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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1453 [new locus tag: NWMN_RS08200 ]
  • pan locus tag?: SAUPAN004131000
  • symbol: NWMN_1453
  • pan gene symbol?: —
  • synonym:
  • product: 5-formyltetrahydrofolate cyclo-ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1453 [new locus tag: NWMN_RS08200 ]
  • symbol: NWMN_1453
  • product: 5-formyltetrahydrofolate cyclo-ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1620732..1621325
  • length: 594
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACATGTATTGTTATTAACGATATAATAGAATTGAATAAAGTGGGTGATAATGTGACT
    AAAAATGAGATTAGAAAATACATTTTACATAAAATGAAGAATTTTAATAAAGCTGAAAAG
    CGAAAAGCAGACACATGGTTAAGAAATCAATTTTTTGCAACTGAAGAATACAAAGAAGCA
    AACGCAATTGCGCTAGTTCTTTCTTTTAATCATGAAGTAGATACTTTTTCTATTATTGAA
    CAAGCCTTAATGGATCATAAACGTATTTTTGTACCGAAAATGGATTATTTAAATCATCAA
    ATGACTTTTAAAGAGATATTTAATCTCAAAGATATTGATGTCGATAATAAGGGGATTTAC
    TATCCAACTTCAAAAGGTGAAACAACGAATAACCTAGATTTAATTGTTGTTCCTGGTGTT
    GGATTTCAAGACGATGGATATAGAATTGGGTATGGTGGTGGCTATTACGACAGGTTTTTA
    GCTAATTATCAGACAAAGACAATAAGCTTATTATACGATTTTCAAATAACATCATTTGAA
    CCGGAATCATTCGATCAACCAGTCGATAAATTGATTATATATCAAGCAGCATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    594

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1453 [new locus tag: NWMN_RS08200 ]
  • symbol: NWMN_1453
  • description: 5-formyltetrahydrofolate cyclo-ligase
  • length: 197
  • theoretical pI: 5.33118
  • theoretical MW: 22954
  • GRAVY: -0.380711

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.3.2?  ExPASy
    5-formyltetrahydrofolate cyclo-ligase ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate
  • TIGRFAM:
    Metabolism Central intermediary metabolism One-carbon metabolism 5-formyltetrahydrofolate cyclo-ligase (TIGR02727; EC 6.3.3.2; HMM-score: 160.2)
  • TheSEED  :
    • 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2)
    Carbohydrates One-carbon Metabolism One-carbon metabolism by tetrahydropterines  5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2)
    and 2 more
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2)
  • PFAM:
    NADP_Rossmann (CL0063) 5-FTHF_cyc-lig; 5-formyltetrahydrofolate cyclo-ligase family (PF01812; HMM-score: 133.7)
    and 2 more
    no clan defined CTE_SPB4; ATP-dependent rRNA helicase SPB4-like, C-terminal extension (PF13959; HMM-score: 16.1)
    Rep (CL0169) PV_NSP1; Parvovirus non-structural protein 1 (PF12433; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9964
    • Cytoplasmic Membrane Score: 0.0024
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.001
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005353
    • TAT(Tat/SPI): 0.000172
    • LIPO(Sec/SPII): 0.000635
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTCIVINDIIELNKVGDNVTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANAIALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIYYPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITSFEPESFDQPVDKLIIYQAA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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