Jump to navigation
Jump to search
NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_2515 [new locus tag: NWMN_RS14440 ]
- pan locus tag?: SAUPAN006322000
- symbol: NWMN_2515
- pan gene symbol?: nrdD
- synonym:
- product: anaerobic ribonucleoside triphosphate reductase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_2515 [new locus tag: NWMN_RS14440 ]
- symbol: NWMN_2515
- product: anaerobic ribonucleoside triphosphate reductase
- replicon: chromosome
- strand: -
- coordinates: 2763498..2765348
- length: 1851
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331566 NCBI
- RefSeq: YP_001333549 NCBI
- BioCyc:
- MicrobesOnline: 3708117 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801ATGAATCAAATCGAAGAAGCATTAACGGGTTTGATTTCTAAAGATCCTGCTATTGTTAAC
GAAAATGCTAACAAAGATAGTGATACATTTTCAACAATGAGAGATTTAACAGCAGGTATC
GTTTCTAAATCTTACGCATTAAATCATTTATTACCAAAGCACGTTGCAGATGCACATCAA
AGAGGGGACATACATTTTCACGACTTAGATTATCATCCATTCCAACCGTTAACTAACTGT
TGTTTAATAGATGCTAAAAATATGCTACATAATGGATTTGAAATAGGCAACGCGAATGTA
ACTTCACCAAAATCAATACAAACTGCATCAGCGCAGCTTGTACAAATTATAGCCAATGTT
TCTAGCAGTCAATATGGTGGCTGTACGGTTGACCGCGTTGACGAATTACTTAGTACATAT
GCACGACATAATGAAGAACAACATAGGAATATCGCAAAGCAATTTGTCAAAGAATCTGAA
ATTGATCGTTATGTTGATCAACAAGTCACTAAAGACATCAATGATGCGATTGAAAGTTTA
GAATATGAAATTAATACCTTATATACATCTAATGGACAGACACCTTTTGTAACATTAGGA
TTCGGCTTAGGTACAGATCATTTAAGTCGCAAAATTCAACAAGCTATCTTAAATACTCGT
ATCAAAGGCTTAGGAAAAGACCGCACGACAGCGATTTTCCCGAAACTTGTATTTTCAATT
AAAAAAGGAACCAACTTTAGTCCGCAAGATCCGAACTATGACATTAAACAACTAGCATTA
AAGTGTTCAACGAAACGTATGTATCCAGATATTTTAAATTATGACAAACTCGTAGAAATA
TTAGGTGATTTCAAAGCGCCAATGGGTTGTCGTTCATTTTTACCAAGTTGGAAAGATGCG
GAAGGTCATTTTGAAAATAATGGTCGTTGTAATCTTGGTGTTGTTACACTTAATTTACCT
AGAATGGCATTAGAATCTGCCGGTAATATGACGAAATTCTGGGAAATCTTTTATGAACGT
ATCGATGTGTTACATGATGCATTACTTTATCGTATAAATCGTTTGAAAGATGCTGTACCG
AATAACGCACCGATTTTATATAAAAGTGGCGCATTTAACTATAAATTAAAAGAAACAGAT
GATGTTGCTGAGTTATTTAAAAATAAACGTGCAACGATTTCAATGGGCTATATAGGGTTG
TATGAAACAGCTACTGTTTTCTATGGTCCAGACTGGGAAACATCTCAAGAAGCAAAAGCA
TTTACGCTTGAAATTCTTAAAGAAATGAAACGTTATCAAACGAAATGGACAGAATTATAT
GACATTTGGTTCAGTATTTACAGTACGCCGAGTGAATCGCTAACGGATCGTTTTTGTCGT
TTAGACCAAGAGAGATTTGGAGATATTAAAGACATTACAGATAAAGGATATTATCAAAAC
TCTTTCCATTATGATGTACGTAAAGATGTTACACCTTTTGAAAAGTTAGATTTTGAAAAA
GATTATCCTTATTATGCGAGTGGTGGTTTCATTCACTATTGTGAGTATCCGAAATTGCAA
CACAATTTGAAAGCACTAGAAGCGGTATGGGACTACTCTTATGACAAAGTTGGTTACTTA
GGTACAAATATTCCGATTGATCATTGTTATGAATGTGATTACGATGGAGATTTTGAAGCA
ACTGAAAAAGGATTTAAATGCCCGAACTGTGGCAATGATAATCCTAAAACAGTTGATGTC
GTTAAACGAACATGTGGTTACCTAGGCAATCCAGTTCAACGTCCAGTAATTAAAGGCCGT
CATAAAGAAATTTGCGCACGAGTAAAACATATGAAAGCGCCTAAAGAATGA60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
1020
1080
1140
1200
1260
1320
1380
1440
1500
1560
1620
1680
1740
1800
1851
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_2515 [new locus tag: NWMN_RS14440 ]
- symbol: NWMN_2515
- description: anaerobic ribonucleoside triphosphate reductase
- length: 616
- theoretical pI: 6.50952
- theoretical MW: 70479.2
- GRAVY: -0.541396
⊟Function[edit | edit source]
- reaction: EC 1.17.4.2? ExPASyRibonucleoside-triphosphate reductase (thioredoxin) 2'-deoxyribonucleoside triphosphate + thioredoxin disulfide + H2O = ribonucleoside triphosphate + thioredoxin
- TIGRFAM: Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase (TIGR02487; EC 1.17.4.2; HMM-score: 576.5)and 3 morePurines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase (TIGR02827; EC 1.17.4.2; HMM-score: 64.4)Transcription Transcription factors transcription factor E (TIGR00373; HMM-score: 13.5)Protein fate Protein folding and stabilization chaperone protein DnaK (TIGR02350; HMM-score: 10.6)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: PFL-like (CL0339) NRDD; Anaerobic ribonucleoside-triphosphate reductase (PF13597; HMM-score: 589.7)and 4 moreGly_radical; Glycine radical (PF01228; HMM-score: 88.9)Phosphatase (CL0031) NleF_casp_inhib; NleF caspase inhibitor (PF16809; HMM-score: 17)Zn_Beta_Ribbon (CL0167) zinc_ribbon_2; zinc-ribbon domain (PF13240; HMM-score: 13.1)zf-FPG_IleRS; Zinc finger found in FPG and IleRS (PF06827; HMM-score: 13)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005243
- TAT(Tat/SPI): 0.005933
- LIPO(Sec/SPII): 0.000678
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKSYALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTSPKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESEIDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILNTRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDKLVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFWEIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRATISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTPSESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASGGFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFKCPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: NrdR (repression) regulon
NrdR (TF) important in Deoxyribonucleotide biosynthesis; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.