PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002042
• pan locus tag^?: SAUPAN005354000
• symbol: JSNZ_002042
• pan gene symbol^?: cshA
• synonym:
• product: DEAD/DEAH box helicase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002042
• symbol: JSNZ_002042
• product: DEAD/DEAH box helicase
• replicon: chromosome
• strand: -
• coordinates: 2057748..2059268
• length: 1521
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  
  TTGCAAAATTTTAAAGAACTAGGGATTTCGGATAATACGGTTCAGTCACTTGAATCAATG
  GGATTTAAAGAGCCGACACCTATCCAAAAAGACAGTATCCCTTATGCGTTACAAGGAATT
  GATATCCTTGGGCAAGCTCAAACCGGTACAGGTAAAACAGGAGCATTCGGTATTCCTTTA
  ATTGAGAAAGTAGTAGGGAAACAAGGGGTTCAATCGTTGATTTTAGCACCTACAAGAGAA
  TTGGCAATGCAGGTAGCTGAACAATTAAGAGAATTTAGCCGTGGACAAGGTGTCCAAGTT
  GTTACTGTATTCGGTGGTATGCCTATCGAACGCCAAATTAAAGCCTTGAAAAAAGGCCCA
  CAAATCGTAGTCGGAACACCTGGGCGTGTTATCGACCATTTAAATCGTCGCACATTAAAA
  ACTGACGGAATTCATACTTTGATTTTAGATGAAGCTGATGAAATGATGAATATGGGATTC
  ATCGATGATATGAGATTTATTATGGATAAAATTCCAGCAGTACAACGTCAAACAATGTTG
  TTCTCAGCTACAATGCCTAAAGCAATCCAAGCTTTAGTACAACAATTTATGAAATCACCA
  AAAATCATTAAGACAATGAATAATGAAATGTCTGATCCACAAATCGAAGAATTCTATACA
  ATTGTTAAAGAATTAGAGAAATTTGATACATTTACAAATTTCCTAGATGTTCATCAACCT
  GAATTAGCAATCGTATTCGGACGTACAAAACGTCGTGTTGATGAATTAACAAGTGCTTTG
  ATTTCTAAAGGATATAAAGCTGAAGGTTTACATGGTGATATTACACAAGCGAAACGTTTA
  GAAGTATTAAAGAAATTTAAAAATGACCAAATTAATATTTTAGTCGCTACTGATGTAGCA
  GCAAGAGGACTAGATATTTCTGGTGTGAGTCATGTTTATAACTTTGATATACCTCAAGAT
  ACTGAAAGCTATACTCACCGTATTGGTCGTACGGGTCGTGCTGGTAAAGAAGGTATCGCT
  GTAACGTTTGTTAATCCAATCGAAATGGATTATATCAGACAAATTGAAGATGCAAATGGT
  AGAAAAATGAGTGCACTTCGTCCACCTCATCGTAAAGAAGTACTTCAAGCACGTGAAGAT
  GACATCAAAGAAAAAGTTGAAAACTGGATGTCTAAAGAGTCAGAATCACGCTTGAAACGT
  ATTTCTACAGAGTTGTTAAATGAATATAACGATGTTGATTTAGTTGCTGCACTTTTACAA
  GAGTTAGTAGAAGCGAACGATGAAGTTGAAGTTCAATTAACTTTTGAAAAACCATTATCT
  CGCAAAGGCCGTAATGGTAAACCAAGCGGTTCTCGTAACAGAAATAGTAAGCGTGGTAAT
  CCTAAATTTGACAGTAAGAGTAAACGTTCAAAAGGATACTCAAGTAAAAAGAAAAGTACA
  AAAAAATTCGACCGTAAAGAGAAGAGCAGCGGTGGAAGCAGACCTATGAAAGGTCGCACA
  TTTGCTGACCATCAAAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1521
  

⊟Protein

⊟General

• locus tag: JSNZ_002042
• symbol: JSNZ_002042
• description: DEAD/DEAH box helicase
• length: 506
• theoretical pI: 10.144
• theoretical MW: 56942
• GRAVY: -0.578854

⊟Function

• reaction:
  EC 3.6.4.-?  ExPASy
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 87)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 75.2)
  and 11 more
  DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 54.8)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 46)
  helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 41.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 36.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair reverse gyrase (TIGR01054; EC 3.6.4.12,5.99.1.3; HMM-score: 31.3)
  CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 26.6)
  DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 21.1)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 19.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 18.4)
  CRISPR-associated helicase Cas3, subtype CYANO (TIGR03158; HMM-score: 12.9)
  Unknown function Enzymes of unknown specificity ATP-dependent helicase HrpB (TIGR01970; EC 3.6.4.-; HMM-score: 10.8)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 164.3)
  and 7 more
  Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 118.3)
  ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 28.2)
  CMS1; U3-containing 90S pre-ribosomal complex subunit (PF14617; HMM-score: 16.8)
  Cas3-like_C_2; CRISPR-associated nuclease/helicase Cas3, C-terminal (PF22590; HMM-score: 14.4)
  AAA_19; AAA domain (PF13245; HMM-score: 13.8)
  AAA_30; AAA domain (PF13604; HMM-score: 13.5)
  Flavi_DEAD; Flavivirus DEAD domain (PF07652; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9925
  • Cytoplasmic Membrane Score: 0.0037
  • Cell wall & surface Score: 0.0011
  • Extracellular Score: 0.0027
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.021021
  • TAT(Tat/SPI): 0.001746
  • LIPO(Sec/SPII): 0.001025
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILGQAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVTVFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGFIDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEFYTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQAKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRAGKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSKESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSGSRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications