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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002564
  • pan locus tag?: SAUPAN006262000
  • symbol: JSNZ_002564
  • pan gene symbol?: —
  • synonym:
  • product: NAD(P)/FAD-dependent oxidoreductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002564
  • symbol: JSNZ_002564
  • product: NAD(P)/FAD-dependent oxidoreductase
  • replicon: chromosome
  • strand: -
  • coordinates: 2571982..2573088
  • length: 1107
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCAACATCATAAAGTGGCTATTATCGGTGCCGGTGCTGCAGGTATAGGTATGGCCATT
    ACCTTAAAAGATTTCGGTATAACAGATGTCATTATTTTAGAAAAAGGAACAGTAGGACAT
    TCATTTAAACATTGGCCGAAATCGACCCTTACGATTACGCCATCATTTACGTCTAATGGA
    TTTGGCATGCCTGATATGAATGCAATTTCCATGGATACTTCACCAGCATTTACATTTAAT
    GAAGAACATATTTCCGGAGAAACATATGCTGAATATTTACAAGTGGTTGCCAACCATTAC
    GAGCTGAATATCTTTGAAAATACAGTTGTCACAAATATATCTGTAGATGATGCATTTTAT
    ACGATTGCAACGACAACAGAGACATATCACGCGGATTATATCTTTGTCGCAACAGGTGAT
    TATAATTTCCCTAAAAAGCCATTTAAATATGGTATTCATTATAGTGAAATTGAAGACTTT
    GATAACTTTAATAAGGGACAATATGTGGTTATCGGAGGTAATGAAAGTGGCTTTGATGCT
    GCATATCAACTTGCAAAAAATGGCTCTGACATCGCACTTTATACTAGCACAACCGGTTTA
    AATGATCCGGATGCTGATCCTAGTGTTAGATTGTCACCTTATACACGTCAGAGACTAGGT
    AACGTCATTAAGCAAGGTGCTCGCATTGAAATGAATGTACATTATACAGTTAAAGATATT
    GATTTTAACAATGGACAGTATCATATCAGTTTTGATAGCGGACAAAGTGTGCATACGCCT
    CATGAACCAATACTAGCAACTGGCTTTGATGCAACAAAAAATCCAATCGTTCAACAATTA
    TTTGTGACAACAAATCAAGATATTAAATTAACAACACATGATGAATCGACACGTTATCCG
    AATATTTTTATGATTGGTGCAACAGTTGAAAATGATAATGCCAAATTATGCTATATCTAT
    AAATTCAGAGCGCGATTTGCAGTACTTGCACATCTTTTAACACAGCGGGAAGGCTTACCA
    GCTAAACAAGAGGTCATTGAAAATTACCAAAAAAATCAAATGTATTTAGATGATTATTCA
    TGTTGTGAAGTGTCATGCACATGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1107

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002564
  • symbol: JSNZ_002564
  • description: NAD(P)/FAD-dependent oxidoreductase
  • length: 368
  • theoretical pI: 5.05655
  • theoretical MW: 41135.7
  • GRAVY: -0.294022

⊟Function[edit | edit source]

  • reaction:
    EC 1.-.-.-?  ExPASy
  • TIGRFAM:
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 96.6)
    and 34 more
    flavin-dependent oxidoreductase, MSMEG_0569 family (TIGR04046; HMM-score: 54.2)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 35.8)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 35.7)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 25.6)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 24.3)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 24.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 20.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 19.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides L-aspartate oxidase (TIGR00551; EC 1.4.3.16; HMM-score: 19.3)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 19.3)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 18.3)
    Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 18.3)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 17.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 16.8)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, monomeric form (TIGR01377; HMM-score: 16.6)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, beta subunit family (TIGR01373; HMM-score: 16.3)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 16.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 16)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 15.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 15.3)
    Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 14.5)
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 14.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 14.3)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 14.2)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 13.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other phytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 13)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 12.8)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 12.4)
    Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 12.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin protoporphyrinogen oxidase (TIGR00562; EC 1.3.3.4; HMM-score: 11.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 11.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA:m(5)U-54 methyltransferase (TIGR00137; EC 2.1.1.74; HMM-score: 11)
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 11)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 7.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 331.1)
    and 13 more
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 62)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 35)
    HI0933_like; HI0933-like protein Rossmann domain (PF03486; HMM-score: 35)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 30.2)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 25.6)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 22.9)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 22.3)
    Lys_Orn_oxgnase; L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase (PF13434; HMM-score: 20.4)
    Thi4; Thi4 family (PF01946; HMM-score: 19.6)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 19)
    KARI_N; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 17.3)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 14.2)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9533
    • Cytoplasmic Membrane Score: 0.0414
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0052
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.136263
    • TAT(Tat/SPI): 0.00318
    • LIPO(Sec/SPII): 0.033231
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKHWPKSTLTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYELNIFENTVVTNISVDDAFYTIATTTETYHADYIFVATGDYNFPKKPFKYGIHYSEIEDFDNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQRLGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVHTPHEPILATGFDATKNPIVQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLTQREGLPAKQEVIENYQKNQMYLDDYSCCEVSCTC

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Zur* (repression) regulon
    Zur*(TF)important in Zinc homeostasis;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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