PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS03095 [old locus tag: NWMN_0538 ]
• pan locus tag^?: SAUPAN002352000
• symbol: NWMN_RS03095
• pan gene symbol^?: vraA
• synonym:
• product: long-chain-fatty-acid--CoA ligase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS03095 [old locus tag: NWMN_0538 ]
• symbol: NWMN_RS03095
• product: long-chain-fatty-acid--CoA ligase
• replicon: chromosome
• strand: +
• coordinates: 623637..625013
• length: 1377
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (623637..625013) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_0538

⊟Phenotype

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⊟DNA sequence

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  ATGAACGTAATTTTAGAACAGTTGAAAACACATACTCAAAATAAACCTAATGACATAGCA
  TTACATATCGATGATGAAACAATTACATATAGTCAACTAAATGCCCGCATCACTAGCGCA
  GTTGAATCTTTGCAGAAATATTCACTTAACCCTGTCGTTGCTATTAATATGAAATCACCG
  GTGCAAAGTATTATTTGTTATTTAGCTTTGCATCGTTTACATAAAGTGCCTATGATGATG
  GAAGGTAAATGGCAAAGTACTATACATCGTCAATTGATTGAAAAATATGGTATTAAAGAT
  GTAATTGGAGATACAGGTCTCATGCAGAATATAGACTCACCGATGTTTATTGATTCAACG
  CAATTACAGCACTACCCCAATTTATTACATATTGGTTTTACTTCAGGGACAACTGGACTG
  CCAAAAGCATATTATCGTGATGAAGATTCATGGTTGGCTTCTTTTGAAGTTAATGAAATG
  TTGATGTTAAAAAATGAAAATGCAATAGCAGCCCCTGGACCACTATCGCACTCGTTAACA
  TTATATGCGTTATTGTTTGCTTTAAGTTCCGGTCGTACTTTTATAGGACAGACCACTTTT
  CATCCTGAAAAGTTACTTAATCAATGTCATAAAATATCATCATACAAAGTTGCTATGTTT
  CTTGTTCCAACGATGATTAAATCATTATTGTTAGTTTACAACAATGAACATACAATCCAA
  TCATTTTTTAGCAGTGGAGATAAGCTGCATTCTTCTATTTTTAAAAAGATAAAAAATCAA
  GCAAATGACATAAATTTGATTGAATTTTTTGGTACATCGGAAACCAGTTTTATCAGCTAT
  AACTTGAATCAGCAAGCACCAGTTGAATCAGTAGGTGTGCTATTTCCAAATGTGGAATTG
  AAAACAACGAATCACGATCACAATGGTATAGGAACTATTTGTATAAAAAGTAATATGATG
  TTTAGTGGCTATGTAAGTGAACAATGTATAAATAATGATGAATGGTTTGTTACTAATGAT
  AATGGCTATGTAAAAGAGCAGTATTTATATTTAACGGGACGTCAACAGGATATGTTAATT
  ATTGGTGGTCAAAATATATATCCAGCACATGTTGAACGCCTTTTAACGCAATCTTCGAGC
  ATTGATGAAGCAATTATCATCGGTATTCCAAATGAGCGTTTTGGTCAAATAGGCGTATTG
  CTTTATTCTGGTGATGTGACACTTACACATAAAAATGTAAAACAATTTTTAAAAAAGAAA
  GTGAAACGCTATGAAATTCCATCGATGATTCATCATGTAGAAAAGATGTATTACACTGCA
  AGTGGTAAAATTGCTAGAGAAAAAATGATGTCGATGTATTTGAGAGGTGAATTATAA
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⊟Protein

⊟General

• locus tag: NWMN_RS03095 [old locus tag: NWMN_0538 ]
• symbol: NWMN_RS03095
• description: long-chain-fatty-acid--CoA ligase
• length: 458
• theoretical pI: 7.53263
• theoretical MW: 52023.6
• GRAVY: -0.186245

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone O-succinylbenzoate-CoA ligase (TIGR01923; EC 6.2.1.26; HMM-score: 171.1)
  and 13 more
  acyl-CoA ligase (AMP-forming), exosortase A-associated (TIGR03098; HMM-score: 85.6)
  amino acid adenylation domain (TIGR01733; HMM-score: 78.7)
  cyclohexanecarboxylate-CoA ligase (TIGR03208; EC 6.2.1.-; HMM-score: 75.7)
  dicarboxylate--CoA ligase PimA (TIGR03205; EC 6.2.1.23; HMM-score: 69.1)
  Metabolism Transport and binding proteins Cations and iron carrying compounds (2,3-dihydroxybenzoyl)adenylate synthase (TIGR02275; EC 2.7.7.58; HMM-score: 52.2)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--poly(phosphoribitol) ligase, subunit 1 (TIGR01734; EC 6.1.1.13; HMM-score: 51.8)
  acetate--CoA ligase (TIGR02188; EC 6.2.1.1; HMM-score: 47.4)
  benzoate-CoA ligase family (TIGR02262; HMM-score: 46.8)
  propionate--CoA ligase (TIGR02316; EC 6.2.1.17; HMM-score: 22.9)
  Metabolism Energy metabolism Other phenylacetate-CoA ligase (TIGR02155; EC 6.2.1.30; HMM-score: 19.5)
  4-coumarate--CoA ligase (TIGR02372; EC 6.2.1.12; HMM-score: 17.3)
  L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 15.8)
  Metabolism Central intermediary metabolism Other acetoacetate-CoA ligase (TIGR01217; EC 6.2.1.16; HMM-score: 11.3)
• TheSEED: see NWMN_0538
• PFAM:
  ANL (CL0378) AMP-binding; AMP-binding enzyme (PF00501; HMM-score: 103.8)
  and 3 more
  AMP-binding_C (CL0531) AMP-binding_C; AMP-binding enzyme C-terminal domain (PF13193; HMM-score: 29.8)
  POZ (CL0033) BTB; BTB/POZ domain (PF00651; HMM-score: 15.7)
  Pec_lyase-like (CL0268) DUF4097; Toastrack DUF4097 (PF13349; HMM-score: 13.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8317
  • Cytoplasmic Membrane Score: 0.1607
  • Cell wall & surface Score: 0.0006
  • Extracellular Score: 0.007
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005925
  • TAT(Tat/SPI): 0.000246
  • LIPO(Sec/SPII): 0.000672
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447023579 NCBI
• RefSeq: WP_001100835 NCBI
• UniProt: see NWMN_0538

⊟Protein sequence

• MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESLQKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVIGDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEMLMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVAMFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNERFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMMSMYLRGEL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for JSNZ, N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications