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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002621
  • pan locus tag?: SAUPAN006361000
  • symbol: arcC
  • pan gene symbol?: arcC
  • synonym:
  • product: carbamate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002621
  • symbol: arcC
  • product: carbamate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2626486..2627427
  • length: 942
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAGAGAAAATTGTCATTGCATTAGGCGGTAATGCGATACAGACAAAAGAAGCAACA
    GCTGAAGCACAACAAACAGCTATTAGACGTGCGATGCAAAACCTTAAACCTTTATTTGAT
    TCACCAGCGCGTATTGTCATTTCACATGGTAATGGTCCACAAATTGGAAGTTTATTAATC
    CAACAAGCTAAATCGAACAGTGACACAACGCCGGCAATGCCATTGGATACTTGTGGTGCA
    ATGTCACAGGGTATGATAGGCTATTGGTTGGAAACTGAAATCAATCGCATTTTAACTGAA
    ATGAATAGTGATAGAACTGTAGGCACAATCGTTACACGTGTGGAAGTAGATAAAGATGAT
    CCACGATTTGATAACCTAACTAAACCAATTGGTCCTTTTTATACGAAAGAAGAAGTTGAA
    GAATTACAAAAAGAACAGCCAGACTCAGTCTTTAAAGAAGATGCAGGACGTGGTTATAGA
    AAAGTAGTTGCGTCACCACTACCTCAATCTATACTAGAACACCAGTTAATTCGAACTTTA
    GCAGACGGTAAAAATATTGTCATTGCATGCGGTGGTGGCGGTATTCCAGTTATAAAAAAA
    GAAAATACCTATGAAGGTGTTGAAGCGGTTATAGATAAAGATTTTGCTAGTGAGAAATTA
    GCAACGCTGATTGAAGCAGATACCTTAATGATTCTTACGAATGTAGAAAATGTATTTATT
    AACTTTAATGAACCTAATCAACAACAAATCGATGATATTGATGTAGCAACACTGAAAAAA
    TACGCGGCACAAGGTAAGTTTGCGGAAGGATCGATGTTGCCAAAAATAGAAGCTGCGATA
    CGATTTGTTGAAAGTGGGGAAAACAAAAAAGTTATCATTACCAATTTAGAGCAGGCATAC
    GAAGCTTTGATTGGTAATAAAGGTACACACATTCACATGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    942

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002621
  • symbol: ArcC
  • description: carbamate kinase
  • length: 313
  • theoretical pI: 4.82972
  • theoretical MW: 34397
  • GRAVY: -0.292652

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.2.2?  ExPASy
    Carbamate kinase ATP + NH3 + CO2 = ADP + carbamoyl phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines carbamate kinase (TIGR00746; EC 2.7.2.2; HMM-score: 390)
    and 4 more
    Metabolism Amino acid biosynthesis Glutamate family acetylglutamate kinase (TIGR00761; EC 2.7.2.8; HMM-score: 49.2)
    Metabolism Amino acid biosynthesis Glutamate family glutamate 5-kinase (TIGR01027; EC 2.7.2.11; HMM-score: 27)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 19.7)
    Metabolism Amino acid biosynthesis Glutamate family delta l-pyrroline-5-carboxylate synthetase (TIGR01092; HMM-score: 12.9)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 89.8)
    and 1 more
    P-loop_NTPase (CL0023) SKI; Shikimate kinase (PF01202; HMM-score: 20.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9904
    • Cytoplasmic Membrane Score: 0.0019
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0075
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.048602
    • TAT(Tat/SPI): 0.007108
    • LIPO(Sec/SPII): 0.004128
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPARIVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMNSDRTVGTIVTRVEVDKDDPRFDNLTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYRKVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASEKLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKIEAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, ArcR* (activation) regulon, ArgR/AhrC (repression) regulon, CcpA regulon
    Rex*(TF)important in Energy metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    ArcR*(TF)important in Arginine degradation;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    ArgR/AhrC(TF)important in Arginine biosynthesis, Arginine degradation;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 2.2 2.3 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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