PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0994 [new locus tag: NWMN_RS05580 ]
• pan locus tag^?: SAUPAN001421000
• symbol: NWMN_0994
• pan gene symbol^?: —
• synonym:
• product: phage cI-like repressor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0994 [new locus tag: NWMN_RS05580 ]
• symbol: NWMN_0994
• product: phage cI-like repressor
• replicon: chromosome
• strand: -
• coordinates: 1101519..1102238
• length: 720
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330598 NCBI
• RefSeq: YP_001332028 NCBI
• BioCyc:
• MicrobesOnline: 3706545 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGATAAAAAAGAATTAGCGAAATTTATAGGCAATAAAATCAGATACTATAGAACCAAA
  TTGAACTTAACTCAAGATCAACTTGGAGAAAAACTCAACACTAAAAAGGCTACTATTTCA
  AATTATGAGACAGGGTACAGAACTCCTAAACAAGATGATTTGTTTGAAATTGCTCATATT
  TTAAATATCAGTATCGATGATTTGTTTCCTACAAGAAATAATAAAAAAAACGACATCACT
  TCCATATACAGTAAACTCACGCCTCCAAGACAAAAAAACGTACTTAACTACGCAAATGAG
  CAATTAGATGAACAGAATAAAGTCACTTCTATAGATGAATATAAAGAGTCTAAACTAGTA
  TCGTATATTGCATGTGGTGCAACTGGTGCTGGCATAGGAGAAGAATTATATGATGACATA
  TTGCATGAAGAAGTATTTTTTAAAGAAGACGAAACGCCATCAAATGCTGATTTTTGTATT
  TTAGTTAATGGTGATTCAATGGAACCTATGTTAAAACAAGGAACATACGCTTTTATTAAG
  AAAGAAGATTCTATTAAAGATGGTACAATTGCACTCGTTGTATTAGATGGAGTAAGTCTT
  ATCAAGCGTGTAGATATATGCGAAGACTATATTAATTTGGTATCTCTAAATCCGAAGTAT
  GATGATATCAAAGTCGCTTCGTTTAGTAATATTAAAGTAATGGGCAAAGTTGTATTGTGA
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⊟Protein

⊟General

• locus tag: NWMN_0994 [new locus tag: NWMN_RS05580 ]
• symbol: NWMN_0994
• description: phage cI-like repressor
• length: 239
• theoretical pI: 4.92692
• theoretical MW: 27127.7
• GRAVY: -0.442259

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, y4mF family (TIGR03070; HMM-score: 32.1)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 29)
  Signal transduction Regulatory functions DNA interactions repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 29)
  putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 28.3)
  and 6 more
  Hypothetical proteins Conserved TIGR00270 family protein (TIGR00270; HMM-score: 21.7)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glucose-1-phosphate adenylyltransferase, GlgD subunit (TIGR02092; EC 2.7.7.27; HMM-score: 19.8)
  signal peptidase I (TIGR02228; EC 3.4.21.89; HMM-score: 15.9)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking signal peptidase I (TIGR02227; EC 3.4.21.89; HMM-score: 13.5)
  Cellular processes Cellular processes Detoxification nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 13.2)
  Genetic information processing Protein fate Protein modification and repair nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 13.2)
• TheSEED: data available for COL, NCTC8325
• PFAM:
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 59.2)
  and 7 more
  Peptidase_SF (CL0299) Peptidase_S24; Peptidase S24-like (PF00717; HMM-score: 41.5)
  HTH (CL0123) HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 40.7)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 31.3)
  Pou; Pou domain - N-terminal to homeobox domain (PF00157; HMM-score: 16.1)
  HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 14.7)
  no clan defined BNIP2; Bcl2-/adenovirus E1B nineteen kDa-interacting protein 2 (PF12496; HMM-score: 13.4)
  HTH (CL0123) MqsA_antitoxin; Antitoxin component of bacterial toxin-antitoxin system, MqsA (PF15731; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005387
  • TAT(Tat/SPI): 0.000305
  • LIPO(Sec/SPII): 0.0006
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221206 NCBI
• RefSeq: YP_001332028 NCBI
• UniProt: A0A0H3K845 UniProt

⊟Protein sequence

• MDKKELAKFIGNKIRYYRTKLNLTQDQLGEKLNTKKATISNYETGYRTPKQDDLFEIAHILNISIDDLFPTRNNKKNDITSIYSKLTPPRQKNVLNYANEQLDEQNKVTSIDEYKESKLVSYIACGATGAGIGEELYDDILHEEVFFKEDETPSNADFCILVNGDSMEPMLKQGTYAFIKKEDSIKDGTIALVVLDGVSLIKRVDICEDYINLVSLNPKYDDIKVASFSNIKVMGKVVL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0993 < NWMN_0994

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications