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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_0105 [new locus tag: NWMN_RS00585 ]
- pan locus tag?: SAUPAN000989000
- symbol: capK
- pan gene symbol?: capK
- synonym:
- product: capsular polysaccharide biosynthesis protein CapK
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_0105 [new locus tag: NWMN_RS00585 ]
- symbol: capK
- product: capsular polysaccharide biosynthesis protein CapK
- replicon: chromosome
- strand: +
- coordinates: 129654..130838
- length: 1185
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331804 NCBI
- RefSeq: YP_001331140 NCBI
- BioCyc:
- MicrobesOnline: 3705636 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGGATAGCGTAAAGACAATAATTGGTACGTTGCTTATAGCTTTAGGATTACAATTTTTA
GCTTATCCAATTATTAATCAACGAGTAGGTAATGAAGCGTTCGGTTCTATTTTAACGATT
TATACAATAATAACAATCACGAGTGTTGTATTAGGCAATACGCTTAACAATATACGATTG
ATTAATATGAATCTATACAAATCCAATCATTACTACTGGAAATTTGCATCGATACTTTTA
ATCTCAATTCTGATTGAGAGTATAGCTTTAATTATTGTATTTCTTTACTTTTTTAATTTG
AACATCATCGATATTATCTTTTTAATTCTACTTAATATTTTAATGTGTTTAAGGATTTAT
CTGAATGTATTTTTTAGGATGACTTTAAAATATAATCAGATTTTGTATATTGCTCTTATT
CAATTTTTAGGTTTGCTGATAGGACTATTTCTATATTATTTAACCCAAAACTGGATTGTT
TGTTTTATTACCAGTGAATTGTTTGCAACGATATATACATTGGTTAAATTACGGGGATTA
ACTATAGGCGAGTATCAAAGTGAAGATAATAATGTGGTAAAAGATTATGTGATGCTACTG
AGTACAAATAGCCTTAATAATTTGAATCTCTATTTAGATAGATTAATCTTATTACCAATT
ATAGGTGGAACAGCTGTAACTATATCATTTCTTTCAACATTTATTGGGAAAATGTTAGCT
ACATTTCTATATCCGATTAATAATGTAGTACTTTCATATATTTCTGTAAATGAAAGTGAC
AATATAAAGAAGCAATATTTGAAAACTAATCTAATTGCTATAGCTGCCCTATGTTTAGTC
ATGATTATATGTTATCCAATTACAATAATTATTGTCTCTTTACTGTATAACATTGATTCA
AGTTTATATTCGAAGTTTATTATTTTAGGTAATATAGGTGTTTTATTCAATGCAGTGAGT
ATTATGATCCAAACTTTAAATACAAAACACGCATCAATAACATTACAAGCGAATTATATG
ACGCTTCACACGATTACATTTATATTCATAACTATTTTAATGACAATTGCGTTTGGTCTA
AATGGATTCTTTTGGACAACGCTGTTCAGCAACATTATTAAGTATGTGATTTTAAATATT
ATAGGTTTAAAGTCTAAATTCATTAATAAAAAGGACGTCGATTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_0105 [new locus tag: NWMN_RS00585 ]
- symbol: CapK
- description: capsular polysaccharide biosynthesis protein CapK
- length: 394
- theoretical pI: 9.30526
- theoretical MW: 44854.6
- GRAVY: 0.957107
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 12
- DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.9964
- Cell wall & surface Score: 0
- Extracellular Score: 0.0036
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.233077
- TAT(Tat/SPI): 0.004354
- LIPO(Sec/SPII): 0.013219
- predicted transmembrane helices (TMHMM): 10
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSILTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFLYFFNLNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYYLTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLYLDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKTNLIAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLNTKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKSKFINKKDVD
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulators: CodY (repression) regulon, SigB (activation) regulon
CodY (TF) important in Amino acid metabolism; RegPrecise transcription unit transferred from N315 data RegPrecise SigB (sigma factor) controlling a large regulon involved in stress/starvation response and adaptation; [3] other strains
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Daniela Keinhörster, Shilpa Elizabeth George, Christopher Weidenmaier, Christiane Wolz
Function and regulation of Staphylococcus aureus wall teichoic acids and capsular polysaccharides.
Int J Med Microbiol: 2019, 309(6);151333
[PubMed:31362856] [WorldCat.org] [DOI] (I p) - ↑ S Sau, J Sun, C Y Lee
Molecular characterization and transcriptional analysis of type 8 capsule genes in Staphylococcus aureus.
J Bacteriol: 1997, 179(5);1614-21
[PubMed:9045821] [WorldCat.org] [DOI] (P p) - ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
J Bacteriol: 2011, 193(18);4954-62
[PubMed:21725011] [WorldCat.org] [DOI] (I p)