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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

organism: Staphylococcus aureus JSNZ
locus tag: JSNZ_002421
pan locus tag^?: SAUPAN005999000
symbol: JSNZ_002421
pan gene symbol^?: opuCB
synonym:
product: ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: JSNZ_002421
symbol: JSNZ_002421
product: ABC transporter permease
replicon: chromosome
strand: -
coordinates: 2422242..2422877
length: 636
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID:
RefSeq:
BioCyc:
MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
ATGATCGAGTTCCTACATGAACATGGTGGACAGTTGATGTCGAAAACACTGGAACATTTC
TATATTTCTATAGTGGCATTATTACTTGCCATCATTGTTGCAGTACCTATAGGCATTTTA
TTATCAAAAACAAAGCGAACTGCCAATATTATATTAACTGTGGCAGGTGTCTTACAAACT
ATTCCAACACTAGCTGTACTTGCTATTATGATACCGATTTTTGGTGTTGGTAAAACGCCT
GCAATTGTAGCGCTATTTATTTATGTATTATTACCTATTTTAAATAACACGGTGCTCGGT
GTTCAAAATATTGATAGCAACATTAAAGAAGCTGGAAAAAGTATGGGAATGACACAATTT
CAATTGATGAAGGATGTTGAATTGCCGTTAGCATTGCCGCTTATCATTGGTGGCATTCGT
TTGTCATCTGTGTATGTAATTAGTTGGGCTACACTTGCAAGTTATGTAGGTGCGGGTGGA
TTAGGTGATTTCATTTTCAATGGTTTAAATTTATATGATCCACTGATGATTGTAACTGCA
ACGGTACTCGTTACTGTACTAGCATTAGGTGTTGATGCCTTATTAGCTTTAGTTGAAAAA
TGGGTAGTTCCCAAAGGCTTAAAAGTATCTGGATAA
60
120
180
240
300
360
420
480
540
600
636

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: JSNZ_002421
symbol: JSNZ_002421
description: ABC transporter permease
length: 211
theoretical pI: 8.84699
theoretical MW: 22428
GRAVY: 1.05024

⊟Function[edit | edit source]

TIGRFAM:
choline ABC transporter, permease protein (TIGR03416; HMM-score: 96.5)
and 8 more
Metabolism Transport and binding proteins Anions nitrate ABC transporter, permease protein (TIGR01183; HMM-score: 60.8)
Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 50)
Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 24.4)
NifC-like ABC-type porter (TIGR01581; HMM-score: 22.3)
Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 20.9)
Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 20.2)
Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 16.4)
ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 9.8)
TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
PFAM:
BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 88.7)
and 1 more
no clan defined DUF4229; Protein of unknown function (DUF4229) (PF14012; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 5
DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 1
- Cell wall & surface Score: 0
- Extracellular Score: 0
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003909
- TAT(Tat/SPI): 0.000224
- LIPO(Sec/SPII): 0.001513
predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

GI:
RefSeq:
UniProt:

⊟Protein sequence[edit | edit source]

MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKTKRTANIILTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQNIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGGLGDFIFNGLNLYDPLMIVTATVLVTVLALGVDALLALVEKWVVPKGLKVSG

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell:
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

Operon-mapper ^[1] : JSNZ_002419 < JSNZ_002420 < JSNZ_002421 < JSNZ_002422

⊟Regulation[edit | edit source]

regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; regulation predicted or transferred from N315 and NCTC 8325 ^[2]

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p)
↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
Curr Res Microb Sci: 2025, 9;100489
[PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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