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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0066 [new locus tag: NWMN_RS00370 ]
  • pan locus tag?: SAUPAN000921000
  • symbol: sbnG
  • pan gene symbol?: sbnG
  • synonym:
  • product: 2-dehydro-3-deoxyglucarate aldolase

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0066 [new locus tag: NWMN_RS00370 ]
  • symbol: sbnG
  • product: 2-dehydro-3-deoxyglucarate aldolase
  • replicon: chromosome
  • strand: +
  • coordinates: 88193..88972
  • length: 780
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATGCAACAACTATCTCTGAAACATAGATTAAACAATGGTGATTCAGTTTATGGCATT
    TTTAATTCTATACCGGACCCATTGATGATCGAGGTTATCGCAGCAAGCGGGTATGACTTT
    GTTGTGATTGATACAGAACACGTGGCGATTAATGATGAGACACTAGCGCATTTAATTCGT
    GCAGCTGAAGCAGCGCATATTATACCAATTGTACGTGTCACTGCAGTGATAGATAGAGAT
    ATCATTAAAGTGTTGGATATGGGTGCGAGAGGTATTATTGTGCCACACGTTAAAGATCGT
    GAGACAGTTGAGCATATTGTGAAATTAAGTCGTTATTACCCGCAAGGATTAAGAAGTTTG
    AATGGTGGTCGCATGGCAAGATTTGGACGTACACCATTACTTGATGCAATGGAGATGGCT
    AATGAGCATATTATGGTGATTGCCATGATAGAAGATGTTGAAGGGGTTATGGCCATTGAC
    GATATAGCACAAGTCGAAGGTTTAGACATGATAGTCGAAGGTGCCGCAGATTTATCGCAG
    TCACTTGGCATACCATGGCAAACGCGTGATGATCAAGTAACATCACATGTTCAACATATT
    TTTGAAGTTGTGAATGCACATGGTAAACATTTTTGTGCATTACCACGTGAAGATGAAGAT
    ATTGCAAAATGGCAGGCACAAGGTGTACAAACATTTATTTTAGGTGATGATCGCGGAAAA
    ATATATCGCCATTTAAGTGCATCTCTAGCGACGTCTAAACAGAAAGGGGATGAAGGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780


⊟Protein[edit | edit source]

Protein Data Bank: 4TV5
Protein Data Bank: 4TV6

⊟General[edit | edit source]

  • locus tag: NWMN_0066 [new locus tag: NWMN_RS00370 ]
  • symbol: SbnG
  • description: 2-dehydro-3-deoxyglucarate aldolase
  • length: 259
  • theoretical pI: 5.14904
  • theoretical MW: 28718.7
  • GRAVY: -0.0440154

⊟Function[edit | edit source]

  • TIGRFAM:
    2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (TIGR02311; EC 4.1.2.-; HMM-score: 131.9)
    2-dehydro-3-deoxyglucarate aldolase (TIGR03239; EC 4.1.2.20; HMM-score: 121.5)
  • TheSEED  :
    • Siderophore staphylobactin biosynthesis protein SbnG
    Iron acquisition and metabolism Siderophores Siderophore Staphylobactin  Siderophore staphylobactin biosynthesis protein SbnG
  • PFAM:
    TIM_barrel (CL0036) HpcH_HpaI; HpcH/HpaI aldolase/citrate lyase (PF03328; HMM-score: 106)
    and 1 more
    CheY (CL0304) PDE8A_N; PDE8A-like, N-terminal domain (PF23198; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9982
    • Cytoplasmic Membrane Score: 0.0003
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0015
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00269
    • TAT(Tat/SPI): 0.000168
    • LIPO(Sec/SPII): 0.000302
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDTEHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETVEHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDDIAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDEDIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]


⊟Relevant publications[edit | edit source]

Suzanne E Dale, Amanda Doherty-Kirby, Gilles Lajoie, David E Heinrichs
Role of siderophore biosynthesis in virulence of Staphylococcus aureus: identification and characterization of genes involved in production of a siderophore.
Infect Immun: 2004, 72(1);29-37
[PubMed:14688077] [WorldCat.org] [DOI] (P p)

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