PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0894 [new locus tag: NWMN_RS05000 ]
• pan locus tag^?: SAUPAN003206000
• symbol: NWMN_0894
• pan gene symbol^?: —
• synonym:
• product: 5' nucleotidase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0894 [new locus tag: NWMN_RS05000 ]
• symbol: NWMN_0894
• product: 5' nucleotidase family protein
• replicon: chromosome
• strand: +
• coordinates: 993713..995227
• length: 1515
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330534 NCBI
• RefSeq: YP_001331928 NCBI
• BioCyc:
• MicrobesOnline: 3706443 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGATGGAAAAAAATGAAAACATTAATGTAGAGATTTTAACTACGTCAGATATGCATAGT
  CATTTCTTAAATGGTGATTATGGTTCAAATATTTATAGAGCTGGTACTTATGTTAACCAA
  GTAAGAGCACAAAATCATCGCGTCATTTTATTAGATAGTGGCGGAAGTTTAGCTGGCTCG
  TTAGCGGCCTATTATTATGCTATTGTTGCACCTTATAAACGACATCCAATGATAAAGTTA
  ATGAACAGAATGCATTATGATGCTAGCGGTGTGAGTCCAAGTGAATTCAAGTTTGGTTTA
  TCATTTTTAACTCGTTCAATTGCTTTGGCACGTTTTCCATGGTTATCAGCAAATATTGAA
  TACAATGTTACTAAGGAGCCTTATTTTTCAACTCCATATTGTATTAAACATTTTGGTGAC
  TTAAAAATTGCTATCGTAGGCGTCACAGCAGATGGTTTAATGGAAAATGAGTATTCTGAA
  ATGGAGCAAGATGTATCTATTGAAAAGACATTAGTGGCATCAAAACGTTGGATTAGATAT
  ATCCATGAAGTTGAAGAGCCAGATTTTTTGATTGTAATTTATCATGGTGGATTGAATAAA
  ATTAGTAATAGTACGAAAAATAAAAAGGCAAGTTCGAATGAAGCTGAAAAATTAATGGAA
  GAACTCGGTGTTATAGATTTAATGATTACAGCTCATCAGCATCAAACAATAGTAGGTCAA
  GATCATGAAACGTATTATGTTCAGGCTGGTCAAGATGCCAAAGAGCTTGTACATCTTTCG
  ATTAATTTTAAAAAGAGAACAACAACTTATGATGTTGAAAGCATTGATTCTAAAGTGATT
  GACTTAAATGAGTATGAAGAGGATCAAGAATTATTAGATTTAACATTCTATGATAGAAAA
  GCAGTGGCTTATTGGTCACAGGAAATCATAAGTGATAAAGGTTTGATGTTATCAGTAAAT
  GGGTTACAAGATTTAGTCTGTCAAACACATCCATTTTCGCAATTATTACATGATGCAATT
  CACCTTGCATTTGATAATGATATAACATGTGTCCACGTGCCTATGAACGGAGAGAAGGGG
  TTGAGTGGACAGATTCGAAATGAAGATTTGTATCATGCATACCCATATCCAGATAAGCCA
  ATGGATATGACAATTAGTGGTCAAAATATCAAAGATATATTGGAGTATAGTTATTCACAT
  TTAGATTTTGTTAACGAGCAATTAAGCTTAACAATTATTGATGAAACGTTATGTACAATG
  TGGCAAGGATTCAATTATGAGATTGATATGAATCAAGAACCTGGGCAACGAGTAATGTTA
  GATCAAATTGATTTGACTAAGAGTTATAGAGTTACAATGACTGACTATTGTTATCGTAAC
  TACAAGAATTATTTAAAAAATGCTATTATACATGAATCATACGATGAAACAATGAGTACA
  TTAATTGCAGAGAAGTTAAGAGATCCGAATTATCGTATTTCATGTAGTGATAATTTTATA
  GTTAAAAACAGGTAA
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⊟Protein

⊟General

• locus tag: NWMN_0894 [new locus tag: NWMN_RS05000 ]
• symbol: NWMN_0894
• description: 5' nucleotidase family protein
• length: 504
• theoretical pI: 5.00359
• theoretical MW: 58058.2
• GRAVY: -0.397421

⊟Function

• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other 2',3'-cyclic-nucleotide 2'-phosphodiesterase (TIGR01390; EC 3.1.4.16; HMM-score: 87.8)
  and 1 more
  Genetic information processing Protein synthesis Translation factors translation elongation factor Ts (TIGR00116; HMM-score: 14.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined 5_nucleotid_C; 5'-nucleotidase, C-terminal domain (PF02872; HMM-score: 55.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.060077
  • TAT(Tat/SPI): 0.002964
  • LIPO(Sec/SPII): 0.004422
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151221106 NCBI
• RefSeq: YP_001331928 NCBI
• UniProt: A0A0H3K7Y8 UniProt

⊟Protein sequence

• MMEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQNHRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFLTRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEMEQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLMEELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDSKVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQLLHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDILEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFIVKNR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications