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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002394
  • pan locus tag?: SAUPAN005961000
  • symbol: hlgB
  • pan gene symbol?: hlgB
  • synonym:
  • product: bi-component gamma-hemolysin HlgAB/HlgCB subunit B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002394
  • symbol: hlgB
  • product: bi-component gamma-hemolysin HlgAB/HlgCB subunit B
  • replicon: chromosome
  • strand: +
  • coordinates: 2391503..2392480
  • length: 978
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAATGAATAAATTAGTCAAATCATCCGTTGCTACATCTATGGCATTATTATTACTT
    TCTGGTACTGCTAATGCTGAAGGTAAAATAACACCAGTCAGCGTAAAAAAAGTCGATGAC
    AAAGTTACTTTATACAAAACAACAGCCACAGCAGATTCTGATAAGTTTAAAATTTCACAG
    ATTTTAACATTTAATTTCATCAAAGATAAAAGTTATGATAAAGATACTTTAGTACTTAAA
    GCTACTGGGAATATTAACTCAGGCTTTGTGAAACCTAATCCTAATGACTATGACTTTTCA
    AAATTATATTGGGGAGCTAAATACAATGTATCTATAAGCTCACAATCTAATGATTCAGTA
    AACGTCGTTGATTATGCACCAAAAAATCAAAATGAAGAGTTTCAAGTTCAAAATACTTTA
    GGCTATACATTTGGTGGTGACATTAGTATCTCTAATGGTTTATCTGGTGGACTTAATGGA
    AATACAGCTTTTTCTGAAACAATTAATTATAAACAAGAAAGTTACAGAACAACATTAAGT
    CGCAACACAAATTATAAAAATGTTGGCTGGGGAGTTGAAGCACATAAAATTATGAATAAT
    GGTTGGGGACCTTATGGACGAGATAGCTTCCACCCAACATATGGTAATGAACTCTTCTTA
    GCTGGCAGACAAAGCAGTGCATACGCTGGCCAAAACTTCATAGCGCAACACCAAATGCCA
    TTATTATCTAGAAGTAACTTCAATCCAGAATTTTTAAGCGTACTATCACACAGACAAGAT
    GGCGCTAAAAAATCTAAAATTACAGTAACTTATCAACGTGAAATGGATTTATACCAAATT
    CGTTGGAATGGCTTCTACTGGGCAGGCGCAAATTATAAAAACTTTAAAACTAGAACATTT
    AAATCAACATATGAAATTGATTGGGAAAATCACAAAGTGAAATTGTTAGATACAAAAGAA
    ACTGAAAACAATAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    978

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002394
  • symbol: HlgB
  • description: bi-component gamma-hemolysin HlgAB/HlgCB subunit B
  • length: 325
  • theoretical pI: 9.81402
  • theoretical MW: 36710.8
  • GRAVY: -0.669538

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance beta-channel forming cytolysin (TIGR01002; HMM-score: 413.2)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 328.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.9997
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 26 aa
    • SP(Sec/SPI): 0.969612
    • TAT(Tat/SPI): 0.001064
    • LIPO(Sec/SPII): 0.028416
    • Cleavage Site: CS pos: 26-27. ANA-EG. Pr: 0.8456
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTLYKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKLYWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNGNTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNELFLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMDLYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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