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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

organism: Staphylococcus aureus JSNZ
locus tag: JSNZ_002509
pan locus tag^?: SAUPAN006183000
symbol: sdaAA
pan gene symbol^?: sdaAA
synonym:
product: L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: JSNZ_002509
symbol: sdaAA
product: L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha
replicon: chromosome
strand: -
coordinates: 2514602..2515501
length: 900
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID:
RefSeq:
BioCyc:
MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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ATGTTTGATTCAATTAGAGAGACTATAGATTATGCCGTAGAAAATAATATGTCATTTGCG
GATATCATGGTTAAAGAAGAAATGGAATTAAGCGGTAAATCACGTGATGAAGTGCGAGCG
CAAATGAAACAAAATTTAGATGTCATGCGAGACGCAGTAATCAAAGGGACGACAGGTGAT
GGCGTTGAAAGTGTAACGGGCTACACTGGTCATGACGCTGCTAAACTACGTGATTATAAT
GAAACACATCATGCTTTGTCTGGATATGAAATGATTGACGCAGTCAAAGGTGCCATTGCA
ACAAATGAAGTCAATGCTGCGATGGGTATTATTTGTGCAACGCCAACAGCTGGTTCCTCG
GGTACCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTAACAGAAGAGCAA
ATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAATGCAAGT
GTAGCTGGTGCAACAGGTGGCTGCCAAGCTGAAGTTGGTTCAGCATCTGCAATGGCCGCA
GCTGCAGCAGTAGCTATATTCGGAGGATCACCAGAAGCATCCGGGCACGCTATGGCATTA
GCGATAAGTAATTTATTAGGTTTAGTTTGTGACCCAGTAGCCGGACTTGTTGAAATTCCT
TGTGTTATGAGAAATGCAATTGGTTCGGGTAACGCTTTAATTTCAGCAGATTTAGCATTA
GCAGGTATTGAGAGTAGAATCCCTGTTGATGAAGTTATTGAAGCAATGGATAAGGTTGGT
CGTAACCTTCCTGCATCATTACGTGAAACTGGATTGGGTGGACTAGCAGGCACACCAACT
GGCGAAGCAATTAAACGTAAAATCTTTGGCACAGCTGAAGATATGGTTAAAAATAATTAA
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: JSNZ_002509
symbol: SdaAA
description: L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha
length: 299
theoretical pI: 4.42838
theoretical MW: 30971
GRAVY: 0.100334

⊟Function[edit | edit source]

reaction:
EC 4.3.1.17? ExPASy
L-serine ammonia-lyase L-serine = pyruvate + NH₃
TIGRFAM:
Metabolism Energy metabolism Amino acids and amines L-serine dehydratase, iron-sulfur-dependent, alpha subunit (TIGR00718; EC 4.3.1.17; HMM-score: 374.2)
Metabolism Energy metabolism Glycolysis/gluconeogenesis L-serine dehydratase, iron-sulfur-dependent, alpha subunit (TIGR00718; EC 4.3.1.17; HMM-score: 374.2)
and 2 more
Metabolism Energy metabolism Amino acids and amines L-serine ammonia-lyase (TIGR00720; EC 4.3.1.17; HMM-score: 188.9)
Metabolism Energy metabolism Glycolysis/gluconeogenesis L-serine ammonia-lyase (TIGR00720; EC 4.3.1.17; HMM-score: 188.9)
TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
PFAM:
SDH_alpha (CL0722) SDH_alpha; Serine dehydratase alpha chain (PF03313; HMM-score: 262.2)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9396
- Cytoplasmic Membrane Score: 0.0081
- Cell wall & surface Score: 0.0003
- Extracellular Score: 0.052
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.008833
- TAT(Tat/SPI): 0.001752
- LIPO(Sec/SPII): 0.00055
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI:
RefSeq:
UniProt:

⊟Protein sequence[edit | edit source]

MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQNLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATNEVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNASVAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVEIPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLAGTPTGEAIKRKIFGTAEDMVKNN

⊟Experimental data[edit | edit source]

experimentally validated: data available for NCTC8325
protein localization:
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

Operon-mapper ^[1] : JSNZ_002507 < JSNZ_002508 < sdaAA < sdaAB < JSNZ_002511

⊟Regulation[edit | edit source]

regulator: CcpA regulon
CcpA (TF) important in Carbon catabolism; regulation predicted or transferred from N315 and NCTC 8325 ^[2]

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p)
↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
Curr Res Microb Sci: 2025, 9;100489
[PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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