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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002632
  • pan locus tag?: SAUPAN006373000
  • symbol: manA
  • pan gene symbol?: pmi
  • synonym:
  • product: mannose-6-phosphate isomerase, class I

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002632
  • symbol: manA
  • product: mannose-6-phosphate isomerase, class I
  • replicon: chromosome
  • strand: +
  • coordinates: 2639759..2640697
  • length: 939
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCATTATTTTTACAACCCGTTTTTAAAAACAGAATTTGGGGCGGTCATGCATTAAAA
    GCATTTAACTATGATATCCCTAATGAAACAACTGGTGAATGCTGGGCAATTTCTGCACAT
    CCAAATGGTCCTAATACAATTATCAATGGTCCATACAAGGATATGACCCTAGATCAACTT
    TGGTCACAACATCGTGAATTATTCGATAATGATTCACGAGATAGTTTCCCTCTGCTAACT
    AAAGTATTAGATGCCAATGATAAATTATCTGTTCAAGTTCATCCAGATGATGACTACGCT
    TTAAAACACGAAGGCGAACTAGGTAAAACAGAATGTTGGTATATTTTAGATGCAGCCCCA
    GGTGCTGAAATTATATATGGCGTACATGCACATAATAAACAGACGTTAATCGACATGATT
    GATAATCATGAATTTGACAACCTTTTCAAACGTATACCTGTTAAGCCCGGTGACTTTTAT
    TATGTTCCTGCTGGCACTGTCCATGCCATTGGATCGGGTATTTTAATTTTAGAGACACAA
    CAATCATCCGATACGACATACCGTATTTATGACTATGATAGACGAGATCAAAATGGTCAA
    TTGCGTGATTTGCACTTAGAGCAAAGTAAAGATGTGATTGAATTAGGCAATCATGAACCA
    AATACTACTCCTATAACAACTCACATCGACACACACACTGTAACTCAATTTGTAGATAAT
    CAATATTTTGCAGTATATAAATGGGATATTCATGGAGATTTAAAATTCAATAAACCACAT
    GCTTATTGCTTAGTTACTATTATCGATGGTACTGGTACTTTAACAGTAAACGATAAGCAC
    TATGACATCCAAAAAGGTTCTAGCTTTATTTTAACTACGGAAGACGAGGATATTCACTTT
    GTCGGTAATATTAGTGCTATAGTAAGTCATCCTGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    939

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002632
  • symbol: ManA
  • description: mannose-6-phosphate isomerase, class I
  • length: 312
  • theoretical pI: 5.03622
  • theoretical MW: 35421.2
  • GRAVY: -0.470192

⊟Function[edit | edit source]

  • reaction:
    EC 5.3.1.8?  ExPASy
    Mannose-6-phosphate isomerase D-mannose 6-phosphate = D-fructose 6-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Sugars mannose-6-phosphate isomerase, class I (TIGR00218; EC 5.3.1.8; HMM-score: 226.5)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Cupin (CL0029) PMI_typeI_cat; Phosphomannose isomerase type I, catalytic domain (PF20511; HMM-score: 93.4)
    MPI_cupin_dom; Mannose-6-phosphate isomerase, cupin domain (PF21621; HMM-score: 80.4)
    and 4 more
    Cupin_2; Cupin domain (PF07883; HMM-score: 36)
    AraC_binding; AraC-like ligand binding domain (PF02311; HMM-score: 24.4)
    no clan defined YjbF; Group 4 capsule polysaccharide lipoprotein gfcB, YjbF (PF11102; HMM-score: 12.9)
    Cupin (CL0029) Cupin_3; EutQ-like cupin domain (PF05899; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9976
    • Cytoplasmic Membrane Score: 0.0004
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.002
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.060504
    • TAT(Tat/SPI): 0.002399
    • LIPO(Sec/SPII): 0.008966
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MALFLQPVFKNRIWGGHALKAFNYDIPNETTGECWAISAHPNGPNTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALKHEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDNLFKRIPVKPGDFYYVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNHEPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTVNDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: ManR (activation) regulon, CcpA regulon
    ManR(TF)important in Mannose utilization;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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