From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002188
  • pan locus tag?: SAUPAN005676000
  • symbol: JSNZ_002188
  • pan gene symbol?: rpoA
  • synonym:
  • product: DNA-directed RNA polymerase subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002188
  • symbol: JSNZ_002188
  • product: DNA-directed RNA polymerase subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 2202531..2203475
  • length: 945
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGATAGAAATCGAAAAACCTAGAATTGAGACAATTGAAATTAGTGAAGATGCTAAATTC
    GGTAAGTTCGTTGTTGAACCACTAGAACGTGGCTACGGTACTACACTAGGAAACTCCTTA
    CGTCGTATCCTACTATCTTCATTACCAGGTGCAGCCGTTAAGTATATTGAAATTGAGGGA
    GTTTTACATGAATTCTCAGCAGTAGACAATGTAGTTGAAGATGTTTCTACAATTATTATG
    AACATTAAACAATTAGCATTGAAAATTTACTCTGAAGAAGATAAAACTTTAGAAATTGAT
    GTACGTGATGAAGGCGAAGTAACAGCAAGCGACATTACACATGATAGTGATGTTGAAATT
    TTAAACCCAGAGCTTAAAATTGCAACAGTATCTAAAGGTGGTCACTTAAAAATTCGTCTA
    GTTGCTAACAAGGGTAGAGGTTACGCATTAGCAGAACAAAATAATACTAGTGATTTACCA
    ATTGGTGTAATCCCTGTTGATTCATTGTATTCACCTGTTGAACGTGTGAACTATACTGTT
    GAAAATACACGTGTAGGTCAAAGCAGTGATTTTGATAAATTAACATTGGATGTTTGGACT
    AATGGTTCAATCACACCACAAGAATCAGTTTCATTAGCAGCAAAAATAATGACTGAACAC
    TTGAATATCTTCGTTGGTCTTACTGATGAAGCGCAAAACGCTGAAATCATGATTGAAAAA
    GAAGAAGATCAAAAAGAAAAAGTATTAGAAATGTCTATTGAAGAATTAGACTTATCTGTA
    CGTTCATATAACTGCTTAAAACGCGCAGGAATCAATTCTGTTCAAGAGTTAGCTGACAAA
    TCTGAAGCTGACATGATGAAAGTGCGTAATTTAGGTCGTAAATCTTTAGAAGAAGTTAAA
    TACAAATTAGAAGATTTAGGATTAGGATTAAGAAAAGAAGATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    945

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002188
  • symbol: JSNZ_002188
  • description: DNA-directed RNA polymerase subunit alpha
  • length: 314
  • theoretical pI: 4.39431
  • theoretical MW: 35011.5
  • GRAVY: -0.302229

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.6?  ExPASy
    DNA-directed RNA polymerase Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
  • TIGRFAM:
    Genetic information processing Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase, alpha subunit (TIGR02027; EC 2.7.7.6; HMM-score: 390.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined RNA_pol_A_bac; RNA polymerase Rpb3/RpoA insert domain (PF01000; HMM-score: 120.6)
    HHH (CL0198) RNA_pol_A_CTD; Bacterial RNA polymerase, alpha chain C terminal domain (PF03118; HMM-score: 99.2)
    and 4 more
    RBP11-like (CL0509) RNA_pol_L; RNA polymerase Rpb3/Rpb11 dimerisation domain (PF01193; HMM-score: 92.7)
    OB (CL0021) DUF961; Bacterial protein of unknown function (DUF961) (PF06125; HMM-score: 16.9)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 15.7)
    SAM (CL0003) SAM_EPH; Ephrin receptor 1 SAM domain-like (PF22993; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9352
    • Cytoplasmic Membrane Score: 0.0017
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.063
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007843
    • TAT(Tat/SPI): 0.013642
    • LIPO(Sec/SPII): 0.000555
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVRDEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLPIGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMTEHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQELADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_002188&oldid=107829"