PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002636
• pan locus tag^?: SAUPAN006379000
• symbol: sasF
• pan gene symbol^?: sasF
• synonym:
• product: cell-wall-anchored protein SasF

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002636
• symbol: sasF
• product: cell-wall-anchored protein SasF
• replicon: chromosome
• strand: -
• coordinates: 2646844..2648751
• length: 1908
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  
  ATGGCTAAATATCGAGGGAAACCGTTTCAATTATATGTAAAGTTATCGTGTTCGACAATG
  ATGGCGACAAGTATCATTTTAACAAATATCTTGCCGTACGATGCCCAAGCTGCATCTGAA
  AAGGATACTGAAATTTCAAAAGAGATATTATCTAAGCAAGATTTATTAGACAGAGTTGAC
  AAAGCAATTCGTCAAATTGAGCAATTAAAACAGTTATCGGCTTCATCTAAAGCACATTAT
  AAAGCACAACTAAATGAAGCGAAAACAGCATCGCAAATAGATGAAATCATAAAACGAGCT
  AATGAGTTGGATAGCAAAGACAATAAAAGTTCTCACACTGAAATGAACGGTCAAAGTGAT
  ATAGACAGTAAATTAGATCAATTGCTTAAAGATTTAAATGAGGTTTCTTCAAATGTTGAT
  AGGGGTCAACAAAGTGGCGAGGACGATCTTAATGCAATGAAAAATGATATGTCACAAACG
  GCTACAACAAAATATGGAGAAAAAGATGATAAAAATGATGAAGCAATGGTAAATAAGGCG
  TTAGAAGACCTAGACCATTTGAATCAGCAAATACACAAATCGAAAGATGCATTGAAAGAT
  GCATCGAAAGATCCAGCAGTGTCTACAACAGATAGTAATCATGAAGTAGCTAAAACGCCA
  AATAATGATGGTTCTGGACATGTTGTGTTAAATAAATTCCTTTCAAATGAAGAGAATCAA
  AGCCATAGTAATCGACTCACTGATAAATTACAAGGAAGCGATAAAATTAATCATGCTATG
  ATTGAAAAATTAGCTAAAAATAATGCCTCAACGCAACATTACACATATCATAAACTGAAT
  ACGTTACAATCTTTAGATCAACGTATTGCAAATACGCAACTTCCTAAAAATCAAAAATCA
  GACTTAATGAGCGAAGTAAATAAGACGAAAGAGCGTATAAAAAGTCAACGAAATATTATT
  TTGGAAGAACTTGCACGTACTGATGATAAAAAGTATGCTACACAAAGCATTTTAGAAAGT
  ATATTTAATAAAGACGAGGCAGATAAAATTCTAAAAGATATACGTGTTGATGGTAAAACA
  GATCAACAAATTGCAGATCAAATTACTCGTCATATTGATCAACTATCTCTGACAACGAGT
  GATGATTTATTAACGTCATTGATTGATCAATCACAAGATAAGTCGCTATTGATTTCTCAA
  ATCTTACAAACGAAATTAGGAAAAGCTGAAGCAAATAAATTGGCTAAAGATTGGACGAAT
  AAAGGATTATCAAATCGCCAAATCGTTGACCAATTGAAGAAACATTTTGCATCAACTGGC
  GACACGTCTTCAGATGATATATTAAAAGCAATTTTGAATAATGCCAAAGATAAAAAGCAA
  GCAATTGAAACGATTTTAGCAACACGTATAGAAAGACAAAAGGCAAAATTACTGGCAGAT
  TTAATTACTAAAATAGAAACAGATCAAAATAAAATTTTTAATTTAGTTAAATCGGCATTG
  AATGGTAAAGCGGATGATTTATTGAATTTACAAAAGAGACTCAATCAAACGAAAAAAGAT
  ATAGACTATATTTTATCACCAATAGTAAATCGTCCAAGTTTACTAGATCGATTGAATAAA
  AATGGGAAAACAACGGATTTAAATAAGTTAGCAAATTTAATGAATCAAGGATCAAATTTA
  TTAGACAGTATTCCAGATATACCCACACCAAAGCCAGAAAAGACGTTAACACTTGGTAAA
  GGTAATGGATTGTTAAGTGGATTATTAAATGCTGATGGTAATGTATCTTTGCCTAAAGCG
  GGGGAAACGATAAAAGAACATTGGTTGCCGATATCTGTAATTGTTGGTGCAATGGGTGTA
  CTAATGATTTGGTTATCACGACGCAATAAGTTGAAAAATAAAGCATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1908
  

⊟Protein

⊟General

• locus tag: JSNZ_002636
• symbol: SasF
• description: cell-wall-anchored protein SasF
• length: 635
• theoretical pI: 9.27283
• theoretical MW: 70922.7
• GRAVY: -0.740787

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  B_GA (CL0598) GA; GA module (PF01468; HMM-score: 24.5)
  and 4 more
  no clan defined Poly_export; Polysaccharide biosynthesis/export protein (PF02563; HMM-score: 13.3)
  TRAF (CL0389) Toxin_ToxA; Proteinaceous host-selective toxin ToxA (PF11584; HMM-score: 12.2)
  no clan defined PLN_propep; Protealysin propeptide (PF16485; HMM-score: 10)
  HHH (CL0198) HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 8.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helix: 1
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0.0003
  • Cytoplasmic Membrane Score: 0.019
  • Cell wall & surface Score: 0.4207
  • Extracellular Score: 0.56
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 37 aa
  • SP(Sec/SPI): 0.928204
  • TAT(Tat/SPI): 0.015212
  • LIPO(Sec/SPII): 0.0039
  • Cleavage Site: CS pos: 37-38. AQA-AS. Pr: 0.8836
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTEISKEILSKQDLLDRVDKAIRQIEQLKQLSASSKAHYKAQLNEAKTASQIDEIIKRANELDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQTATTKYGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDALKDASKDPAVSTTDSNHEVAKTPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKNNASTQHYTYHKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYATQSILESIFNKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQDKSLLISQILQTKLGKAEANKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKAILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLLNLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPDIPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIWLSRRNKLKNKA

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications