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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2466 [new locus tag: NWMN_RS14165 ]
  • pan locus tag?: SAUPAN006230000
  • symbol: oatA
  • pan gene symbol?: —
  • synonym:
  • product: secretory antigen precursor SsaA-like protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2466 [new locus tag: NWMN_RS14165 ]
  • symbol: oatA
  • product: secretory antigen precursor SsaA-like protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2713952..2714719
  • length: 768
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAAAAAATCGTTACAGCTACAATCGCTACAGCAGGACTTGCCACTATCGCATTTGCA
    GGACATGATGCACAAGCCGCAGAACAAAATAACAATGGATATAATTCTAATGACGCTCAA
    TCATACAGCTATACGTATACAATTGATGCACAAGGTAATTATCATTACACTTGGACAGGA
    AATTGGAATCCAAGTCAATTAACGCAAAACAACACATACTACTACAACAACTACAATACT
    TATAGTTATAACAATGCATCTTACAATAACTACTATAATCATTCATATCAATACAATAAC
    TATACAAACAATAGTCAAACAGCAACAAATAACTATTATACTGGTGGTTCAGGTGCAAGT
    TATAGCACAACAAGTAATAATGTTCATGTGACTACAACTGCAGCGCCATCTTCAAATGGT
    CGTTCAATTTCTAATGGTTATGCATCAGGAAGTAACTTATATACTTCAGGACAATGTACT
    TATTATGTATTTGATCGTGTTGGTGGGAAAATTGGTTCAACATGGGGTAACGCAAGTAAT
    TGGGCTAACGCAGCTGCATCATCTGGCTATACAGTGAACAATACACCAAAAGTTGGTGCT
    ATCATGCAAACAACACAAGGCTATTACGGTCATGTTGCTTACGTTGAAGGCGTTAACAGC
    AACGGTTCTGTTCGTGTTTCAGAAATGAACTATGGACATGGTGCTGGTGTGGTTACGTCT
    CGTACAATTTCAGCAAACCAAGCAGGTTCATATAATTTCATTCATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    768

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2466 [new locus tag: NWMN_RS14165 ]
  • symbol: OatA
  • description: secretory antigen precursor SsaA-like protein
  • length: 255
  • theoretical pI: 8.25463
  • theoretical MW: 27589
  • GRAVY: -0.728235

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 53.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: HDAQAAEQ
  • SignalP: Signal peptide SP(Sec/SPI) length 26 aa
    • SP(Sec/SPI): 0.998563
    • TAT(Tat/SPI): 0.000661
    • LIPO(Sec/SPII): 0.000489
    • Cleavage Site: CS pos: 26-27. AQA-AE. Pr: 0.9791
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSYTYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYTNNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGVNSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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