PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1978 [new locus tag: NWMN_RS11445 ]
• pan locus tag^?: SAUPAN005344000
• symbol: NWMN_1978
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1978 [new locus tag: NWMN_RS11445 ]
• symbol: NWMN_1978
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2195267..2196850
• length: 1584
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331215 NCBI
• RefSeq: YP_001333012 NCBI
• BioCyc:
• MicrobesOnline: 3707571 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1561
  
  ATGTCTGAACCTCAAAAATTACATCCGATTTCATATTTTAATGGCATAATCAATGCTATC
  AAACAAAACATAGTCGTCTTTTTTATTTTCATTATCTTTCAATTGAAAGATTTTGATTAT
  ACGAATCCCGAATCATATTTATGGATAGGTATTGTATTTGTATTCTTTTTAATATCATAC
  ATTTCTCAAATTGTCGGAATTATGAACACGCGATATTGGATTGAGGATAATTATTTCATT
  TTAACAACAGGTATTTTTAATAAAAAGCGTAAAGAATTAAATATAAAACGTATTCAGTCC
  GTTGATATGACACAAGGGGTCGTCAATCAAATTATTGGTGGTGTGGATTTACAAATCAAA
  ACACCAAGTGATGGTATTGTCTTAAGTGTTATTTCGAAAAAACAAGGTGAATATCTTGAA
  CGATATATTGATCAATTGCAAACAGAGTTGAAGACAGAAGTGGCGCAAAAGTCTAGTGCT
  GAGAAGAATGACAACTGTAATCATGACATTGAAGGTAATGTGGAAGACATCGCAGAAGGT
  CGCTTAGATGAAGTGCAGAACACTGATAGTATGCTTAATCAGAAAAATAAACGCATTCCT
  ATATATCAGATGAATTTTAAAGAATCACTATTTATGGCAATGACCAGTGGCGCCATTGGT
  GTAACGTTAGCAGCTTTAGTACCGATTTACGGGGCTGTAAGAGAGTTGATCCCATGGTCG
  AAGCTAAATCATGAAATTAGTCAATGGGTTCAAATTGTAAGTATTTCAGTATTAATCATC
  ATTTTACTAGTATTAATAGTGGAATATATTATTGGAACGATTATAACAATGATAAGATTT
  TATGGCTTTTCGGTGATGCTTGAAAATGAGCAACTTAAAATTGAATATGGTCTCTTTAAC
  AAGAAGAAAGTAACTGTACCAACGAAACGATTGCAAGCTGTTGTTGAACGTCAGTCATAT
  ATACGAAGATTATTTGGCTACACAGCAATACATTTTATCATCACAAGTGATTTTGAAAAT
  AAAAAAATCGAAGATGATGACAATGATTCAGGTAGTGTGCCGATATTGCCGTTTATTAAA
  CGAGACAAAGCATATCAAATTATCCAGAATTTAGTACCAGATTTATCTTATCAACAAGTT
  AATGAAGGTATGCCTTTGTCAGGTTTCCACAGACACTTTTTAATACCTAGTATTATTGTC
  ATAGCTATGGCAGGTATAGGCTGTTATTTTTGGAATCAATTATTATTGTTATTTACGATT
  ATAACAATAGTCATTATTGGTTTATTTGTGATCAAAGGATATCTTTATGTCAAACATGCG
  GGTTTTAAAATGACAGAAGAACAAATTACTATCCAGAATTTCGAGCTATTGAAACGAAAT
  ATTTATTATTTTAAAGCGCATCACATTATAGGATTTGACACTTGGCAACATACATTACTT
  AAACGCAACAATTTAAATAATTTTAGCTTTATTGTTGCTAAAGGTGTGGGAGCCCAATCG
  ATTAACTTAAAATACGCAAGAAGTGAAGATGCAAATAAATTGAAATCATGGTATTTAAGA
  GGTGAAAATGATGAGGGATTATAA
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  1584
  

⊟Protein

⊟General

• locus tag: NWMN_1978 [new locus tag: NWMN_RS11445 ]
• symbol: NWMN_1978
• description: hypothetical protein
• length: 527
• theoretical pI: 8.46936
• theoretical MW: 60803.3
• GRAVY: 0.071537

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PH (CL0266) bPH_2; Bacterial PH domain (PF03703; HMM-score: 98.4)
  and 3 more
  bPH_4; Bacterial PH domain (PF06713; HMM-score: 17.2)
  no clan defined MgtE; Divalent cation transporter (PF01769; HMM-score: 11.1)
  DUF2970; Protein of unknown function (DUF2970) (PF11174; HMM-score: 10.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000916
  • TAT(Tat/SPI): 0.000076
  • LIPO(Sec/SPII): 0.004484
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 151222190 NCBI
• RefSeq: YP_001333012 NCBI
• UniProt: A0A0H3KHX0 UniProt

⊟Protein sequence

• MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPESYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVDMTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSAEKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGAIGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVEYIIGTIITMIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFIITSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRHFLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQITIQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARSEDANKLKSWYLRGENDEGL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: alr < acpS < NWMN_1977 < NWMN_1978 < NWMN_1979

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications