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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002107
  • pan locus tag?: SAUPAN005444000
  • symbol: czrB
  • pan gene symbol?: czrB
  • synonym:
  • product: CDF family zinc efflux transporter CzrB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002107
  • symbol: czrB
  • product: CDF family zinc efflux transporter CzrB
  • replicon: chromosome
  • strand: +
  • coordinates: 2118567..2119544
  • length: 978
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCTCATTCACATCATCATGACCATATGCATAGTCATGTAACTACAAATAATAAGAAA
    GTATTGTTTATATCGTTTTTAATAATCGGTCTATATATGTTTATCGAAATCATCGGCGGT
    CTCCTTGCTAACAGCTTGGCATTACTATCTGACGGTATCCATATGTTTAGCGACACATTC
    TCATTAGGTGTTGCACTTGTCGCATTTATTTATGCTGAAAAGAATGCCACAACTACAAAA
    ACATTTGGTTATAAACGTTTCGAAGTACTCGCAGCGTTATTTAACGGTGTAACGCTTTTT
    GTAATAAGTATTTTGATTGTTTTTGAAGCGATTAAACGTTTCTTTATTCCTTCTGAAGTT
    CAATCAAAAGAAATGTTAATCATTAGTATTATCGGTTTAATTGTCAATATCGTTGTTGCA
    TTCTTTATGTTTAAAGGCGGCGACACTTCACACAATTTAAATATGCGTGGTGCTTTTCTA
    CATGTTATCGGAGACTTATTAGGTTCAGTTGGCGCCATTACTGCAGCTATTTTAATTTGG
    GCATTTGGATGGACAATCGCCGATCCTATCGCAAGTATTTTAGTTTCCGTTATTATTTTA
    AAAAGTGCTTGGGGTATCACAAAATCTTCAATTAACATTTTAATGGAAGGCACACCAAGT
    GATGTTGATATAGATGAAGTTATAACTACTATTAAAAAGGATTCACGAATACAAAGTGTG
    CATGATTGCCATGTTTGGACAATTTCAAATGATATGAATGCATTGAGTTGTCATGTTGTT
    GTAGACCATACATTGACAATGAAAGAATGTGAATTATTATTAGAAAACATTGAGCATGAT
    TTATTACATTTAAATATTCACCATATGACTATTCAATTAGAAACGCCTAATCACAAACAT
    GATGAATCGATTATATGTTCAGGAACACATAGTCATTCACATAACCATCATGCTCATCAT
    CACGCGCATGTACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    978

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002107
  • symbol: CzrB
  • description: CDF family zinc efflux transporter CzrB
  • length: 325
  • theoretical pI: 6.73555
  • theoretical MW: 36142.8
  • GRAVY: 0.426154

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation diffusion facilitator family transporter (TIGR01297; HMM-score: 330)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined Cation_efflux; Cation efflux family (PF01545; HMM-score: 166.9)
    and 2 more
    FtsK_4TM; 4TM region of DNA translocase FtsK/SpoIIIE (PF13491; HMM-score: 19)
    ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 10.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 1
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.027348
    • TAT(Tat/SPI): 0.000197
    • LIPO(Sec/SPII): 0.000988
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSHSHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLANSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFVISILIVFEAIKRFFIPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAFLHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEGTPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLENIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CzrA (repression) regulon
    CzrA(TF)important in Zinc resistance;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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