gene symbol?: —
- synonym:
- product: EamA/RhaT family transporter
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS03950 [old locus tag: NWMN_0698 ]
- symbol: NWMN_RS03950
- product: EamA/RhaT family transporter
- replicon: chromosome
- strand: -
- coordinates: 782596..783462
- length: 867
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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841TTGAATCCTAAAGTAAAAGGTATCATTGCCATTTTAATTTCGGCTATTGGTTTCAGTTTT
ATGTCAGTGTTTTTCAGACTTGCAGGTGACCTTCCTGTTTTTCAAAAATCATTAGCACGA
AACTTAGTAGCAATGTTTATTCCATTATTTTTTATATATAAATACCATCAGCCAATGTTT
GGCAGACTTTCAAGTCAACCCTTATTAATAACACGATCAACACTTGGACTTATTGGTGTA
TTGTTAAATATTTATGCCATTGATCATATGGTACTTAGTGATGCCGATTCATTAATGAAA
CTCAATCCTTTCTGGACGATTTTATTAAGCATTGTTTTCTTACATGAAAAGGTAAGGAAG
TATCAAATTACAGCAATGATAATTGCTATTTTAGGTATGCTTTTAATAGTGAAACCTGAA
TTTTCATCATCTATGATTCCATCATTAGCTGGCTTATTCTCAGGTATTTTTGCAGCATCA
GCATATACATGTGTTCGAGCACTTAGTACAAGAGAAGCACCATATACTATAGTATTTTAC
TTCTCCTTATTTTCTGTCATCGTTTTAATACCTTTCACTGCATATACTTATGAACCAATG
AGTCAGATGCAAATACTTTATCTATTAGGGGCTGGCTTAGCTGCCGCTGTGGGTCAAATT
GGTGTTACTTTAGCATATAGTTTTGCAGCAGCAAAAGATATTTCTATTTTTACGTATGCA
TCTATAATTTTCACAGCAATACTTGGATTTATTCTATTCGGAGAATCTCCAGATTTCTAT
GCAACATTAGGTTACGTCGTTATTATCGGTGCAAGTTATTACATGTTTGAAAAAGCACGC
CGAGACGCCAAAATAATAAAAAAATAA60
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867
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS03950 [old locus tag: NWMN_0698 ]
- symbol: NWMN_RS03950
- description: EamA/RhaT family transporter
- length: 288
- theoretical pI: 9.85057
- theoretical MW: 31954.1
- GRAVY: 0.866667
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Amino acids, peptides and amines carboxylate/amino acid/amine transporter (TIGR00950; HMM-score: 40.1)and 2 moreTransport and binding proteins Unknown substrate protein RarD (TIGR00688; HMM-score: 19.7)phosphonate utilization associated putative membrane protein (TIGR03340; HMM-score: 14.2)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: DMT (CL0184) EamA; EamA-like transporter family (PF00892; HMM-score: 82.8)and 2 moreTPT; Triose-phosphate Transporter family (PF03151; HMM-score: 21.9)Mg_trans_NIPA; Magnesium transporter NIPA (PF05653; HMM-score: 20.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 8
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.030589
- TAT(Tat/SPI): 0.001735
- LIPO(Sec/SPII): 0.009224
- predicted transmembrane helices (TMHMM): 10
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVAMFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLNPFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAASAYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVGQIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMFEKARRDAKIIKK
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: LexA see NWMN_0698
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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