PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2345 [new locus tag: NWMN_RS13510 ]
• pan locus tag^?: SAUPAN005998000
• symbol: opuCC
• pan gene symbol^?: opuCC
• synonym:
• product: glycine betaine/carnitine/choline ABC transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2345 [new locus tag: NWMN_RS13510 ]
• symbol: opuCC
• product: glycine betaine/carnitine/choline ABC transporter
• replicon: chromosome
• strand: -
• coordinates: 2579248..2580189
• length: 942
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332230 NCBI
• RefSeq: YP_001333379 NCBI
• BioCyc:
• MicrobesOnline: 3707947 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAGAAAATTAAATATATACTTGTCGTGTTTGTCTTATCGCTTACCGTATTATCTGGA
  TGTAGTTTGCCCGGACTAGGTAGTAAGAGCACGAAAAATGATGTCAAAATTACAGCATTA
  TCAACAAGCGAATCGCAAATTATTTCACATATGTTACGGTTGTTAATAGAGCATGATACA
  CACGGTAAGATAAAGCCAACATTAGTAAATAATTTAGGGTCAAGTACGATTCAACATAAT
  GCCTTAATTAATGGGGATGCTAATATATCAGGTGTTAGATATAATGGCACAGATTTAACG
  GGAGCTTTGAAGGAAGCACCAATTAAAAATCCTAAGAAAGCAATGATAGCAACACAACAA
  GGATTTAAAAAGAAATTTGATCAAACGTTTTTTGATTCGTATGGTTTTGCGAATACGTAT
  GCATTCATGGTAACGAAGGAAACCGCTAAAAAATATCATTTAGAGACAGTTTCAGATTTA
  GCAAAGCATAGTAAAGATTTACGTTTAGGTATGGATAGTTCATGGATGAATCGTAAAGGC
  GATGGCTATGAAGGATTTAAAAAAGAGTATGGTTTTGACTTTGGTACAGTGAGACCAATG
  CAAATAGGTCTAGTCTACGACGCATTAAACTCAGAGAAGTTAGACGTTGCATTAGGTTAT
  TCTACAGATGGTCGAATTGCGGCGTATGATTTGAAAGTACTTAAAGATGATAAACAATTT
  TTCCCACCTTATGCTGCGAGTGCTGTTGCAACAAATGAATTATTACGGCAACACCCAGAA
  CTTAAAACGACGATTAATAAGTTGACAGGAAAGATTTCGACTTCAGAGATGCAACGCTTG
  AATTATGAAGCGGATGGTAAAGGTAAAGAACCTGCTGTCGTCGCAGAAGAATTTTTGAAA
  AAACACCACTATTTTGATAAACAGAAAGGTGGTCATAAGTAA
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⊟Protein

⊟General

• locus tag: NWMN_2345 [new locus tag: NWMN_RS13510 ]
• symbol: OpuCC
• description: glycine betaine/carnitine/choline ABC transporter
• length: 313
• theoretical pI: 9.89386
• theoretical MW: 34812.7
• GRAVY: -0.4377

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions Protein interactions Phr family secreted Rap phosphatase inhibitor (TIGR04429; HMM-score: 14.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PBP (CL0177) OpuAC; Substrate binding domain of ABC-type glycine betaine transport system (PF04069; HMM-score: 180)
  and 2 more
  LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 12.9)
  no clan defined Nrap_D2; Nrap protein PAP/OAS-like domain (PF17403; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.68
  • Cellwall Score: 0.17
  • Extracellular Score: 0.16
  • Internal Helix: 1
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: TVLSGCS
• SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
  • SP(Sec/SPI): 0.001304
  • TAT(Tat/SPI): 0.000196
  • LIPO(Sec/SPII): 0.998173
  • Cleavage Site: CS pos: 20-21. LSG-CS. Pr: 0.9989
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151222557 NCBI
• RefSeq: YP_001333379 NCBI
• UniProt: A0A0H3KAC9 UniProt

⊟Protein sequence

• MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSESQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGALKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDLAKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVALGYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSEMQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: opuCD < opuCC < opuCB < opuCA

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications