PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0136 [new locus tag: NWMN_RS00750 ]
• pan locus tag^?: SAUPAN001035000
• symbol: NWMN_0136
• pan gene symbol^?: murP
• synonym:
• product: sucrose-specific PTS transporter IIBC component protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0136 [new locus tag: NWMN_RS00750 ]
• symbol: NWMN_0136
• product: sucrose-specific PTS transporter IIBC component protein
• replicon: chromosome
• strand: +
• coordinates: 171234..172688
• length: 1455
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5329999 NCBI
• RefSeq: YP_001331171 NCBI
• BioCyc:
• MicrobesOnline: 3705667 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACCAAAGAACAACAACTTGCAGAACGAATTATTGCTGCAGTAGGTGGTATGGATAAT
  ATAGATAGTGTCATGAACTGTATGACACGTGTGCGTATTAAAGTATTAGATGAGAATAAA
  GTAGATGACCAAGAACTAAGGCATATTGATGGTGTCATGGGTGTTATACACGATGAACGC
  ATTCAAGTTGTGGTTGGACCTGGTACAGTCAATAAAGTGGCTAATCATATGGCGGAATTA
  AGTGGTGTTAAACTAGGTGACCCAATACCACACCATCACAATGATAGTGAAAAAATGGAC
  TATAAATCATATGCAGCTGATAAAGCAAAGGCGAATAAGGAAGCGCATAAAGCAAAACAA
  AAGAATGGTAAGTTGAATAAAGTATTGAAATCAATTGCCAATATCTTTATACCGTTGATT
  CCTGCATTTATTGGAGCTGGATTAATTGGTGGTATTGCAGCAGTACTGAGTAACTTAATG
  GTGGCAGGCTATATTTCAGGTGCTTGGATTACGCAACTTATAACAGTATTTAATGTCATT
  AAAGACGGTATGTTAGCATACTTAGCTATTTTCACTGGTATTAATGCGGCTAAAGAATTT
  GGTGCGACACCAGGACTTGGTGGCGTGATTGGTGGTACAACGTTATTAACGGGTATTGCT
  GGTAAAAATATTTTAATGAATGTCTTCACTGGAGAACCATTGCAACCTGGACAAGGTGGG
  ATTATTGGCGTTATTTTTGCCGTTTGGATTTTAAGTATTGTCGAAAAGAGATTACATAAA
  ATTGTGCCAAATGCGATTGATATTATTGTAACGCCGACTATTGCATTGTTGATTGTAGGA
  CTATTAACTATCTTTATCTTTATGCCATTAGCAGGTTTTGTTTCAGACAGTTTAGTTTCA
  GTAGTTAACGGAATTATTAGTATTGGTGGCGTATTTAGTGGATTTATCATTGGTGCAAGC
  TTCCTACCGTTAGTTATGTTAGGGCTTCATCATATTTTTACGCCAATTCATATAGAAATG
  ATTAACCAATCAGGTGCTACTTACTTATTGCCAATTGCAGCGATGGCTGGTGCTGGACAA
  GTAGGTGCCGCATTAGCACTTTGGGTAAGATGTAAACGCAACACAACATTACGTAATACT
  TTAAAAGGTGCATTGCCAGTTGGTTTCCTAGGTATCGGAGAACCATTAATCTATGGTGTG
  ACTTTGCCATTAGGTCGACCTTTCTTAACTGCTTGTATTGGTGGTGGTATTGGTGGCGCT
  GTAATAGGTGGAATTGGACATATTGGTGCCAAAGCAATAGGCCCAAGTGGTGTGTCACTA
  TTACCATTAATCTCAGATAATATGTATTTAGGTTATATTGCAGGATTACTTGCTGCGTAT
  GCTGGTGGATTCGTTTGTACATATTTATTTGGAACGACAAAGGCGATGCGACAGACAGAT
  TTGTTGGGTGATTAA
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  1455
  

⊟Protein

⊟General

• locus tag: NWMN_0136 [new locus tag: NWMN_RS00750 ]
• symbol: NWMN_0136
• description: sucrose-specific PTS transporter IIBC component protein
• length: 484
• theoretical pI: 9.22825
• theoretical MW: 50664.6
• GRAVY: 0.589463

⊟Function

• TIGRFAM:
  PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 294.4)
  PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 269.8)
  and 10 more
  PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 190.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 119.9)
  Signal transduction Signal transduction PTS PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 119.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 50.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 44.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 43.3)
  Signal transduction Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 43.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 40.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 34.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 30.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 107.8)
  and 1 more
  no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 48.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004499
  • TAT(Tat/SPI): 0.000481
  • LIPO(Sec/SPII): 0.001048
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 151220348 NCBI
• RefSeq: YP_001331171 NCBI
• UniProt: A0A0H3KA42 UniProt

⊟Protein sequence

• MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYKSYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLMVAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTGIAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIALLIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFTPIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGIGEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYLGYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0134 > murQ > NWMN_0136 > NWMN_0137

⊟Regulation

• regulators: MurR* (repression) regulon, CcpA regulon

  MurR*	(TF)	important in N-acetylmuramate utilization; RegPrecise    transcription unit transferred from N315 data RegPrecise
  CcpA	(TF)	important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications