PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1305 [new locus tag: NWMN_RS07350 ]
• pan locus tag^?: SAUPAN003807000
• symbol: asd
• pan gene symbol^?: asd
• synonym:
• product: aspartate semialdehyde dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1305 [new locus tag: NWMN_RS07350 ]
• symbol: asd
• product: aspartate semialdehyde dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 1438052..1439041
• length: 990
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332108 NCBI
• RefSeq: YP_001332339 NCBI
• BioCyc:
• MicrobesOnline: 3706857 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACAAAGTTAGCAGTTGTGGGTGCAACAGGATTAGTAGGTACAAAAATGTTGGAGACA
  TTAAATCGTAAAAATATTCCTTTCGATGAATTAGTATTATTTTCATCAGCACGTTCTGCA
  GGGCAAGAAGTTGAATTTCAAGGAAAAACATATACAGTTCAAGAATTAACTGATGCTCGT
  GCAAGTGAACATTTCGATTATGTATTAATGAGTGCTGGTGGCGGTACAAGCGAACACTTT
  GCCCCACTTTTTGAAAAAGCTGGTGCAATCGTTATAGACAATTCAAGTCAATGGCGTATG
  GCAGAAGATATTGATTTAATCGTTCCGGAAGTCAATGAACCTACATTTACAAGAGGTATC
  ATTGCCAATCCAAACTGCTCTACGATTCAATCTGTTGTACCTCTAAAAGTATTGCAAGAT
  GCTTATGGTTTAAAACGAGTGGCATATACAACATATCAAGCTGTATCAGGTTCAGGGATG
  AAAGGTAAGAAAGATTTAGCTGAAGGTGTAAATGGTAAAGCACCAGAAGCATATCCACAT
  CCAATTTATAATAATGTGTTACCGCATATTGATGTGTTTTTAGAAAACGGATATACAAAA
  GAAGAACAAAAAATGATTGATGAGACGAGAAAAATTTTAAATGCGCCAGACTTAAAAGTA
  ACAGCAACATGCGCACGTGTGCCTGTTCAAGATAGTCATAGTGTTGAAATTGATGTAACG
  CTTGACAAAGAAACAACAGCAGAAGATATTAAAGCGTTATTTGATCAAGATGACCGCGTT
  GTTTTAGTAGACAATCCAGAGAACAATGAATATCCAATGGCAATCAATTCTACTAATAAA
  GATGAAGTGTTTGTTGGCCGTATACGTAGAGATGATTCATTAGAAAATACTTTCCATGTA
  TGGTGTACATCAGACAATTTATTAAAAGGTGCTGCATTAAATGCTGTACAAGTATTGGAA
  CAAGTTATGCGTTTAAAAGGAGCGAATTAA
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⊟Protein

⊟General

• locus tag: NWMN_1305 [new locus tag: NWMN_RS07350 ]
• symbol: Asd
• description: aspartate semialdehyde dehydrogenase
• length: 329
• theoretical pI: 4.64802
• theoretical MW: 36282.7
• GRAVY: -0.293009

⊟Function

• reaction:
  EC 1.2.1.11?  ExPASy
  Aspartate-semialdehyde dehydrogenase L-aspartate 4-semialdehyde + phosphate + NADP⁺ = L-4-aspartyl phosphate + NADPH
• TIGRFAM:
  Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01296; EC 1.2.1.11; HMM-score: 379.1)
  and 9 more
  Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR00978; EC 1.2.1.11; HMM-score: 126.2)
  Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01745; EC 1.2.1.11; HMM-score: 83.2)
  Metabolism Amino acid biosynthesis Glutamate family N-acetyl-gamma-glutamyl-phosphate reductase (TIGR01850; EC 1.2.1.38; HMM-score: 58.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine erythrose-4-phosphate dehydrogenase (TIGR01532; EC 1.2.1.72; HMM-score: 30)
  Metabolism Energy metabolism Glycolysis/gluconeogenesis glyceraldehyde-3-phosphate dehydrogenase, type I (TIGR01534; EC 1.2.1.-; HMM-score: 27.6)
  Metabolism Amino acid biosynthesis Glutamate family N-acetyl-gamma-glutamyl-phosphate reductase (TIGR01851; EC 1.2.1.38; HMM-score: 27.6)
  glyceraldehyde-3-phosphate dehydrogenase, type II (TIGR01546; EC 1.2.1.59; HMM-score: 16.4)
  Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 14.3)
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions integrating conjugative element protein, PFL_4703 family (TIGR03746; HMM-score: 13.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  GADPH_aa-bio_dh (CL0139) Semialdhyde_dhC; Semialdehyde dehydrogenase, dimerisation domain (PF02774; HMM-score: 154.4)
  and 6 more
  NADP_Rossmann (CL0063) Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 100)
  GADPH_aa-bio_dh (CL0139) Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain (PF02800; HMM-score: 30.2)
  NADP_Rossmann (CL0063) Ldh_1_N; lactate/malate dehydrogenase, NAD binding domain (PF00056; HMM-score: 16.1)
  Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 14.7)
  no clan defined TGT_C1; C1 domain of tRNA-guanine transglycosylase dimerisation (PF14809; HMM-score: 13.2)
  DUF2895; Protein of unknown function (DUF2895) (PF11444; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.033175
  • TAT(Tat/SPI): 0.000893
  • LIPO(Sec/SPII): 0.004506
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221517 NCBI
• RefSeq: YP_001332339 NCBI
• UniProt: A0A0H3KGC8 UniProt

⊟Protein sequence

• MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEVEFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAEDIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGMKGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDLKVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAINSTNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: lysC > asd > dapA > dapB > dapD

⊟Regulation

• regulators: L-box (transcription termination) regulon, CodY (repression) regulon

  L-box	(RNA)	important in Lysine biosynthesis; transcription unit transferred from N315 data RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications