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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002662
  • pan locus tag?: SAUPAN006428000
  • symbol: hisF
  • pan gene symbol?: hisF
  • synonym:
  • product: imidazole glycerol phosphate synthase subunit HisF

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002662
  • symbol: hisF
  • product: imidazole glycerol phosphate synthase subunit HisF
  • replicon: chromosome
  • strand: -
  • coordinates: 2682242..2683000
  • length: 759
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATTAAAAAACGTATCATTCCATGTTTAGATGTCAAAGATGGTCGTGTCGTTAAAGGG
    ATTCAATTTAAAGGATTAAGGGATATAGGGAATCCTGTTGATTTAGCAATGTATTACAAT
    GAAGCGGGTGCTGATGAGTTAGTATTTTTAGACATCTCTAAGACGGAAGAGGGTCATAGC
    TTAATGCTAGAAGTGATTGAACAGACAGCGTCACGCTTGTTTATCCCTCTTACTGTAGGG
    GGTGGGATTCAAAGTCTCGATGATATTACCCAATTGTTAAATCATGGTGCAGATAAAGTA
    TCATTAAATTCAAGTGCTTTAAAAAATCCACAGCTCATTAAACAAGCGAGTGATAAATTC
    GGTAGACAATGCATCTGTATAGCAATTGATAGCTATTATGATCCTGAAAGAAAAGCACAT
    TATTGTTGTACGCATGGTGGTAAAAAGATGACAAATATTAAAGTATATGACTGGGTACAG
    CAAGTAGAACAGTTAGGTGCAGGTGAGCTCCTCGTTACAAGTATGGGACATGATGGTATG
    AAACAAGGCTTTGATATTGAACACCTAGTAAATATTAAGTCTCTTGTAAATATTCCAATC
    ATTGCTTCTGGTGGTGGTGGCAATGCACAACACTTTGTAGAATTATTTGATCAGACGGAT
    GTTTCTGCAGGTTTAGCTGCAAGTATATTACATGATCGAGAAACGACGGTTCAATCTATT
    AAAGAAGTGATACGGCAAGGGGGTATAGCAGTAAGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    759

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002662
  • symbol: HisF
  • description: imidazole glycerol phosphate synthase subunit HisF
  • length: 252
  • theoretical pI: 6.30456
  • theoretical MW: 27562.5
  • GRAVY: -0.0980159

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family imidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 334.4)
    glycosyl amidation-associated protein WbuZ (TIGR03572; HMM-score: 271.7)
    and 10 more
    Metabolism Amino acid biosynthesis Histidine family 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (TIGR00007; EC 5.3.1.16; HMM-score: 119.5)
    bifunctional HisA/TrpF protein (TIGR01919; EC 5.3.1.16,5.3.1.24; HMM-score: 72.6)
    Unknown function General hisA/hisF family protein (TIGR00734; HMM-score: 33.6)
    Metabolism Amino acid biosynthesis Histidine family phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (TIGR02129; EC 5.3.1.16; HMM-score: 32.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 22.8)
    Unknown function General putative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 17)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 16.1)
    dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 14.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA dihydrouridine synthase A (TIGR00742; EC 1.-.-.-; HMM-score: 13.6)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis fructose-1,6-bisphosphate aldolase, class II (TIGR01859; EC 4.1.2.13; HMM-score: 12.8)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 261.1)
    and 6 more
    Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 30.9)
    TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 25.2)
    NMO; Nitronate monooxygenase (PF03060; HMM-score: 21.7)
    G3P_antiterm; Glycerol-3-phosphate responsive antiterminator (PF04309; HMM-score: 19.8)
    DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 13.2)
    ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9611
    • Cytoplasmic Membrane Score: 0.016
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0227
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003209
    • TAT(Tat/SPI): 0.000081
    • LIPO(Sec/SPII): 0.000378
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQQVEQLGAGELLVTSMGHDGMKQGFDIEHLVNIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HisR* (repression) regulon, CodY (repression) regulon
    HisR*(TF)important in Histidine biosynthesis;  regulation predicted or transferred from N315 and NCTC 8325  [1]
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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