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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1249 [new locus tag: NWMN_RS07050 ]
  • pan locus tag?: SAUPAN003710000
  • symbol: NWMN_1249
  • pan gene symbol?: guaC
  • synonym:
  • product: guanosine 5'-monophosphate oxidoreductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1249 [new locus tag: NWMN_RS07050 ]
  • symbol: NWMN_1249
  • product: guanosine 5'-monophosphate oxidoreductase
  • replicon: chromosome
  • strand: +
  • coordinates: 1372619..1373596
  • length: 978
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGAAAATATTTGATTACGAAGATATTCAATTAATACCTAATAAATGCATAGTTGAAAGT
    AGGTCTGAATGTGATACAACTATCCAATTTGGTCCAAAAAAATTCAAGCTACCTGTAGTT
    CCTGCAAATATGCAAACAGTTATGAATGAGAAATTAGCGAAATGGTTTGCTGAAAATGAT
    TACTTTTATATCATGCATCGTTTTGATGAAGAAGCAAGAATACCTTTTATAAAACATATG
    CAAAATTCAGGCTTATTTGCATCTATTTCAGTTGGTGTAAAGAAAGCGGAATTTGATTTT
    ATTGAAAAGTTAGCTCAAGAAAAATTAATCCCCGAATATATTACAATAGATATTGCGCAT
    GGTCACTCAGATTCAGTGATAAACATGATTAAACATATAAAAACCCATATACCTGATAGT
    TTTGTTATTGCTGGTAATGTTGGTACGCCAGAAGGTGTTAGAGAATTAGAAAATGCTGGT
    GCTGATGCTACCAAAGTCGGTATAGGTCCTGGTAGAGTTTGTATTACAAAGATTAAAACA
    GGTTTTGGTACTGGTGGTTGGCAGTTAGCGGCATTAAACATATGTAGTAAAGCAGCTCGT
    AAACCTTTGATTGCCGATGGTGGTATAAGAACGCATGGCGACATTGCTAAATCAATTAGA
    TTTGGTGCATCAATGGTCATGATTGGTTCATTATTTGCGGCACACGAAGAATCACCTGGT
    GAAACTGTAGAACTTGATGGTAAACAGTATAAAGAATATTTTGGTAGTGCATCTGAATTT
    CAAAAAGGCGAACATAAAAATGTAGAAGGTAAAAAAATGTTTGTAGAACATAAGGGTTCA
    TTAATGGATACCTTAAAAGAAATGCAACAAGATTTACAAAGCTCAATTTCATATGCCGGT
    GGAAAAGACTTGAAATCATTACGTACTGTAGATTATGTTATTGTTAGAAACTCTATTTTC
    AACGGTGATAGAGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    978

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1249 [new locus tag: NWMN_RS07050 ]
  • symbol: NWMN_1249
  • description: guanosine 5'-monophosphate oxidoreductase
  • length: 325
  • theoretical pI: 7.06414
  • theoretical MW: 36116.2
  • GRAVY: -0.279692

⊟Function[edit | edit source]

  • reaction:
    EC 1.7.1.7?  ExPASy
    GMP reductase Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanosine monophosphate reductase (TIGR01306; EC 1.7.1.7; HMM-score: 701)
    and 13 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 186)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanosine monophosphate reductase (TIGR01305; EC 1.7.1.7; HMM-score: 175.2)
    Unknown function General IMP dehydrogenase family protein (TIGR01303; HMM-score: 139.4)
    Unknown function General IMP dehydrogenase family protein (TIGR01304; HMM-score: 58.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 31.5)
    glycosyl amidation-associated protein WbuZ (TIGR03572; HMM-score: 25.2)
    Metabolism Amino acid biosynthesis Histidine family 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (TIGR00007; EC 5.3.1.16; HMM-score: 20.7)
    Metabolism Amino acid biosynthesis Histidine family imidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 17.8)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 15.7)
    heme/flavin dehydrogenase, mycofactocin system (TIGR03966; EC 1.-.-.-; HMM-score: 14.3)
    putative enoyl-[acyl-carrier-protein] reductase II (TIGR03151; EC 1.3.1.-; HMM-score: 13.8)
    Metabolism Amino acid biosynthesis Glutamate family 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase (TIGR01182; EC 4.1.2.14,4.1.3.16; HMM-score: 13.7)
    Metabolism Energy metabolism Entner-Doudoroff 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase (TIGR01182; EC 4.1.2.14,4.1.3.16; HMM-score: 13.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 227.9)
    and 10 more
    His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 26.8)
    FMN_dh; FMN-dependent dehydrogenase (PF01070; HMM-score: 26.3)
    NMO; Nitronate monooxygenase (PF03060; HMM-score: 26.2)
    ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 17.7)
    DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 16.9)
    TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 15.6)
    Glu_synthase; Conserved region in glutamate synthase (PF01645; HMM-score: 13.4)
    Aldolase; KDPG and KHG aldolase (PF01081; HMM-score: 12.8)
    G3P_antiterm; Glycerol-3-phosphate responsive antiterminator (PF04309; HMM-score: 12.4)
    IGPS; Indole-3-glycerol phosphate synthase (PF00218; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007596
    • TAT(Tat/SPI): 0.000195
    • LIPO(Sec/SPII): 0.003336
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANMQTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIEKLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAGADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKSIRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVEHKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_0877(ppnK)inorganic polyphosphate/ATP-NAD kinase  [1] (data from MRSA252)
    NWMN_1476hypothetical protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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