PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002525
• pan locus tag^?: SAUPAN006207000
• symbol: JSNZ_002525
• pan gene symbol^?: mvaS
• synonym:
• product: hydroxymethylglutaryl-CoA synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002525
• symbol: JSNZ_002525
• product: hydroxymethylglutaryl-CoA synthase
• replicon: chromosome
• strand: +
• coordinates: 2530668..2531834
• length: 1167
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  
  ATGACAATAGGTATCGATAAAATAAACTTTTACGTTCCAAAGTACTATGTAGACATGGCT
  AAATTAGCAGAAGCACGCCAAGTAGATCCAAACAAATTTTTAATTGGTATTGGTCAAACT
  GAAATGGCTGTTAGTCCTGTAAACCAAGACATCGTTTCAATGGGCGCTAATGCTGCTAAG
  GACATTATAACAGACGAAGACAAAAAGAAAATTGGTATGGTAATTGTGGCAACTGAATCA
  GCAGTTGATGCTGCTAAAGCAGCTGCTGTTCAAATTCACAACTTATTAGGTATTCAACCT
  TTTGCACGCTGCTTTGAAATGAAAGAAGCTTGTTATGCTGCAACACCAGCAATTCAATTA
  GCTAAAGATTATTTAGCAACTAGACCGAATGAAAAAGTATTAGTTATTGCTACAGATACA
  GCACGTTATGGATTGAATTCAGGCGGCGAGCCAACACAAGGTGCTGGCGCAGTTGCGATG
  GTTATTGCACATAATCCAAGCATTTTGGCATTAAATGAAGATGCTGTTGCTTACACTGAA
  GACGTTTATGATTTCTGGCGTCCAACTGGACATAAATATCCATTAGTTGATGGTGCATTA
  TCTAAAGATGCTTATATCCGCTCATTCCAACAAAGCTGGAATGAATACGCAAAACGTCAA
  GGTAAGTCGCTAGCTGACTTCGCATCTCTATGCTTCCATGTTCCATTTACAAAAATGGGT
  AAAAAGGCATTAGAGTCAATCATTGATAACGCTGATGAAACAACTCAAGAGCGTTTACGT
  TCAGGATATGAAGATGCTGTAGATTATAACCGTTATGTCGGTAATATTTATACTGGATCA
  TTATATTTAAGCCTAATATCATTACTTGAAAATCGAGATTTACAAGCTGGTGAAACAATC
  GGTTTATTCAGTTATGGCTCAGGTTCAGTTGGTGAATTTTATAGTGCGACATTAGTTGAA
  GGCTACAAAGATCATTTAGATCAAGCTGCACATAAAGCATTATTAAATAACCGTACTGAA
  GTATCTGTTGATGCATATGAAACATTCTTCAAACGTTTTGATGACGTTGAATTTGACGAA
  GAACAAGATGCTGTTCATGAAGATCGTCATATTTTCTACTTATCAAATATTGAAAATAAC
  GTTCGTGAATATCACAGACCAGAGTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1167
  

⊟Protein

⊟General

• locus tag: JSNZ_002525
• symbol: JSNZ_002525
• description: hydroxymethylglutaryl-CoA synthase
• length: 388
• theoretical pI: 4.67485
• theoretical MW: 43205.2
• GRAVY: -0.295361

⊟Function

• reaction:
  EC 2.3.3.10?  ExPASy
  Hydroxymethylglutaryl-CoA synthase Acetyl-CoA + H₂O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
• TIGRFAM:
  hydroxymethylglutaryl-CoA synthase (TIGR01835; EC 2.3.3.10; HMM-score: 531.4)
  and 3 more
  hydroxymethylglutaryl-CoA synthase (TIGR01833; EC 2.3.3.10; HMM-score: 160.5)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative hydroxymethylglutaryl-CoA synthase (TIGR00748; EC 2.3.3.10; HMM-score: 98.4)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] synthase III (TIGR00747; EC 2.3.1.180; HMM-score: 43.1)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  Thiolase (CL0046) HMG_CoA_synt_C; Hydroxymethylglutaryl-coenzyme A synthase C terminal (PF08540; HMM-score: 96.8)
  and 4 more
  HMG_CoA_synt_N; Hydroxymethylglutaryl-coenzyme A synthase N terminal (PF01154; HMM-score: 68.9)
  Chal_sti_synt_N; Chalcone and stilbene synthases, N-terminal domain (PF00195; HMM-score: 27)
  ACP_syn_III; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III (PF08545; HMM-score: 23.5)
  ACP_syn_III_C; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal (PF08541; HMM-score: 18.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9802
  • Cytoplasmic Membrane Score: 0.0044
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.015
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006327
  • TAT(Tat/SPI): 0.000235
  • LIPO(Sec/SPII): 0.000473
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAVSPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFARCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAMVIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAKRQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNIYTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKALLNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications