PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002043
• pan locus tag^?: SAUPAN005355000
• symbol: murF
• pan gene symbol^?: murF
• synonym:
• product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002043
• symbol: murF
• product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
• replicon: chromosome
• strand: -
• coordinates: 2059785..2061143
• length: 1359
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGATTAATGTTACATTAAAGCAAATTCAATCATGGATTCCTTGTGAAATTGAAGATCAA
  TTTTTAAATCAAGAGATAAATGGAGTCACAATTGATTCACGAGCAATTTCTAAAAATATG
  TTATTTATACCATTTAAAGGTGAAAATGTTGACGGTCATCGCTTTGTCTCTAAAGCATTA
  CAAGATGGTGCTGGGGCTGCTTTTTATCAAAGAGGAACACCTATAGATGAAAATGTAAGC
  GGGCCTATTATATGGGTTGAAGACACATTAATGGCATTACAACAATTGGCACAAGCTTAC
  TTGAGACATGTAAACCCTAAAGTAATTGCCGTCACAGGGTCTAATGGTAAAACAACGACT
  AAAGATATGATTGAAAGTGTATTGCATACCGAATTTAAAGTTAAGAAAACGCAAGGTAAT
  TACAATAATGAAATTGGTTTACCTTTAACTATTTTGGAATTAGATAATGATACTGAAATA
  TCAATATTGGAGATGGGGATGTCAGGTTTCCATGAAATTGAATTTCTGTCAAACCTCGCT
  CAACCAGATATTGCAGTTATAACTAATATTGGTGAGTCACATATGCAAGATTTAGGTTCG
  CGCGAGGGGATTGCTAAAGCTAAATCTGAAATTACAATAGGTCTAAAAGATAATGGTACG
  TTTATATATGATGGCGATGAACCATTATTGAAACCACATGTTAAAGAAGTTGAAAATGCA
  AAATGTATTAGTATTGGTGTTGCTACTGATAATGCATTAGTTTGTTCTGTTGATGATAGA
  GATACTACAGGTATTTCATTTACGATTAATAATAAAGAACATTACGATCTGCCAATATTA
  GGAAAGCATAATATGAAAAATGCGACGATTGCCATTGCGGTTGGTCATGAATTAGGTTTG
  ACATATAACACAATCTATCAAAATTTAAAAAATGTCAGCTTAACTGGTATGCGTATGGAG
  CAACATACATTAGAAAATGATATTACTGTGATAAATGATGCCTATAATGCAAGTCCTACA
  AGTATGAGAGCAGCTATTGATACACTGGGTACTTTGACAGGGCGTCGCATTCTAATTTTA
  GGAGATGTTTTAGAATTAGGTGAAAATAGCAAAGAAATGCATATCGGTGTAGGTAATTAT
  TTAGAAGAAAAGCATATAGATGTGTTGTATACGTTTGGTAATGAAGCGAAGTATATTTAT
  GATTCGGGCCAGCAACATGTCGAAAAAGCACAACACTTCAATTCTAAAGACGATATGATA
  GAAGTTTTAACAAGCGATTTAAAAGCGCATGACCGTGTATTAGTTAAAGGATCACGTGGT
  ATGAAGTTAGAAGAAGTTGTAAATGCTTTAATTTCATAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1359
  

⊟Protein

⊟General

• locus tag: JSNZ_002043
• symbol: MurF
• description: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
• length: 452
• theoretical pI: 4.83726
• theoretical MW: 50013.4
• GRAVY: -0.235619

⊟Function

• reaction:
  EC 6.3.2.10?  ExPASy
  UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 452.5)
  and 7 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 110.3)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 93.1)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 79.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 53.3)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 46.9)
  Cell structure Cell envelope Other poly-gamma-glutamate synthase PgsB (TIGR04012; EC 6.3.2.-; HMM-score: 26.9)
  Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 16)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  P-loop_NTPase (CL0023) Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 152.1)
  and 3 more
  Mur_ligase_C_sf (CL0794) Mur_ligase_C; Mur ligase, glutamate ligase domain (PF02875; HMM-score: 84.1)
  MurF-HprK_N (CL0365) Mur_ligase; Mur ligase family, catalytic domain (PF01225; HMM-score: 46.7)
  no clan defined BipA_C; TypA/BipA C-terminal domain (PF21018; HMM-score: 13.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9984
  • Cytoplasmic Membrane Score: 0.0009
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0003
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003635
  • TAT(Tat/SPI): 0.000156
  • LIPO(Sec/SPII): 0.00056
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIPFKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLMALQQLAQAYLRHVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEISILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDNGTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYDLPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDAYNASPTSMRAAIDTLGTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTFGNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNALIS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications